A5040836469- Neuroinflammation and Neurodegeneration Mechanisms
- Neurobiology and Insect Physiology Research
- Autophagy in Disease and Therapy
- Immune cells in cancer
- Epigenetics and DNA Methylation
- Nerve injury and regeneration
- Intracerebral and Subarachnoid Hemorrhage Research
- Invertebrate Immune Response Mechanisms
- Genetics, Aging, and Longevity in Model Organisms
- Corneal Surgery and Treatments
- Neurogenesis and neuroplasticity mechanisms
- RNA Interference and Gene Delivery
- Plant Molecular Biology Research
- Microbial Metabolic Engineering and Bioproduction
- Hippo pathway signaling and YAP/TAZ
- Zebrafish Biomedical Research Applications
- Periodontal Regeneration and Treatments
- Spinal Hematomas and Complications
- Neurological Disease Mechanisms and Treatments
- Neuroscience and Neuropharmacology Research
- dental development and anomalies
- Biofuel production and bioconversion
- Cellular transport and secretion
- Histone Deacetylase Inhibitors Research
- Sirtuins and Resveratrol in Medicine
University of Helsinki
2012-2025
Karolinska Institutet
2017-2023
Molecular diversity of microglia, the resident immune cells in CNS, is reported. Whether microglial subsets characterized by expression specific proteins constitute subtypes with distinct functions has not been fully elucidated. Here we describe a subtype expressing enzyme arginase-1 (ARG1; that is, ARG1+ microglia) found predominantly basal forebrain and ventral striatum during early postnatal mouse development. microglia are enriched phagocytic inclusions exhibit molecular signature,...
Epigenomic mechanisms regulate distinct aspects of the inflammatory response in immune cells. Despite central role for microglia neuroinflammation and neurodegeneration, little is known about their epigenomic regulation response. Here, we show that Ten-eleven translocation 2 (TET2) methylcytosine dioxygenase expression increased upon stimulation with various inflammogens through a NF-κB-dependent pathway. We found TET2 regulates early gene transcriptional changes, leading to metabolic...
Abstract During intracerebral hemorrhage (ICH), hematoma formation at the site of blood vessel damage results in local mechanical injury. Subsequently, erythrocytes lyse to release hemoglobin and heme, which act as neurotoxins induce inflammation secondary brain injury, resulting severe neurological deficits. Accelerating resorption mitigating hematoma-induced edema by modulating immune cells has potential a novel therapeutic strategy for functional recovery after ICH. Here, we show that...
The peri-infarct region after ischemic stroke is the anatomical location for many of endogenous recovery processes; however, -the molecular events in remain poorly characterized. In this study, we examine profile on post-stroke day four, a time when reparative processes are ongoing. We used multiomics approach, involving RNA sequencing, and mass spectrometry-based proteomics metabolomics to characterize changes region. also took advantage our previously developed method express transgenes...
Multi-domain proteins, comprising both folded and intrinsically disordered regions, play pivotal roles in various biological processes, yet characterizing their conformational ensembles dynamics presents significant challenges. Here we address this challenge by introducing the Quality Evaluation Based Simulation Selection (QEBSS) protocol that enables detailed interpretation of multi-domain proteins using a combination MD simulations protein backbone 15N T1 T2 spin relaxation times hetNOE...
Glia are abundant cells in the brain of animals ranging from flies to humans. They perform conserved functions not only neural development and wiring, but also homeostasis. Here we show that by manipulating gene expression glia, a previously unidentified cell type appears Drosophila during metamorphosis. More specifically, this three contexts: (1) after induction either immunity, or (2) autophagy, (3) silencing neurotrophic factor DmMANF glial cells. We call these MANF immunoreactive Cells...
High-grade gliomas are malignant aggressive primary brain tumors with limited therapeutic options, and dismal prognosis for patients. Microglia, the resident immune cells of brain, recruited reprogrammed into tumor-supporting by glioma cells, which in turn positively influence tumor expansion infiltration surrounding tissues. Here, we report that glioma-induced microglia conversion is coupled to an increase histone H4 lysine 16 (H4K16) acetylation level microglia, through increased nuclear...
Understanding the control of stem cell (SC) differentiation is important to comprehend developmental processes as well develop clinical applications. Lin28 a conserved molecule that involved in SC maintenance and by regulating let-7 miRNA maturation. However, little known about vivo function Lin28. Here, we report critical roles for lin-28 during oogenesis. We found maturation was increased null mutant fly ovaries. showed female flies displayed reduced fecundity, due defects egg chamber...
Abstract Macroautophagy/autophagy is an evolutionarily conserved and tightly regulated catabolic process involved in the maintenance of cellular homeostasis whose dysregulation implicated several pathological processes. Autophagy begins with formation phagophores that engulf cytoplasmic cargo mature into double-membrane autophagosomes; latter fuse lysosomes/vacuoles for degradation recycling. Here, we report yeast Set2, a histone lysine methyltransferase, its mammalian homolog, SETD2, both...
In Drosophila melanogaster, mesencephalic astrocyte-derived neurotrophic factor (DmMANF) is an evolutionarily conserved orthologue of mammalian MANF and cerebral dopamine (CDNF), which have been shown to promote the survival dopaminergic neurons in brain. We observed especially high levels DmMANF visual system Drosophila, particularly first optic neuropil (lamina). lamina, was found glial cells (surface epithelial glia), photoreceptors interneurons. Interestingly, silencing all or...
Background Glial cell line-derived neurotrophic factor (GDNF) family ligands are secreted growth factors distantly related to the TGF-β superfamily. In mammals, they bind GDNF receptor α (Gfrα) and signal through Ret tyrosine kinase. order gain insight into evolution of Ret-Gfr-Gdnf signaling system, we have cloned characterized first invertebrate Gfr-like cDNA (DmGfrl) from Drosophila melanogaster generated a DmGfrl mutant allele. Results We found that encodes large GPI-anchored membrane...
The cornea, transparent and outermost structure of camera-type eyes, is prone to environmental challenges, but has remarkable wound healing capabilities which enables preserve vision. manner in cell plasticity impacts remains be determined. In this study, we report rapid closure after zebrafish corneal epithelium abrasion. Furthermore, by investigating the cellular molecular events taking place during epithelial closure, show induction a bilateral response unilateral wound. Our...
Summary Epigenetic mechanisms regulate distinct aspects of the inflammatory response in various immune cell types. Despite central role for microglia, resident macrophages brain, neuroinflammation and neurodegeneration little is known about their epigenetic regulation response. Here, we show that Ten-eleven translocation 2 (TET2) methylcytosine dioxygenase expression increased microglia upon stimulation with inflammogens through a NF-κB-dependent pathway. We found TET2 regulates early gene...
Abstract Most of terrestrial and aquatic vertebrates are equipped with camera-type eyes, offering a focused clear sight. This apparatus is rendered inefficient if its most superficial transparent element, the cornea, opaque. structure, prone to environmental aggressions, bears excellent wound healing capabilities preserve vision. Up date, corneal studies made on mammals. Here, for first time, zebrafish used as model study closure epithelium after abrasion. Our demonstrates swift insult....
Mesencephalic Astrocyte-derived Neurotrophic Factor (MANF) is one of a few neurotrophic factors described in Drosophila melanogaster (DmMANF) but its function still poorly characterized. In the present study we found that DmMANF expressed different clusters clock neurons. particular, PDF-positive large (l-LNv) and small (s-LNv) ventral lateral neurons, CRYPTOCHROME-positive dorsal neurons (LNd), group 1 posterior (DN1p) tim -positive cells fly’s visual system. Importantly, expression not...
Abstract Diversity within microglia, the resident brain immune cells, is reported. Whether microglial subsets constitute different subtypes with intrinsic properties and unique functions has not been fully elucidated. Here, we describe a subtype characterized by expression of enzyme Arginase-1, i.e. Arg1 + which found predominantly in cholinergic neuron-rich forebrain region during early postnatal development. microglia are frequently observed close apposition to neurons exhibit distinctive...
Recent advances in bioengineering and biomaterials, along with knowledge deriving from the fields of developmental biology stem cell research, have rendered feasible functional replacement full organs. Here, we describe methodology for a tooth, starting embryonic epithelial mesenchymal single suspensions. In addition, subsequent steps processing this minute structure use applications such as histological examination, immunofluorescence situ hybridisation. This can be used any that needs to...
Abstract During intracerebral hemorrhage (ICH), hematoma formation at the site of blood vessel damage results in local mechanical injury. Subsequently, erythrocytes lyse to release hemoglobin and heme, which act as neurotoxins induce inflammation secondary brain injury, resulting severe neurological deficits. Accelerating resorption mitigating hematoma-induced edema by modulating immune cells has potential a novel therapeutic strategy for functional recovery after ICH. Here, we show that...