A5073790325- Cancer Cells and Metastasis
- Cancer Genomics and Diagnostics
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- Cancer-related Molecular Pathways
- Cancer, Hypoxia, and Metabolism
- Molecular Biology Techniques and Applications
- Diagnosis and treatment of tuberculosis
- Tuberculosis Research and Epidemiology
- Nanocomposite Films for Food Packaging
- Infectious Diseases and Tuberculosis
- Genetic factors in colorectal cancer
- Gut microbiota and health
- Cancer, Lipids, and Metabolism
- Mathematical Biology Tumor Growth
- Wnt/β-catenin signaling in development and cancer
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
- Pineapple and bromelain studies
- RNA modifications and cancer
- RNA Interference and Gene Delivery
- Bacillus and Francisella bacterial research
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Chronic Lymphocytic Leukemia Research
Translational Research Institute
2017-2024
Queensland University of Technology
2017-2024
The University of Queensland
2020-2022
Lovely Professional University
2014-2018
Academy of Scientific and Innovative Research
2015
Institute of Genomics and Integrative Biology
2015
Dynamic interconversions between transitional epithelial and mesenchymal states underpin the plasticity (EMP) seen in some carcinoma cell systems. We have delineated subpopulations existing within PMC42-LA breast cancer line by their EpCAM expression. These purified but phenotypically plastic states, EpCAMHigh (epithelial) EpCAMLow (mesenchymal), ability to regain phenotypic equilibrium of parental population (i.e., 80% 20% mesenchymal) over time, although rate reversion direction...
Abstract Extrapulmonary manifestations constitute 15 to 20% of tuberculosis cases, with lymph node (LNTB) as the most common form infection. However, diagnosis and treatment advances are hindered by lack understanding LNTB biology. To identify host response, Mycobacterium infected nodes from patients were studied means transcriptomics quantitative proteomics analyses. The selected targets obtained comparative analyses validated PCR immunohistochemistry. This approach provided expression data...
Fomites are a well-known source of microbial infections and previous studies have provided insights into the sojourning microbiome fomites from various sources. Paper currency notes one most commonly exchanged objects its potential to transmit pathogenic organisms has been well recognized. Approaches identify associated with paper largely limited culture dependent approaches. Subsequent portrayed use 16S ribosomal RNA based approaches which taxonomical distribution microbiome. However,...
Epithelial–mesenchymal plasticity (EMP), encompassing epithelial–mesenchymal transition (EMT) and mesenchymal–epithelial (MET), are considered critical events for cancer metastasis. We investigated chromosomal heterogeneity instability (CIN) profiles of two sister PMC42 breast (BC) cell lines to assess the relationship between their karyotypes EMP phenotypic plasticity. Karyotyping by GTG banding exome sequencing were aligned with SWATH quantitative proteomics existing RNA-sequencing data...
Phenotypic heterogeneity is a hallmark of aggressive cancer behaviour and clinical challenge. Despite much characterisation this at multi-omics level in many cancers, we have limited understanding how emerges spontaneously an isogenic cell population. Some longitudinal observations dynamics epithelial-mesenchymal heterogeneity, canonical example phenotypic offered us opportunities to quantify the rates switching that may drive such heterogeneity. Here, offer mathematical modeling framework...
Cancer cell populations comprise phenotypes distributed among the epithelial-mesenchymal (E-M) spectrum. However, it remains unclear which population-level processes give rise to observed experimental distribution and dynamical changes in E-M heterogeneity, including (1) differential growth, (2) cell-state switching, (3) population density-dependent growth or state-transition rates. Here, we analyze necessity of these three explaining dynamics distributions as PMC42-LA HCC38 breast cancer...
Breast cancer (BCa) is a major global health challenge, characterized by chromosomal instability (CIN) and subsequent acquisition of extensive somatic copy number alterations (CNAs). CNAs including amplifications deletions, significantly influence intra-tumor heterogeneity the tumor microenvironment (TME). Among these, loss chromosome 13q14.2 emerges as considerable factor in BCa pathogenesis treatment responses. We provide evidence that this genomic alteration under positive selective...
Abstract Breast cancer (BCa) is a major global health challenge. The BCa genome often carries extensive somatic copy number alterations (CNAs), including gains/amplifications and losses/deletions. These CNAs significantly affect tumor development, drug response patient survival. However, how individual contribute mostly elusive. We identified loss of chromosome 13q14.2 as key CNA in BCa, occurring up to 63% patients, depending on the subtype, correlating with poor Through multi-omics vitro...
Background: Breast cancer (BC) is a heterogeneous disease for which the commonly used chemotherapeutic agents primarily include anthracyclines (doxorubicin, epirubicin), microtubule inhibitors (paclitaxel, docetaxel, eribulin), and alkylating (cyclophosphamide). While these drugs can be highly effective, metastatic tumours are frequently refractory to treatment or become resistant upon tumour relapse. Methods: We undertook cell polarity/epithelial mesenchymal plasticity (EMP)-enriched short...
The epithelial-mesenchymal (E/M) hybrid state has emerged as an important mediator of the elements cancer progression facilitated by epithelial mesenchymal plasticity (EMP). We review here evidence for presence and prognostic potential E/M in carcinoma, modelling predictions validations studies to demonstrate stabilised intermediates along spectrum EMP, computational approaches characterising quantifying EMP phenotypes, with particular attention emerging realm single-cell through RNA...
<title>Abstract</title> Breast cancer (BCa) is a major global health challenge, characterized by chromosomal instability (CIN) and subsequent acquisition of extensive somatic copy number alterations (CNAs). CNAs including amplifications deletions, significantly influence intra-tumor heterogeneity the tumor microenvironment (TME). Among these, loss chromosome 13q14.2 emerges as considerable factor in BCa pathogenesis treatment responses. We provide evidence that this genomic alteration under...
Cancer is a heterogenous disease, with multiple cellular subpopulations present within single tumour mass that differ genetically and morphologically, thus respond differently to chemotherapeutics. Epithelial-to-Mesenchymal transition (EMT) has been shown play role in heterogeneity. Single-cell sequencing critical identify cell-type-specific transcriptomic differences multiplexing methods increasing experimental scope reduced cost. Cell hashing barcoded antibodies commonly used multiplex...
The consortia of micro organisms obtained from contaminated food (Milkfed Verka Kheer) was effectively tested against antibacterial agents, i.e. Sodium benzoate, grinded grape fruit seed powder GSP, Lysozyme and EDTA by agar diffusion method. Bacterial inhibition combinations using two levels each the three factors (EDTA, benzoate Lysozyme) without AM films evaluated liquid incubation agents were selected according to their permissibility standards. Statistical analysis experimental data for...
Epithelial-mesenchymal plasticity (EMP) is a hallmark of cancer. By enabling cells to shift between different morphological and functional states, EMP promotes invasion, metastasis therapy resistance. We report that near-diploid non-cancerous human epithelial lung spontaneously along the spectrum without genetic changes. Strikingly, more than half single cell-derived clones adopt mesenchymal morphology. independently characterise epithelial-like mesenchymal-like clones. Mesenchymal lose...
Epithelial–mesenchymal transition (EMT) is a reversible and dynamic biological process in which epithelial cells acquire mesenchymal characteristics including enhanced stemness migratory ability. EMT can facilitate cancer metastasis known driver of cellular resistance to common chemotherapeutic drugs, such as docetaxel. Current practices docetaxel treatment promote increase the chance tumor recurrence resistance, calling for new approaches treatment. Here we show that prolonged at sub-IC50...