A5006827322- Mitochondrial Function and Pathology
- RNA and protein synthesis mechanisms
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- ATP Synthase and ATPases Research
- RNA modifications and cancer
- Polyamine Metabolism and Applications
- Amino Acid Enzymes and Metabolism
- Plant biochemistry and biosynthesis
- Plant Genetic and Mutation Studies
- Agricultural and Food Sciences
- Microbial Metabolic Engineering and Bioproduction
- Agricultural and Food Production Studies
- GABA and Rice Research
- Business, Innovation, and Economy
- Molecular Biology Techniques and Applications
- Plant and soil sciences
- Metabolism and Genetic Disorders
- Plant Gene Expression Analysis
- Metalloenzymes and iron-sulfur proteins
- Genomics and Phylogenetic Studies
Universidad Nacional Autónoma de México
2007-2024
Instituto Nacional de Cardiología
2013-2014
Institute for Scientific and Technological Research
2004
Technological Institute of Celaya
2004
Center for Research and Advanced Studies of the National Polytechnic Institute
2002
Pet309 is a protein essential for respiratory growth. It involved in translation of the yeast mitochondrial COX1 gene, which encodes subunit I cytochrome c oxidase. also stabilization mRNA. Mutations similar human protein, Lrp130, are associated with Leigh syndrome, where oxidase activity affected. The sequence reveals presence at least seven pentatricopeptide repeats (PPRs) located tandem central portion protein. Proteins containing PPR motifs present mitochondria and chloroplasts general...
Mitochondrial synthesis of Cox1, the largest subunit cytochrome c oxidase complex, is controlled by Mss51 and Pet309, two mRNA-specific translational activators that act via COX1 mRNA 5′-UTR through an unknown mechanism. Pet309 belongs to pentatricopeptide repeat (PPR) protein family, which involved in RNA metabolism mitochondria chloroplasts, its sequence predicts at least 12 PPR motifs central portion protein. Deletion these selectively disrupted translation but not accumulation mRNA. We...
Mitochondrial bc 1 complex from yeast has 10 subunits, but only cytochrome b (Cyt ) subunit is encoded in the mitochondrial genome. Cyt eight transmembrane helices containing two hemes for electron transfer. Cbp3 and Cbp6 assist synthesis, together with Cbp4 induce hemylation. Subunits Qcr7/Qcr8 participate first steps of assembly, lack Qcr7 reduces synthesis through an assembly-feedback mechanism involving Cbp3/Cbp6. Because resides near carboxyl region, we wondered whether this region...
Synthesis of the largest cytochrome c oxidase (CcO) subunit, Cox1, on yeast mitochondrial ribosomes is coupled to assembly CcO. The translational activator Mss51 sequestered in early intermediate complexes by an interaction with Cox14 that depends presence newly synthesized Cox1. If CcO prevented, level available for activation reduced. We deleted C-terminal 11 or 15 residues Cox1 site-directed mutagenesis mtDNA. Although these deletions did not prevent respiratory growth yeast, they...
Message-specific translational regulation mechanisms shape the biogenesis of multimeric oxidative phosphorylation (OXPHOS) enzyme in mitochondria from yeast Saccharomyces cerevisiae. These mechanisms, driven mainly by action mRNA-specific activators, help to coordinate synthesis OXPHOS catalytic subunits mitoribosomes with both import their nucleus-encoded partners and assembly form holocomplexes. However, little is known regarding role that mitoribosome itself may play regulation. Here, we...
Baker´s yeast Saccharomyces cerevisiae has been widely used to understand mitochondrial biology for decades. This model provided knowledge about essential, conserved pathways among eukaryotes, and fungi or yeast-specific pathways. One of the many abilities S. is capacity manipulate genome, which so far only possible in unicellular algae Chlamydomonas reinhardtii. The biolistic transformation mitochondria allows us introduce site-directed mutations, make gene rearrangements, reporters. These...
We have designed and constructed four oligonucleotides corresponding to the most conserved regions of ornithine decarboxylases (ODC; EC 4.1.1.17) plant origin. These were used for amplification homologous fragments from several plants (Zea mays, Capsicum annuum, Sorghum bicolor, Phaseolus vulgaris, Carica papaya Daucus carota). The amplified cloned sequenced, revealing high homology other ODCs. Peptide sequences coded by these compared Clustal analyses. analyses identified location binding...
Objective: Develop a similar snack to marzipan, with higher nutritional quality by adding protein provide healthy consumption alternative. Design/methodology/approach: A made from blue corn flour, grasshopper, agave honey and cocoa was elaborated. The proportions of the aforementioned ingredients were modified in four different formulations. Acceptance tests conducted 140 individuals determine formulation highest degree approval laboratory carried out its quality. Results: In this research...
ABSTRACT Mitochondrial translation is a critical regulatory step in mitochondrial genome expression. In Saccharomyces cerevisiae , translational activators are believed to bind the 5’ UTRs of their target mRNAs position ribosome at start codon. Pet309 and Mss51 COX1 mRNA, which encodes subunit one cytochrome c oxidase. physically interacts with but no direct interaction its mRNA has been detected. Currently, mechanisms underlying activation or any other gene, remain poorly understood. To...
Mitochondria have the machinery to translate mRNAs produced inside organelle, however very little is understood about how mitochondrial ribosomes locate initiation codon. In yeast mitochondria a group of proteins known as translational activators recognize specific and are thought assist ribosome start codon, mechanism action these remains unknown. Pet309 activator for COX1 mRNA (1). It acts on 5′ untranslated region (UTR) induce translation. The protein predicted contain up 23...
Objective: Determine which agricultural practices are currently used by corn (Zea mays L.) growers in the municipality of Jilotepec, Estado de México.
 Design/methodology/approach: An individual survey composed both open and close-ended questions was applied to 93 producers from México, order obtain information about planting they carry out. From there, 10 were selected for on-site monitoring their plots. 
 Results: Maize conscious genetic cultural richness native maize. However,...
Abstract Mitochondrial bc 1 complex from yeast has ten subunits, but only Cytochrome b (Cyt ) subunit is encoded in the mitochondrial genome. Cyt eight transmembrane helices containing two hemes for electron transfer. Cbp3 and Cbp6 assist synthesis, together with Cbp4 induce hemylation. Subunits Qcr7/Qcr8 participate first steps of assembly, lack Qcr7 reduces synthesis through an assembly-feedback mechanism involving Cbp3/Cbp6. Since resides near carboxyl-region, we wondered whether this...