A5100637938- Brain Metastases and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Parasites and Host Interactions
- Glioma Diagnosis and Treatment
- Neurogenesis and neuroplasticity mechanisms
- Lung Cancer Treatments and Mutations
- Immune cells in cancer
- Cancer Genomics and Diagnostics
- Parasite Biology and Host Interactions
- Lung Cancer Research Studies
- Trace Elements in Health
- Anesthesia and Neurotoxicity Research
- Bone Metabolism and Diseases
- Research on Leishmaniasis Studies
- TGF-β signaling in diseases
- Immune Response and Inflammation
- Helminth infection and control
- Signaling Pathways in Disease
- Neuroscience and Neuropharmacology Research
- Renal and related cancers
- Vitamin K Research Studies
- Stroke Rehabilitation and Recovery
- Endoplasmic Reticulum Stress and Disease
- Nuclear Structure and Function
- Pluripotent Stem Cells Research
Karolinska Institutet
2015-2025
Minia University
2024-2025
Sudan Medical Specialization Board
2024
University of Sadat City
2024
Memorial Sloan Kettering Cancer Center
2021-2024
Cairo University
1994-2024
Aswan University
2024
Nationwide Children's Hospital
2023
Najran University
2022
Hamad Medical Corporation
2022
Inflammatory response induced by microglia plays a critical role in the demise of neuronal populations neuroinflammatory diseases. Although toll-like receptor 4 (TLR4) microglia's inflammatory is fully acknowledged, little known about endogenous ligands that trigger TLR4 activation. Here, we report galectin-3 (Gal3) released acts as an paracrine ligand. Gal3-TLR4 interaction was further confirmed murine model (intranigral lipopolysaccharide [LPS] injection) and human stroke subjects....
Microglial phagocytosis of apoptotic debris prevents buildup damage neighbor neurons and inflammatory responses. Whereas microglia are very competent phagocytes under physiological conditions, we report their dysfunction in mouse preclinical monkey models stroke (macaques marmosets) by transient occlusion the medial cerebral artery (tMCAo). By analyzing recently published bulk single cell RNA sequencing databases, show that was not explained transcriptional changes. In contrast, demonstrate...
Molecular diversity of microglia, the resident immune cells in CNS, is reported. Whether microglial subsets characterized by expression specific proteins constitute subtypes with distinct functions has not been fully elucidated. Here we describe a subtype expressing enzyme arginase-1 (ARG1; that is, ARG1+ microglia) found predominantly basal forebrain and ventral striatum during early postnatal mouse development. microglia are enriched phagocytic inclusions exhibit molecular signature,...
Members of transforming growth factor-beta (TGF-β) superfamily play pivotal roles in development multicellular organisms. We report the functional characterization Schistosoma mansoni type II receptor (SmTβRII). Mining S. expressed sequence tag (EST) database identified an EST clone that shows homology to kinase domain receptors from different species. The amplified was used as a probe isolate cDNA spanning entire coding region serine/threonine receptor. interaction SmTβRII with SmTβRI...
The mechanisms of neuronal injury after hypoxia–ischemia (HI) are different in the immature and adult brain, but microglia activation has not been compared. purpose this study was to phenotype resident blood-derived macrophages hippocampus HI neonatal (postnatal day 9, P9) or (3 months age, 3mo) mice. Unilateral brain induced Cx3cr1GFP/+Ccr2RFP/+ male mice on P9 (n = 34) at 3mo 53) using Vannucci model. Resident (Cx3cr1-GFP+) proliferated were activated earlier (1–3 days) than that...
Cortical ischemia induces neural progenitor cell migration toward the injury site; however, whether these cells are capable of maintaining migratory response for a longer period after remains uncertain.We analyzed up to 1 year induction photothrombotic stroke mouse neocortex. Migrating progenitors identified as doublecortin positive (DCX+) were assessed using immunohistochemistry and immunofluorescence. The thymidine analogues chlorodeoxyuridine iododeoxyuridine used birth-date cells.In...
Cranial irradiation (IR), an effective tool to treat malignant brain tumors, triggers a chronic pro-inflammatory microglial response, at least in the adult brain. Using single-cell and bulk RNA sequencing, combined with histology, we show that response juvenile mouse hippocampus is rapid but returns toward normal within 1 week. The characterized by series of temporally distinct homeostasis-, sensome-, inflammation-related molecular signatures. We find single cell simultaneously upregulates...
Abstract Metastasis to the cerebrospinal fluid (CSF)-filled leptomeninges, or leptomeningeal metastasis (LM), represents a fatal complication of cancer. Proteomic and transcriptomic analyses human CSF reveal substantial inflammatory infiltrate in LM. We find solute immune composition setting LM changes dramatically, with notable enrichment IFN-γ signaling. To investigate mechanistic relationships between cell signaling cancer cells within we developed syngeneic lung, breast, melanoma mouse...
Schistosoma mansoni, a multicelluar eukaryotic blood fluke, is major cause of morbidity worldwide in humans. The study female parasite growth, development, and gene regulation important because the eggs produced are responsible for pathogenesis observed schistosomiasis. p14, an eggshell precursor expressed only sexually mature females response to male stimulus, model female-specific regulation. upstream region p14 shares sequences present insect genes known be regulated sex-, temporal-,...
Smad proteins are essential intracellular signal transducers of the transforming growth factor-β (TGF-β) superfamily. The TGF-β superfamily signals through phosphorylation and activation R-Smad proteins, receptor-regulated Smads, by heteromeric complexes ligand-specific type I II serine/threonine kinase receptors. R-Smads receive a from activated receptor complex transmit it to nucleus. A cDNA was isolated that encodes 649-amino acid protein found be homologous members subfamily with highest...
Cranial radiotherapy causes progressive neurocognitive impairments in cancer survivors. Neuroinflammation is believed to be a key contributor, but its dynamics and consequences for brain function remain poorly understood. Here, we performed comprehensive longitudinal profiling, from 6 hours 1 year after irradiation (IR) of the mouse hippocampus, using transcriptomic, protein histological analyses. We identified delayed microglial response coupling interferon signaling mitotic progression,...
Although many interventions have been proposed to reduce morbidity and mortality following a transient ischemic attack or stroke, the optimal procedure is controversial. Since dual antiplatelet therapy with clopidogrel aspirin alone are widely used for people who experienced an we sought identify benefits risks of each intervention provide healthcare provider objective evidence use in construct comprehensive treatment plans. We conducted systematic review meta-analysis assess vs ....
Schistosome parasites are muticellular eucaryotic organisms with a complex life cycle that involves mammalian and snail hosts. Unlike other trematode parasites, schistosomes (along the Didymozoidae) have evolved separate sexes or dioecy. Sex is determined by chromosomal mechanism. The dioecious state created an opportunity for to play role in schistosome evolution has resulted interesting interplay between sexes. classical observation, made more than 50 years ago, female do not develop...
Fluorescent reporter labeling and promoter-driven Cre-recombinant technologies have facilitated cellular investigations of physiological pathological processes, including the widespread use Cx3cr1CreER-Eyfp/wt mouse strain for studies microglia.Immunohistochemistry, Flow Cytometry, RNA sequencing whole-genome were used to identify subpopulation microglia in brains. Genetically mediated depletion using Cx3cr1CreER-Eyfp/wtRosa26DTA/wt mice CSF1 receptor inhibitor PLX3397 deplete microglia....
Members of the transforming growth factor (TGF)-β superfamily play pivotal roles in cell migration, differentiation, adhesion, pattern formation, and apoptosis. The family Smad proteins acts as intracellular signal transducers TGF-β related peptides. Smad4, a common mediator (co-Smad), performs central role transmitting signals from TGF-β, BMP, activins. Schistosoma mansoni receptor-regulated Smad1 SmSmad2 were previously identified shown to act signaling. Herein, we report identification...
ADP-ribosyl cyclases are structurally conserved enzymes that best known for catalyzing the production of calcium-mobilizing metabolite, cyclic adenosine diphosphate ribose (cADPR), from nicotinamide adenine dinucleotide (NAD+). However, these also produce (ADPR) and nicotinic acid phosphate (NAADP+), both which have been shown to modulate calcium mobilization in vitro. We now characterized a new member cyclase family Schistosoma mansoni, Platyhelminthes phylum. show novel NAD(P)+...