A5048290667- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Lysosomal Storage Disorders Research
- Cellular transport and secretion
- Viral gastroenteritis research and epidemiology
- Salmonella and Campylobacter epidemiology
- Immune Response and Inflammation
- Listeria monocytogenes in Food Safety
- Antimicrobial Peptides and Activities
- Autoimmune and Inflammatory Disorders Research
- Veterinary medicine and infectious diseases
- Endoplasmic Reticulum Stress and Disease
University of Lisbon
2014-2021
Cardiff University
2019-2021
Instituto Politécnico de Lisboa
2016-2017
Instituto Nacional de Investigação Agrária e Veterinária
2012
Sport Lisboa e Benfica
2012
Nystatin-induced membrane permeabilization is related to its effects on properties and organization.
Glucosylceramide (GlcCer) plays an active role in the regulation of various cellular events. Moreover, GlcCer is also a key modulator membrane biophysical properties, which might be linked to mechanism its biological action. In order understand implications on membranes living cells, we first studied effect artificial containing 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), sphingomyelin (SM) and cholesterol (Chol). Using array methods, demonstrate that at lower GlcCer/Chol...
Abstract Synthetic systems are widely used to unveil the molecular mechanisms of complex cellular events. Artificial membranes key examples models employed address lipid-lipid and lipid-protein interactions. In this work, we developed a new synthetic system that more closely resembles lysosome – lysosome-mimicking vesicles (LMVs) displaying stable acid-to-neutral pH gradient across membrane. To evaluate advantages system, assessed distinct effects sphingosine (Sph) accumulation in membrane...
Sphingosine (Sph) is a simple lipid involved in the regulation of several biological processes. When accumulated late endosomal/lysosomal compartments, Sph causes changes ion signaling and membrane trafficking, leading to development Niemann-Pick disease type C. Little known about interaction with other lipids membranes; however, understanding effect physical state membranes might provide insights into its mode action. Using complementary established fluorescence approaches, we show that...