A5038091364- Liver Disease Diagnosis and Treatment
- Liver Disease and Transplantation
- Liver physiology and pathology
- Adenosine and Purinergic Signaling
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- Pancreatic function and diabetes
- Diet, Metabolism, and Disease
- Organ Transplantation Techniques and Outcomes
- RNA modifications and cancer
- Diet and metabolism studies
- Biochemical effects in animals
- Peroxisome Proliferator-Activated Receptors
- Epigenetics and DNA Methylation
- Genomics, phytochemicals, and oxidative stress
- Alcohol Consumption and Health Effects
- Cardiac Ischemia and Reperfusion
- ATP Synthase and ATPases Research
- Cancer, Hypoxia, and Metabolism
- Ion channel regulation and function
- Drug-Induced Hepatotoxicity and Protection
- Neuroscience and Neuropharmacology Research
- Neuroscience of respiration and sleep
- Amino Acid Enzymes and Metabolism
Universidad Nacional Autónoma de México
2016-2025
Instituto Nacional de Cardiología
2008-2022
University of Lisbon
2012
University of Ljubljana
2012
Texas Medical Center
1983
Baylor College of Medicine
1983
Experimental Medicine and Biology Institute
1975
Universidad Autónoma de la Ciudad de México
1972
Saint Louis University
1964
Acute myocardial infarction is the second cause of mortality in most countries, therefore, it important to know evolution and sequence physiological biochemical changes involved this pathology. This study attempts integrate these correlate them a long-term model (96 h) isoproterenol-induced cell damage rat. We achieved an infarct-like apex region left ventricle, occurring 12-24 h after isoproterenol administration. The lesion was defined by histological criteria, continuous telemetric ECG...
Abstract A basic question linked to differential patterns of gene expression is how cells reach different fates despite using the same DNA template. Since 5-hydroxymethylcytosine (5hmC) emerged as an intermediate metabolite in active demethylation, there have been increasing efforts elucidate its function a stable modification genome, including role establishing such tissue-specific expression. Recently we described TET1-mediated enrichment 5hmC on promoter region master regulator hepatocyte...
Abstract Mammalian cytosine DNA methylation (5mC) is associated with the integrity of genome and transcriptional status nuclear DNA. Due to technical limitations, it has been less clear if mitochondrial (mtDNA) methylated whether 5mC a regulatory role in this context. Here, we used bisulfite-independent single-molecule sequencing native human mouse study across different biological conditions. We first validated ability long-read nanopore detect CpG (5mCpG) non-CpG (5mCpH) context at...
Hepatocellular carcinoma (HCC) is the most common primary liver cancer, often diagnosed at advanced stages due to insufficient early screening and monitoring. MicroRNAs (miRNAs) are key regulators of gene expression potential biomarkers for cancer diagnosis. This study investigated diagnostic miRNAs in Extracellular Vesicles (EVs) from HCC. miRNA EVs was analyzed using HCC cell lines, circulating a Diethylnitrosamine (DEN)-induced tumor rat model, plasma samples patients. Receiver Operating...
Oxidative stress and its consequent lipid peroxidation (LP) exert harmful effects, which have been currently involved in the generation of carbon tetrachloride-induced cirrhosis. However, recent report that “physiological” LP can be associated with liver regeneration (LR) makes it necessary to discriminate between oxidative stress-induced LR-associated LP. In rats rendered cirrhotic by continuous CCl 4 administration for weeks, moderate cell necrosis fine fatty infiltration were found. The...
Cirrhosis is one of the most common causes mortality worldwide, because hepatic dysfunction constitutes a potentially lethal condition. Having demonstrated hepatoprotective effect adenosine against CCl 4 -induced cirrhosis, present study was aimed at assessing adenosine's on an already-established micronodular cirrhosis. Chronic administration (10 weeks) induced cirrhotic state, characterized by increased liver fibronectin and collagen types I III content, enhanced expression α-1 (I) mRNA,...
Adenosine administration was tested in rats with carbon tetrachloride-induced hepatic fibrosis and able to partially prevent the enlargement of liver spleen induced by toxin. This amelioration hepatomegaly accompanied a 50% reduction collagen deposition preservation content glycosaminoglycans. A stimulated collagenase activity is apparently mechanism for accumulation. These effects were associated striking improvement function. treatment did not modify late hepatotoxic effect tetrachloride;...
Regulatory T cells are considered key players in the prevention of allograft rejection transplanted patients. Belatacept, is an effective alternative to calcineurin inhibitors that appears preserve graft survival and function, however, impact this drug homeostasis Tregs patients remains controversial. Here we analyzed phenotype, function epigenetic status Treg-specific demethylated region (TSDR) FOXP3 circulating from long-term kidney transplant under Belatacept or Cyclosporine A treatment....
Idiopathic pulmonary fibrosis (IPF) is a devastating disease characterized by increased activation of fibroblasts/myofibroblasts. Previous reports have shown that IPF fibroblasts are resistant to apoptosis, but the mechanisms remain unclear. Since inhibition mitochondrial permeability transition pore (mPTP) has been implicated in resistance this study, we analyzed role function and mPTP on apoptosis under basal conditions after mitomycin C-induced apoptosis. We measured release cytochrome c,...
Mitochondrial bc 1 complex from yeast has 10 subunits, but only cytochrome b (Cyt ) subunit is encoded in the mitochondrial genome. Cyt eight transmembrane helices containing two hemes for electron transfer. Cbp3 and Cbp6 assist synthesis, together with Cbp4 induce hemylation. Subunits Qcr7/Qcr8 participate first steps of assembly, lack Qcr7 reduces synthesis through an assembly-feedback mechanism involving Cbp3/Cbp6. Because resides near carboxyl region, we wondered whether this region...
Obesity and alcohol consumption are risk factors for hepatic steatosis, both commonly coexist. Our objective was to evaluate the effect of ethanol acetaldehyde on primary hepatocytes obtained from mice fed two days with a high cholesterol (HC) diet. HC increased lipid content. diet sensitized toxic acetaldehyde. Cyp2E1 content diet, as well in those treated or acetaldehyde, while activity this enzyme determined microsomes all hepatocytes, CW. Oxidized proteins were cultures not toxins....
Hepatocellular carcinoma is one of the most common cancers, and approximately 80% develop from cirrhotic livers. We have previously shown that aspartate salt adenosine prevents reverses carbon tetrachloride–induced liver fibrosis in rats. Considering hepatoprotective role this derivative fibrogenesis, we were interested evaluating its effect a hepatocarcinogenesis model induced by diethylnitrosamine rats, where multinodular cancer preceded cirrhosis. Rats injected with for 12 weeks to induce...