A5059102060- Renal Diseases and Glomerulopathies
- Chronic Kidney Disease and Diabetes
- Renal and related cancers
- T-cell and B-cell Immunology
- Genetic and Kidney Cyst Diseases
- Single-cell and spatial transcriptomics
- Sesquiterpenes and Asteraceae Studies
- COVID-19 Clinical Research Studies
- Immune Cell Function and Interaction
- Plant Toxicity and Pharmacological Properties
- Systemic Sclerosis and Related Diseases
- Cellular Mechanics and Interactions
- Pancreatic function and diabetes
- Systemic Lupus Erythematosus Research
- Macrophage Migration Inhibitory Factor
- Cancer-related molecular mechanisms research
- Cell Adhesion Molecules Research
- Erythrocyte Function and Pathophysiology
- Cancer Genomics and Diagnostics
- Vasculitis and related conditions
- Nuclear Receptors and Signaling
- Reproductive System and Pregnancy
- Immunotherapy and Immune Responses
- GDF15 and Related Biomarkers
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
University Medical Center Hamburg-Eppendorf
2018-2025
Universität Hamburg
2018-2025
Ludwig-Maximilians-Universität München
2007-2022
Swiss Integrative Center for Human Health
2008-2020
University of Zurich
2008-2020
LMU Klinikum
2008-2019
München Klinik
2019
Institut für Urheber- und Medienrecht
2018
Polyclinic Medical Center
2018
University Hospital of Zurich
2008-2016
Injury and loss of podocytes are leading factors glomerular disease renal failure. The postmitotic podocyte is the primary target for toxic, immune, metabolic, oxidant stress, but little known about how this cell type copes with stress. Recently, autophagy has been identified as a major pathway that delivers damaged proteins organelles to lysosomes in order maintain cellular homeostasis. Here we report exhibit an unusually high level constitutive autophagy. Podocyte-specific deletion...
Diabetic kidney disease is the leading cause of ESRD, but few biomarkers diabetic are available. This study used gas chromatography-mass spectrometry to quantify 94 urine metabolites in screening and validation cohorts patients with diabetes mellitus (DM) CKD(DM+CKD), DM without CKD (DM–CKD), healthy controls. Compared levels controls, 13 were significantly reduced DM+CKD (P≤0.001), 12 remained significant when compared DM–CKD cohort. Many differentially expressed water-soluble organic...
Chronic glomerular diseases, associated with renal failure and cardiovascular morbidity, represent a major health issue. However, they remain poorly understood. Here we have reported that tightly controlled mTOR activity was crucial to maintaining podocyte function, while dysregulation of facilitated diseases. Genetic deletion complex 1 (mTORC1) in mouse podocytes induced proteinuria progressive glomerulosclerosis. Furthermore, simultaneous both mTORC1 mTORC2 from aggravated the lesions,...
Extrapulmonary manifestations of COVID-19 have gained attention due to their links clinical outcomes and potential long-term sequelae1. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) displays tropism towards several organs, including the heart kidney. Whether it also directly affects liver has been debated2,3. Here we provide clinical, histopathological, molecular bioinformatic evidence for hepatic SARS-CoV-2. We find that injury, indicated by a high frequency abnormal function...
The endoplasmic reticulum (ER) is an important site for protein folding and becomes "stressed" when its capacity to fold proteins overwhelmed. In response, "unfolded response" (UPR) genes are induced, increasing the proteins; if response insufficient, then apoptosis ensues. For investigation of whether proteinuria hyperglycemia induce ER stress in renal epithelial cells, microarray data from biopsies established diabetic nephropathy (DN) were analyzed. Expression UPR was significantly...
Diabetic nephropathy (DN) is a frequent complication in patients with diabetes. Although the majority of DN models and human studies have focused on glomeruli, tubulointerstitial damage major feature an important predictor renal dysfunction. This study sought to investigate molecular markers pathogenic pathways interstitium DN. Microdissected compartments from biopsies established control kidneys were subjected expression profiling. Analysis candidate genes, potentially involved basis common...
Lupus nephritis (LN) is a serious manifestation of systemic lupus erythematosus. Therapeutic studies in mouse LN models do not always predict outcomes human therapeutic trials, raising concerns about the relevance these preclinical models. In this study, we used an unbiased transcriptional network approach to define, molecular terms, similarities and differences among three LN. Genome-wide gene-expression networks were generated using natural language processing automated promoter analysis...
Apoptosis of podocytes is considered critical in the pathogenesis diabetic nephropathy (DN). Free fatty acids (FFAs) are critically involved diabetes mellitus type 2, particular regulation pancreatic β cell survival. The objectives this study were to elucidate role palmitic acid, palmitoleic, and oleic acid podocyte death endoplasmic reticulum (ER) stress. We show that increases death, both apoptosis necrosis podocytes, a dose time-dependent fashion. Palmitic induces ER stress, leading an...
<h3>Objectives</h3> To characterise renal tissue metabolic pathway gene expression in different forms of glomerulonephritis. <h3>Methods</h3> Patients with nephrotic syndrome (NS), antineutrophil cytoplasmic antibody-associated vasculitis (AAV), systemic lupus erythematosus (SLE) and healthy living donors (LD) were studied. Clinically indicated biopsies obtained at time diagnosis microdissected into glomerular tubulointerstitial compartments. Microarray-derived differential 88 genes...
Although it is well established that microbial infections predispose to autoimmune diseases, the underlying mechanisms remain poorly understood. After infection, tissue-resident memory T (TRM) cells persist in peripheral organs and provide immune protection against reinfection. However, whether TRM participate responses unrelated primary such as inflammation, unknown. By using high-dimensional single-cell analysis, we identified CD4+ with a TH17 signature (termed TRM17 cells) kidneys of...
The cellular origins of glomerulosclerosis involve activation parietal epithelial cells (PECs) and progressive podocyte depletion. While mammalian target rapamycin-mediated (mTOR-mediated) hypertrophy is recognized as an important signaling pathway in the context glomerular disease, role a compensatory mechanism preventing PEC remains poorly understood. In this study, we show that mTOR activation-related genes were both upregulated intercorrelated biopsies from patients with focal segmental...
Significance Statement The ability to produce glucose from nonhexose precursors is a main metabolic function of renal proximal tubule (PT) cells. PT cells adapt metabolically during CKD, but little known about gluconeogenesis in chronically injured Our study demonstrates the progressive loss enzymes animal models and CKD patients parallel global change pathway expression activation injury pathways. This alteration not only due has systemic repercussions on lactate levels experimental human...
Diabetic nephropathy (DN) is the leading cause of end-stage renal disease, and histopathologic glomerular lesions are among earliest structural alterations DN. However, signaling pathways that initiate these incompletely understood.To delineate cellular molecular basis for DN initiation, we performed single-cell bulk RNA sequencing cells from type 2 diabetes mice (BTBR ob/ob) at early stage DN.Analysis differentially expressed genes revealed glucose-independent responses in cell types. The...
Kidney diseases impart a vast burden on affected individuals and the overall health care system. Progressive loss of renal parenchymal cells functional decline following injury are often observed. Notch-1 -2 receptors crucially involved in nephron development contribute to inflammatory kidney diseases. We specifically determined participation receptor Notch-3 tubulointerstitial responses. Here we show by heat map analyses that transcripts up-regulated human A similar response was...
Endothelial dysfunction is a central pathomechanism in diabetes-associated complications. We hypothesized pathogenic role this of cathepsin S (Cat-S), cysteine protease that degrades elastic fibers and activates the protease-activated receptor-2 (PAR2) on endothelial cells. found injection mice with recombinant Cat-S induced albuminuria glomerular cell injury PAR2-dependent manner. In vivo microscopy confirmed for intrinsic Cat-S/PAR2 ischemia-induced microvascular permeability. vitro...