A5101791417- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Blood Coagulation and Thrombosis Mechanisms
- Lung Cancer Research Studies
- Myasthenia Gravis and Thymoma
- Lung Cancer Treatments and Mutations
- Mass Spectrometry Techniques and Applications
- Glioma Diagnosis and Treatment
- Protease and Inhibitor Mechanisms
- Ubiquitin and proteasome pathways
- Neuroblastoma Research and Treatments
- Parkinson's Disease Mechanisms and Treatments
- Ferroptosis and cancer prognosis
- Advancements in Photolithography Techniques
- Advanced Proteomics Techniques and Applications
- Integrated Circuits and Semiconductor Failure Analysis
- Cancer, Lipids, and Metabolism
- Functional Brain Connectivity Studies
- Analytical Chemistry and Chromatography
- Glycosylation and Glycoproteins Research
- Cancer Immunotherapy and Biomarkers
- Full-Duplex Wireless Communications
- Connexins and lens biology
- Vascular Tumors and Angiosarcomas
- Restless Legs Syndrome Research
- Fibroblast Growth Factor Research
Taiwan Semiconductor Manufacturing Company (Taiwan)
2024
Brandon University
2019-2024
Georgetown University Medical Center
2018-2022
Georgetown University
2018-2022
Chinese Academy of Sciences
2022
Dana-Farber Brigham Cancer Center
2022
Dana-Farber Cancer Institute
2022
Chang Gung University
2018-2021
Chiayi Chang Gung Memorial Hospital
2018-2021
Cancer Genetics (United States)
2021
To determine if motor restlessness in the Restless Legs Syndrome (RLS) shows a circadian rhythm with maximum at night, as previously found for subjective discomfort and periodic limb movements (PLMs), to correlate RLS peak intensity core temperature cycle. Subjects underwent two days of normally timed wakefulness sleep followed by night subsequent day deprivation. Activity was standardized through modified suggested immobilization tests (mSITs). The study conducted laboratory environment...
N-Linked glycans derived from human and bovine α1-acid glycoprotein, as well chicken egg white albumin, were analyzed by MALDI-TOF mass spectrometry using a novel MALDI matrix consisting of 2,5-dihydroxybenzoic acid (DHB) aniline. A significant increase in signal was observed for these oligosaccharides relative to the obtained when unmodified DHB used same set samples. The use aniline/DHB also led facile on-target derivatization via nonreductive amination, aniline found form stable Schiff...
The use of recombinant "bait" proteins to capture protein-binding partners, followed by identification protein interaction networks mass spectrometry (MS), has gained popularity and widespread acceptance. We have developed an approach using PDZ modules the membrane-associated guanylate kinase (MAGUK) zonula occludens-1 (ZO-1) pull-down screen for that interact with these via their domain binding motifs. Identification MS material was achieved a vacuum-based chromatography sample preparation...
Our study aimed to clarify the neuroimaging correlates of suicide attempt by comparing differences in functional magnetic resonance imaging (fMRI) among depressed attempters, patients without history, and healthy controls through comprehensive novel fMRI analyses methods same population. The association between depression severity aspects brain was also discussed. recruited 109 participants who were assigned three groups: 33 with (SA), 32 (NS), 44 (HC). All scanned using a 3 T MRI system...
It is estimated that at least one million people die by suicide every year, showing the importance of prevention and detection. In this study, an autoencoder machine learning model was employed to predict with suicidal ideation based on their structural brain imaging. The subjects in our generalized q-sampling imaging (GQI) dataset consisted three groups: 41 depressive patients (SI), 54 without thoughts (NS), 58 healthy controls (HC). GQI dataset, indices fractional anisotropy (GFA),...
Kallistatin is a heparin-binding serine proteinase inhibitor (serpin), which specifically inhibits human tissue kallikrein by forming covalent complex. The inhibitory activity of kallistatin blocked upon its binding to heparin. In this study we attempted locate the site using synthetic peptides derived from surface regions and site-directed mutagenesis basic residues in these regions. Two peptides, containing clusters positive-charged residues, one F helix other region encompassing H C2...
Platinum‐based chemotherapy has been the cornerstone treatment for small cell lung cancer (SCLC) decades, but no major progress made in past 20 years with regard to overcoming chemoresistance. As cycle checkpoint kinase 1 (Chk1) plays a key role DNA damage response chemotherapeutic drugs, we explored mechanisms of acquired drug resistance Chk1 inhibitor prexasertib SCLC. We established two SCLC lines and found that copy number, messengerRNA (mRNA) protein levels regulator Wee1 significantly...
There is an inverse correlation between systemic blood pressure and urinary kallikrein levels in humans hypertensive animal models, suggesting that the tissue kallikrein-kinin system plays important role regulation. In this study, we explored potential of human gene delivery on reduction spontaneously rats (SHR). The or cDNA was placed under control following promoters: metallothionein metal response-element (MRE-pHK), albumin (ALB-pHK), Rous sarcoma virus 3′ long terminal repeat (LTR)...
Kallistatin is a serpin with unique P1 Phe, which confers an excellent inhibitory specificity toward tissue kallikrein. In this study, we investigated the P3-P2-P1 residues (residues 386–388) of human kallistatin in determining kallikrein by site-directed mutagenesis and molecular modeling. Human mutants 19 different amino acid substitutions at each P1, P2, or P3 residue were created purified to compare their binding activity. Complex formation assay showed that Arg, Phe (wild type), Lys,...
Placental-specific protein 1 (PLAC1) is an X-linked trophoblast gene that re-expressed in several malignancies, including breast cancer, and therefore a potential biomarker to follow disease onset progression. Sera from 117 preoperative/pretreatment cancer patients 51 control subjects, those with fibrocystic disease, were analyzed for the presence of PLAC1 as well its expression by IHC tumor biopsies subset subjects. Serum levels exceeded mean plus one standard deviation (mean+SD) level...
Osimertinib is an effective first-line therapy option for EGFR-mutant NSCLC, but virtually all patients develop resistance. CRIPTO, through Src activation, has been implicated in resistance to EGFR tyrosine kinase inhibitor (EGFR-TKI) therapy. Dasatinib, a inhibitor, shown preclinical synergy with EGFR-TKI therapy.This single-arm phase I/II trial of osimertinib and dasatinib TKI-naïve advanced NSCLC (NCT02954523). A 3 + design was used the I establish recommended II dose (RP2D). 80 mg QD...
Kallistatin is a serine proteinase inhibitor (serpin) that specifically inhibits tissue kallikrein. The inhibitory activity of kallistatin abolished upon heparin binding. loop between the H helix and C2 sheet containing clusters basic amino acid residues has been identified as heparin-binding site. In this study, we investigated role in region kallikrein inhibition. mutants double Ala substitutions for these displayed 70–80% reduction association rate constants, indicating importance A...
Extracellular matrix (ECM) remodeling, degradation and glioma cell motility are critical aspects of glioblastoma multiforme (GBM). Despite being a rich source potential biomarkers targets for therapeutic advance, the dynamic changes occurring within extracellular environment that specific to GBM have yet be fully resolved. The gap junction protein connexin43 (Cx43) increases migration invasion in variety vitro vivo models. In this study, upregulation Cx43 C6 cells induced morphological...
Many drug candidates fail in clinical trials due to an incomplete understanding of how small-molecule perturbations affect cell phenotype. Cellular responses can be non-intuitive systems-level properties such as redundant pathways caused by co-activation multiple receptor tyrosine kinases. We therefore created a scalable algorithm, DIONESUS, based on partial least squares regression with variable selection reconstruct cellular signaling network human carcinoma line driven EGFR...
The separation of peptide mixtures from proteolytic cleavage is often necessary prior to mass spectrometry (MS) enhance sensitivity and mapping coverage. When buffers, salts, other higher abundance peptides/contaminants are present, competition for charge during the electrospray ionization matrix-assisted laser desorption/ionization (MALDI) processes can lead ion suppression targeted analyte(s). In this note, a simple reversed-phase microcolumn sample deposition device (Sep-Dep) described....
As technology continues to scale aggressively, Sub-Resolution Assist Features (SRAF) are becoming an increasingly key resolution enhancement technique (RET) maximize the process window enhancement. For past few generations, lithographers have chosen use a rules-based (RB-SRAF) or model-based (MB-SRAF) approach place assist features on design. The inverse lithography solution, which provides maximum entitlement, has always been out of reach for full-chip applications due its very high...
Abstract Protein phosphorylation is a type of posttranslational modification which plays an important role in cell regulation and signal transduction. Because its biological relevance, considerable amount interest has been paid to the development efficient techniques for phosphopeptide analysis. Although advances MS control have enabled high‐throughput discovery proteins from limited amounts sample, automated selection MS/MS precursor ions based on intensity alone can significantly hamper...