A5062905106- Antifungal resistance and susceptibility
- Fungal Biology and Applications
- Peptidase Inhibition and Analysis
- Mycotoxins in Agriculture and Food
- Fungal and yeast genetics research
- Microbial Natural Products and Biosynthesis
- Fungal Infections and Studies
- Veterinary medicine and infectious diseases
- Parasitic Infections and Diagnostics
- Infectious Diseases and Mycology
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Coccidia and coccidiosis research
- Entomopathogenic Microorganisms in Pest Control
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Synthesis and Biological Evaluation
- Indoor Air Quality and Microbial Exposure
- Advanced biosensing and bioanalysis techniques
- Trace Elements in Health
- Proteoglycans and glycosaminoglycans research
- SARS-CoV-2 and COVID-19 Research
- Ocular Infections and Treatments
- Radiopharmaceutical Chemistry and Applications
- Microbial infections and disease research
- Plant Pathogens and Fungal Diseases
University of Tennessee Health Science Center
2023-2024
University of the Basque Country
2014-2022
Rostov Research Institute of Microbiology and Parasitology
2020
Abstract Triazoles, the most widely used class of antifungal drugs, inhibit biosynthesis ergosterol, a crucial component fungal plasma membrane. Inhibition separate ergosterol biosynthetic step, catalyzed by sterol C-24 methyltransferase Erg6, reduces virulence pathogenic yeasts, but its effects on filamentous pathogens like Aspergillus fumigatus remain unexplored. Here, we show that lipid droplet-associated enzyme Erg6 is essential for viability A. and other species, including lentulus ,...
Abstract Triazole antifungals function as ergosterol biosynthesis inhibitors and are frontline therapy for invasive fungal infections, such aspergillosis. The primary mechanism of action triazoles is through the specific inhibition a cytochrome P450 14-α-sterol demethylase enzyme, Cyp51A/B, resulting in depletion cellular ergosterol. Here, we uncover clinically relevant secondary within pathway. We provide evidence that triazole-mediated Cyp51A/B activity generates sterol intermediate...
Virulence mechanisms of the pathogenic fungus Aspergillus fumigatus are multifactorial and depend on immune state host, but little is known about fungal mechanism that develops during process lung invasion. In this study, microarray technology was combined with a histopathology evaluation infected lungs so invasion strategy followed by could be described. To achieve this, an intranasal mice infection performed to extract daily samples from over four days post-infection. The pathological...
ABSTRACT Aspergillus fumigatus is a major invasive mold pathogen and the most frequent etiologic agent of aspergillosis. The currently available treatments for aspergillosis are limited in both number efficacy. Our recent work has uncovered that β-glucan synthase inhibitors, echinocandins, fungicidal against strains A. with defects septation initiation network (SIN) kinase activity. These drugs known to be fungistatic normal septation. Surprisingly, SIN mutant also failed invade lung tissue...
Aspergillus fumigatus is considered to be the most prevalent airborne pathogenic fungus and can cause invasive diseases in immunocompromised patients. It known that its virulence multifactorial, although mechanisms of pathogenicity remain unclear. With aim improving our understanding these mechanisms, we designed a new expression microarray covering entire genome A. fumigatus. In this first study, analysed transcriptomes at steps germination after being grown 24 37 °C. The data revealed 1249...
Fungal pathogens must exhibit strong nutritional plasticity, effectively sensing and utilizing diverse nutrients to support virulence. How the signals generated by are efficiently translated morphogenetic machinery for optimal growth of virulence remains incompletely understood. Here, we show that conserved morphogenesis-related kinase, CotA, imparts isoform-specific control over Aspergillus fumigatus invasive in host-mimicking environments during infection. CotA-mediated is responsive...
ABSTRACT The SARS-CoV-2 pandemic has evolved far more aggressively in countries lacking a robust testing strategy to identify infected individuals. Given the global demand for fast and reliable diagnosis determine carrier individuals, stock-out scenario number of essential reagents/kits used along diagnostic process been foreseen by many organizations. Having identified RNA extraction step as one key bottlenecks, we tested several alternatives that avoid use commercial kits this step....
Lomentospora (Scedosporium) prolificans is an opportunistic pathogen capable of causing invasive infections in immunocompromised patients. The fungus able to disseminate via the bloodstream finally arriving at central nervous system producing neurological symptoms and, many cases, patient death. In this context, microglial cells, which are resident immune cells system, may play important role these infections. However, aspect anti-L. immunity has been poorly researched date. Thus,...
The activity of fumagillin, a mycotoxin produced by Aspergillus fumigatus, has not been studied in depth. In this study, we used commercial fumagillin on cultures two cell types (A549 pneumocytes and RAW 264.7 macrophages). This toxin joins its target, MetAP2 protein, inside cells and, as result, significantly reduces the electron chain activity, migration, proliferation ability A549 cells, or affects viability macrophages. However, stimulates germination double branch hypha production...
Ergosterol is a critical component of fungal plasma membranes. Although many currently available antifungal compounds target the ergosterol biosynthesis pathway for effect, current knowledge regarding synthesis remains incomplete filamentous pathogens like
In this study, two distinct in vitro infection models of Aspergillus fumigatus , using murine macrophages (RAW264.7) and human lung epithelial cells (A549), were employed to identify the genes important for fungal adaptation during infection. Transcriptomic analyses co-incubated A. uncovered 140 up-regulated common between both that, when compared with a previously published vivo transcriptomic allowed identification 13 consistently all three conditions. Among them, maiA gene, responsible...
Quantitative reverse transcription PCR (RT-qPCR) is the leading tool to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Given that it will almost certainly continue coexist with other viruses in coming years, our study aimed design a multiplex system not affected by supplier outages and reduced cost compared existing commercially available kits.In this study, combinations of four primers/probe sets were used construct flexible RT-qPCR assay which capable discriminating...
Fumagillin is a biomolecule produced by Aspergillus fumigatus that gaining relevance due to its connection with invasive aspergillosis. The determination of this molecule might help understand the propagation disease and study use as potential biomarker. In spite interest fumagillin in microbiological research, no quantitative method has been developed so far for cell culture media. work, first validated analysis RPMI-1640 presented. sample treatment consists mixed-mode anion exchange Solid...
Treatment of invasive fungal infections with Caspofungin is used as the first-line antifungal agents. The minimum inhibitory concentration value a test which indicates degree sensitivity strain regarding drug. However, no for caspofungin available because very variable obtained. In this work, we study link adsorption phenomenon CSF previously described in literature and lack value. A systematic impact different parameters on reported. effect nature container material, aqueous solution pH...
Aspergillus fumigatus onddoak sortutako aspergilosi inbaditzailea mehatxua da immunoeskasia duten gaixoentzat. Azkeneko ikerketa batzuen arabera, fumagilinak, mikotoxinak, gaixotasunaren hedapenarekin zerikusia duela ikusi da. Hori dela eta, konposatu honen determinazioa lagungarria izan daiteke bai mekanismoak hobeto ulertzeko eta baita inbaditzailearen biomarkatzaile gisa erabili ahal izateko ere. Ikerketa mikrobiologikoetan fumagilinaren analisiak garrantzia arren, oraindik ez haren...
Abstract The genus Aspergillus includes industrially, medically and agriculturally important species. All of them, as do fungi in general, disperse to new niches principally by means asexual spores. Regarding the genetic/molecular control development, nidulans is main reference. In this species, two pathways production conidiophores, structures bearing spores (conidia). Upstream Developmental Activation (UDA) pathway transduces environmental signals, determining whether Central Pathway (CDP)...
Abstract In this study, two distinct in vitro infection models of Aspergillus fumigatus , using murine macrophages (RAW264.7) and human lung epithelial cells (A549), were employed to identify the genes important for fungal adaptation during infection. Transcriptomic analyses co-incubated uncovered 140 up-regulated common between both that, when compared with a previously published vivo transcriptomic allowed identification 13 consistently all three conditions. Among them, maiA gene,...
ABSTRACT Asexual spores are the main vehicle used by fungi to disperse new niches. The Eurotiomycete Aspergillus nidulans is reference for study of genetic/molecular control asexual development. In this species, Flb proteins expression master gene brlA, and thus, loss-of-function mutations in flb (upstream developmental activation [UDA]) genes block brlA transcription and, consequently, production conidiophores, structures bearing known as conidia. However, aconidial phenotype specific...
Ikerketa askok mikroorganismoen eta minbizien arteko erlazioak aztertu dituzte, erakutsi dute mikroorganismo batzuek minbiziaren agerpena saihesten dutela beste batzuek, aldiz, minbizia eragin dezaketela. Hain zuzen ere, gero artikulu zientifiko gehiago argitaratzen ari dira mikroorganismoak sortzearekin, ezarpenarekin sakabanaketarekin erlazionatuz. Izan mikroorganismoek minbizi guztien % 17,8 eragiten estimatu da. Minbizia sortzeko birusen gaitasuna da gehien ikertu dena eta, ondorioz, sor...