A5018870356- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Salmonella and Campylobacter epidemiology
- Diagnosis and treatment of tuberculosis
- Dental Radiography and Imaging
- Infectious Diseases and Tuberculosis
- Oropharyngeal Anatomy and Pathologies
- Dental Implant Techniques and Outcomes
- dental development and anomalies
- Diet and metabolism studies
- Dietary Effects on Health
- Child Nutrition and Feeding Issues
- Laser Applications in Dentistry and Medicine
- Scientific and Engineering Research Topics
- Blood Coagulation and Thrombosis Mechanisms
- Signaling Pathways in Disease
- Bacterial Identification and Susceptibility Testing
- Dermatology and Skin Diseases
- Cancer therapeutics and mechanisms
- Bacterial Genetics and Biotechnology
- Microbial Natural Products and Biosynthesis
- Metabolomics and Mass Spectrometry Studies
South Dakota State University
2019-2024
Dr. R. Ahmed Dental College and Hospital
2024
University of South Dakota
2022
Burdwan Medical College & Hospital
2021
Indian Institute of Science Education and Research, Bhopal
2017-2021
Institute of Genomics and Integrative Biology
2013-2019
Council of Scientific and Industrial Research
2014
Metagenomic studies carried out in the past decade have led to an enhanced understanding of gut microbiome human health; however, Indian has not been well explored. We analyzed 110 healthy individuals from two distinct locations (North-Central and Southern) India using multi-omics approaches, including 16S rRNA gene amplicon sequencing, whole-genome shotgun metagenomic metabolomic profiling fecal serum samples.The catalogue established this study emphasizes uniqueness comparison other...
Recently, dysbiosis in the human gut microbiome and shifts relative abundances of several bacterial species have been recognized as important factors colorectal cancer (CRC). However, these studies carried out mainly developed countries where CRC has a high incidence, it is unclear whether host-microbiome relationships deduced from can be generalized to global population. To test if documented associations between are conserved distinct context, we performed metagenomic metabolomic...
Tuberculosis (TB) is primarily associated with decline in immune health status. As gut microbiome (GM) implicated the regulation of host immunity and metabolism, here we investigate GM alteration TB patients by 16S rRNA gene whole-genome shotgun sequencing. The study group constituted pulmonary their healthy household contacts as controls (HCs). Significant microbial taxonomic functional capacity was observed active compared to HCs. We that Prevotella Bifidobacterium abundance were HCs,...
Abstract The abundance and diversity of host-associated Prevotella species have a profound impact on human health. To investigate the composition, diversity, functional roles in gut, population-wide analysis was carried out 586 healthy samples from western non-western populations including largest Indian cohort comprising 200 samples, 189 Inflammatory Bowel Disease populations. A higher copri enriched complex plant polysaccharides metabolizing enzymes, particularly pullulanase containing...
The human gut microbiota is a complex community comprising hundreds of species, with few present in high abundance and the vast majority low abundance. biological functions effects these low-abundant species on their hosts are not yet fully understood. In this study, we assembled bacterial consortium (SC-4) consisting B. paravirosa, C. comes, M. indica, A. butyriciproducens, which low-abundant, short-chain fatty acid (SCFA)-producing bacteria isolated from healthy gut, tested its effect host...
A complex microbial community in the gut may prevent colonization of enteric pathogens such as
A major function of the gut microbiota is to provide colonization resistance, wherein pathogens are inhibited or suppressed below infectious levels. However, fraction required for resistance remains unclear. We used culturomics isolate a culture collection comprising 1,590 isolates belonging 102 species. This represents 34.57% taxonomic diversity and 70% functional capacity, as estimated by metagenomic sequencing fecal samples culture. Using whole-genome sequencing, we characterized species...
Abstract Extrapulmonary manifestations constitute 15 to 20% of tuberculosis cases, with lymph node (LNTB) as the most common form infection. However, diagnosis and treatment advances are hindered by lack understanding LNTB biology. To identify host response, Mycobacterium infected nodes from patients were studied means transcriptomics quantitative proteomics analyses. The selected targets obtained comparative analyses validated PCR immunohistochemistry. This approach provided expression data...
Mycobacterium tuberculosis modulates expression of various metabolism-related genes to adapt in the adverse host environment. The gene coding for M. S-adenosylhomocysteine hydrolase (Mtb-SahH) is essential optimal growth and protein product involved intermediary metabolism. However, relevance SahH mycobacterial physiology unknown. In this study, we analyze role Mtb-SahH regulating homocysteine concentration surrogate smegmatis. catalyzes reversible hydrolysis adenosine demonstrate that...
PrkC is a conserved Ser/Thr protein kinase encoded in Bacillus anthracis genome. shown to be important for B. pathogenesis, but little known about its other functions and phosphorylated substrates. Systemic analyses indicate the compelling role of phosphorylating multiple substrates, including essential chaperone GroEL. Through mass spectrometry, we identified that phosphorylates GroEL on six threonine residues are distributed three canonical regions. Phosphorylation facilitates...
Cyclophilins are prolyl isomerases with multitude of functions in different cellular processes and pathological conditions. Cyclophilin A (PpiA) Mycobacterium tuberculosis is secreted during infection intraphagosomal niche. However, our understanding about the evolutionary origin, secretory mechanism or interactome M. PpiA limited. This study demonstrates through phylogenetic structural analyses that has more proximity to human cyclophilins than prokaryotic counterparts. We report a unique...
Background Mycobacterium ulcerans, the causative agent of Buruli ulcer in humans, is unique among members genus due to presence virulence determinant megaplasmid pMUM001. This plasmid encodes multiple virulence-associated genes, including mup011, which an uncharacterized Ser/Thr protein kinase (STPK) PknQ. Methodology/Principal Findings In this study, we have characterized PknQ and explored its interaction with MupFHA (Mup018c), a FHA domain containing also encoded by was found interact...
Abstract A complex microbial community in the gut generally prevent colonization of enteric pathogens such as Salmonella . Because high complexity, several species or combination can confer resistance. To gain a better understanding resistance against enterica , we isolated library 1,300 bacterial strains from feral chicken microbiota which represented total 51 species. Using co-culture assay, screened representative this and identified 30 that inhibited Typhimurium. improve inhibition...
Bacterial alternative sigma factors are mostly regulated by a partner-switching mechanism. Regulation of the virulence-associated factor SigF Mycobacterium tuberculosis has been an area intrigue, with having more predicted regulators than other in this organism. Rv1364c is one such regulator, mechanism which confounded presence both anti-sigma and antagonist functions single polypeptide. Using protein binding phosphorylation assays, we demonstrate that domain mediates autophosphorylation its...
This report describes the understudied co-occurrence of temporomandibular joint ankylosis (TMJA) and extrahepatic portal vein obstruction (EHPVO), exploring a shared pathway involving hypercoagulability. TMJA is an acquired pathology where surfaces fuse, causing restricted mouth opening facial asymmetry. Globally, prevalent among 1.5 to 5 patients/million, with higher incidence in developing countries. While trauma infections often cause TMJA, pathogenesis remains unclear many cases. Recent...
Mandibular canines, also known as the “cornerstone” of dental arch, are important factors for arch stability, masticatory function, and maintaining natural facial expressions aesthetics. Due to their eruption pattern sequence, canines prone impactions with a 20 times higher frequency maxillary than mandibular canines. Bilateral permanent rare occurrences low incidence 0.12%. Migration teeth is frequently documented ectopia. However, pre-eruptive migration across midline an unusual occurrence...
Commensal gut bacteria are key contributors to the resilience against pathogen invasion. This is exemplified by success of fecal microbiota transplantation in treating recurrent Clostridioides difficile infection. Yet, characteristics communities that can confer colonization resistance and underlying mechanisms remain largely unknown. Here we use a synthetic community 14 commensal uncover inter-species interactions metabolic pathways underpinning emergent C. We challenged this as well...
Abstract The human gut microbiota is a complex community comprising hundreds of species, with few present in high abundance and the vast majority low abundance. biological functions effects these low-abundant species on their hosts are not yet fully understood. In this study, we assembled bacterial consortium (SC-4) consisting B. paravirosa, C. comes, M. indica , A. butyriciproducens which low-abundant, short-chain fatty acid (SCFA)-producing bacteria isolated from healthy gut, tested its...
Background: Healing unbinds a well choreographed array of cellular, physiologic, biochemical, and molecular processes directed toward restoring the integrity functional capacity damaged structures. The rate degree healing vary among individuals but is greatest during first few months or years after tooth loss. Evidence suggests that alveolar bone ridge resorption may be prevented, initially by reconstructive surgical techniques using several materials which are employed at time extraction....