A5030458055- Lipid Membrane Structure and Behavior
- Sphingolipid Metabolism and Signaling
- Erythrocyte Function and Pathophysiology
- Surfactants and Colloidal Systems
- Chemical Synthesis and Analysis
- Immunotherapy and Immune Responses
- Lysosomal Storage Disorders Research
- Antimicrobial Peptides and Activities
- Cellular transport and secretion
- RNA Interference and Gene Delivery
- Dendrimers and Hyperbranched Polymers
- Polydiacetylene-based materials and applications
- Lipid metabolism and biosynthesis
- Molecular Sensors and Ion Detection
- Nanoparticle-Based Drug Delivery
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Luminescence and Fluorescent Materials
- Photosynthetic Processes and Mechanisms
- Advanced Software Engineering Methodologies
- Plant biochemistry and biosynthesis
- Pharmaceutical Economics and Policy
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Nanoparticles: synthesis and applications
University of Lisbon
2015-2024
Institute for Biotechnology and Bioengineering
2019
Lusíada University of Lisbon
2018
Instituto Politécnico de Lisboa
2016-2017
Weizmann Institute of Science
2008-2012
Instituto Superior Técnico
2003-2012
Faculdade Meridional
2011
Universidade de Pernambuco
2008-2010
Instituto de Engenharia de Sistemas e Computadores Microsistemas e Nanotecnologias
2009
Instituto de Engenharia de Sistemas e Computadores Investigação e Desenvolvimento
2008
Ceramide is an important lipid signaling molecule that plays critical roles in regulating cell behavior. synthesis surprisingly complex and orchestrated by six mammalian ceramide synthases, each of which produces ceramides with restricted acyl chain lengths. We have generated a CerS2 null mouse characterized the changes long base sphingolipid composition livers from these mice. downstream sphingolipids were devoid very (C22-C24) chains, consistent substrate specificity toward acyl-CoAs....
Abstract Breast cancer is the primary cause of cancer‐related death in women worldwide. subtypes are characterized by different gene expression patterns, which drive their prognostic factors and therapeutic options. Among them, triple‐negative breast (TNBC) one deadliest due to its aggressiveness, high rate early recurrence distant metastases, limited Despite recent approval monoclonal antibodies targeting programmed cell protein 1 (PD‐1) or ligand (PD‐L1) for treatment TNBC patients with a...
To understand the formation and properties of ceramide-enriched domains in cell membranes, biophysical binary system palmitoyloleoylphosphatidylcholine (POPC)/palmitoylceramide were thoroughly characterized. Diverse fluorescent probes parameters necessary to unravel complexity this apparently simple system. For first time, a complete phase diagram is reported, characterizing lamellar phases these mixtures, providing quantitative framework integrating biological studies. The suggests that...
Abstract The modular assembly of boronic acids with Schiff‐base ligands enabled the construction innovative fluorescent dyes [boronic acid salicylidenehydrazone (BASHY)] suitable structural and photophysical properties for live cell bioimaging applications. This reaction straightforward synthesis (yields up to 99 %) structurally diverse photostable that exhibit a polarity‐sensitive green‐to‐yellow emission high quantum yields 0.6 in nonpolar environments. These displayed brightness (up 54...
In previous studies we showed that the replication of Cryptococcus neoformans in lung environment is controlled by glucosylceramide (GlcCer) synthase gene (GCS1), which synthesizes membrane sphingolipid GlcCer from C9-methyl ceramide. Here, studied effect mutation C9 methyltransferase (SMT1), adds a methyl group to position 9 sphingosine backbone The C. Δsmt1 mutant does not make ceramide and, thus, any methylated GlcCer. However, it accumulates demethylated and loses more than 80% its...
Little is known about the effects of altering sphingolipid (SL) acyl chain structure and composition on biophysical properties biological membranes. We explored consequences depleting very long (VLC) SLs in membranes prepared from lipid fractions isolated a ceramide synthase 2 (CerS2)-null mouse, which unable to synthesize C22–C24 ceramides. demonstrate that ablation CerS2 has different liver brain, causing significant alteration fluidity membrane affecting type and/or extent phases present...
Ceramide (Cer) is involved in the regulation of several cellular processes by mechanisms that depend on Cer-induced changes membrane biophysical properties. Accumulating evidence shows Cers with different N-acyl chain composition differentially impact cell physiology, which may part be due to specific alterations We now address how sphingolipid (SL) affects properties cultured human embryonic kidney cells overexpressing Cer synthases (CerSs). Our results show an increase order membranes...
Background and Aims: Receptor‐interacting protein kinase 3 (RIPK3) mediates NAFLD progression, but its metabolic function is unclear. Here, we aimed to investigate the role of RIPK3 in modulating mitochondria function, coupled with lipid droplet (LD) architecture NAFLD. Approach Results: Functional studies evaluating LD biology were performed wild‐type (WT) Ripk3 −/− mice fed a choline‐deficient, amino acid‐defined (CDAA) diet for 32 66 weeks CRISPR‐Cas9 ‐null fat‐loaded immortalized...
Abstract Immune checkpoint blockade reaches remarkable clinical responses. However, even in the most favorable cases, half of these patients do not benefit from therapies long term. It is hypothesized that activation host immunity by co‐delivering peptide antigens, adjuvants, and regulators transforming growth factor (TGF)‐β expression using a polyoxazoline (POx)‐poly(lactic‐ co ‐glycolic) acid (PLGA) nanovaccine, while modulating tumor‐associated macrophages (TAM) function within tumor...
Nystatin-induced membrane permeabilization is related to its effects on properties and organization.
This paper presents the results of an exploratory study on fault-proneness aspect-oriented programs. We analysed faults collected from three evolving systems, all different application domains. The analysis develops two angles. Firstly, we measured impact obliviousness property evaluated systems. show that 40% reported were due to lack awareness among base code and aspects. second regarded main programming (AOP) mechanisms, namely pointcuts, advices intertype declarations. indicate these...