A5066621384- Cellular Mechanics and Interactions
- Connexins and lens biology
- 3D Printing in Biomedical Research
- Heat shock proteins research
- Cell Adhesion Molecules Research
- Skin and Cellular Biology Research
- Yersinia bacterium, plague, ectoparasites research
- Cell Image Analysis Techniques
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microtubule and mitosis dynamics
- Caveolin-1 and cellular processes
- Monoclonal and Polyclonal Antibodies Research
- Nanofabrication and Lithography Techniques
- Glioma Diagnosis and Treatment
- Wnt/β-catenin signaling in development and cancer
- Additive Manufacturing and 3D Printing Technologies
- Neurogenesis and neuroplasticity mechanisms
- Peptidase Inhibition and Analysis
- Single-cell and spatial transcriptomics
- Cystic Fibrosis Research Advances
- Galectins and Cancer Biology
- Biomedical Ethics and Regulation
- S100 Proteins and Annexins
- Click Chemistry and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
Institut Interdisciplinaire de Neuroscience
2015-2024
Centre National de la Recherche Scientifique
2008-2024
Alvéole (France)
2018-2024
Université de Bordeaux
2015-2024
Okanagan University College
2019
University of British Columbia
2019
National University of Singapore
2017
Université Paris Cité
2015-2017
Institut Jacques Monod
2015-2017
Institut du Fer à Moulin
2014
Light-induced molecular adsorption of proteins (LIMAP) allows for quantitative sub-micrometer-resolution printing multiple biomolecules. Surface-bound gradients are patterned within minutes over an entire glass cover-slip. LIMAP is used to perform selective immuno-assays, dynamically control the adhesion individual cells, and achieve hierarchical co-cultures instrumental tissue engineering.
The shaping of a multicellular body and repair adult tissues require fine--tuning cell adhesion, mechanics, intercellular transmission mechanical load. Adherens junctions (AJs) are the major by which cells sense exert force on each other. However, how AJs adapt to stress this adaptation contributes cell-cell cohesion eventually tissue-scale dynamics mechanics remains largely unknown. Here, analyzing tension-dependent recruitment vinculin, α-catenin, F-actin as function stiffness, well...
Oligomerization of cadherins could provide the stability to ensure tissue cohesion. Cadherins mediate cell–cell adhesion by forming trans-interactions. They form cis-interactions whose role be essential stabilize intercellular junctions shifting cadherin clusters from a fluid an ordered phase. However, no evidence has been provided so far for oligomerization in cellulo and its impact on contact stability. Visualizing single within cell membrane at nanometric resolution, we show that...
Glioblastoma cells are characterized by high proliferation and invasive capacities. Tumor development has been associated with a decrease of gap-junctional intercellular communication, but the concrete involvement gap junction proteins, connexins, remains elusive since they also suspected to promote cell invasion. In order better understand how connexins control glioma phenotype, we studied consequences inhibiting intrinsic expression major astrocytic connexin, Connexin43, in human U251...
ABSTRACT Adhesive interactions of cadherins induce crosstalk between adhesion complexes and the actin cytoskeleton, allowing strengthening adhesions cytoskeletal organization. The underlying mechanisms are not completely understood, microtubules (MTs) might be involved, as for integrin-mediated cell–extracellular-matrix adhesions. Therefore, we investigated relationship N-cadherin MTs by analyzing influence engagement on MT distribution dynamics. progressed less, with a lower elongation...
Abstract Physiologically relevant cell‐based models require engineered microenvironments which recapitulate the topographical, biochemical, and mechanical properties encountered in vivo. In this context, hydrogels are materials of choice. Here a light‐based toolbox is able to craft such microniches out common place materials. Extensive use benzophenone photoinitiators their interaction with oxygen achieves this. First, inhibition radicals harnessed photoprint hydrogel topographies. Then...
ABSTRACT Cell guidance by anchored molecules, or haptotaxis, is crucial in development, immunology and cancer. Adhesive adhesion well established for mesenchymal cells such as fibroblasts, whereas its existence remains unreported amoeboid that require less no order to migrate. We show that, vitro, human T lymphocytes develop adhesive haptotaxis mediated densities of integrin ligands expressed high endothelial venules. Moreover, orient towards increasing with VLA-4 integrins (also known...
Extracellular matrix (ECM) remodeling, degradation and glioma cell motility are critical aspects of glioblastoma multiforme (GBM). Despite being a rich source potential biomarkers targets for therapeutic advance, the dynamic changes occurring within extracellular environment that specific to GBM have yet be fully resolved. The gap junction protein connexin43 (Cx43) increases migration invasion in variety vitro vivo models. In this study, upregulation Cx43 C6 cells induced morphological...
3D cell culture aims at reconciliating the simplicity of in vitro models with human like properties encountered vivo. Soft permeable hydrogels have emerged as user-friendly materials to grow cells more physiological conditions. With intent on turning these homogeneous substrates into biomimetic templates, we introduce a generic solution compatible most biologically relevant and often frail materials. Here take control chemical environment driving radical reactions craft common gels patterned...
The formation of adhesive cell-cell contacts is based on the intrinsic binding properties between specific transmembrane ligand-receptor pairs. In neurons, synaptic adhesion molecules provide a physical linkage pre- and post-synaptic compartments, but strength dynamic these complexes in their actual membrane environments remain essentially unknown. To access such information, we developed versatile assay to measure affinity kinetics interactions, immobilization Fc-tagged ligands...
ABSTRACT Guidance of cells by molecules anchored on a substrate, known as haptotaxis, is arguably crucial in development, immunology and cancer, however the exact cues mechanisms driving cell orientation vivo are hardly identified. Adhesive haptotaxis has been described case mesenchymatous that develop strong pulling forces with their substrates orient via tug war mechanism – competition between cells’ edges. In amoeboid migrate minimal interaction existence adhesive remains unclear. Here,...
Abstract The shaping of a multicellular body and repair adult tissues require fine-tuning cell adhesion, mechanics intercellular transmission mechanical load. Adherens junctions (AJs) are the major by which cells sense exert force on each other. However, how AJs adapt to stress this adaptation contributes cell-cell cohesion eventually tissue-scale dynamics remains largely unknown. Here, analyzing tension-dependent recruitment vinculin, α-catenin F-actin as function stiffness, well GFP-tagged...
Glioblastomas (GBMs), grade IV malignant gliomas, are one of the deadliest types human cancer because their aggressive characteristics. Despite significant advances in genetics these tumors, how GBM cells invade healthy brain parenchyma is not well understood. Notably, it has been shown that peritumoral space via different routes; main interest this paper route along white matter tracts (WMTs). The interactions tumor with nervous cell components characterized. Herein, a method described...
ABSTRACT Adhesive interactions of cadherins induce crosstalk between adhesion complexes and the actin cytoskeleton, allowing strengthening adhesions cytoskeletal organization. The underlying mechanisms are not completely understood, microtubules (MTs) might be involved, as for integrin-mediated cell–extracellular-matrix adhesions. Therefore, we investigated relationship N-cadherin MTs by analyzing influence engagement on MT distribution dynamics. progressed less, with a lower elongation...