A5071984855- Malaria Research and Control
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Immune Cell Function and Interaction
- Tuberculosis Research and Epidemiology
- Complement system in diseases
- Bacterial Genetics and Biotechnology
- Invertebrate Immune Response Mechanisms
- Mycobacterium research and diagnosis
- Bacillus and Francisella bacterial research
- Vector-Borne Animal Diseases
- Enzyme Structure and Function
- HIV Research and Treatment
- Biochemical and Molecular Research
- T-cell and B-cell Immunology
- Insect and Pesticide Research
- Toxin Mechanisms and Immunotoxins
- SARS-CoV-2 and COVID-19 Research
- Cancer therapeutics and mechanisms
- Bacterial biofilms and quorum sensing
- Diabetes Management and Research
- COVID-19 diagnosis using AI
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
National Institutes of Health
2016-2024
Yale University
2020-2024
National Institute of Allergy and Infectious Diseases
2016-2024
National Heart Lung and Blood Institute
2024
VA Nebraska Western Iowa Health Care System
2024
Saint Joseph Hospital
2023
St. Joseph Hospital
2023
St. Joseph Medical Center in Tacoma
2023
Trinity Hospital of Augusta
2023
Institute of Genomics and Integrative Biology
2009-2020
An Ixodes scapularis saliva mRNA vaccine induces tick resistance and prevents Borrelia burgdorferi infection in guinea pigs.
Lyme disease is the most common vector-borne infectious in United States, part because a vaccine against it not currently available for humans. We propose utilizing lipid nanoparticle-encapsulated nucleoside-modified mRNA (mRNA-LNP) platform to generate like successful clinical vaccines SARS-CoV-2. Of antigens expressed by Borrelia burgdorferi, causative agent of disease, outer surface protein A (OspA) promising candidate development. have designed and synthesized an OspA-encoding mRNA-LNP...
Tuberculosis (TB) is primarily associated with decline in immune health status. As gut microbiome (GM) implicated the regulation of host immunity and metabolism, here we investigate GM alteration TB patients by 16S rRNA gene whole-genome shotgun sequencing. The study group constituted pulmonary their healthy household contacts as controls (HCs). Significant microbial taxonomic functional capacity was observed active compared to HCs. We that Prevotella Bifidobacterium abundance were HCs,...
Antibodies acquired naturally through repeated exposure to Plasmodium falciparum are essential in the control of blood-stage malaria. Antibody-dependent functions may include neutralization parasite–host interactions, complement activation, and activation Fc receptor functions. A role antibody-dependent cellular cytotoxicity (ADCC) by natural killer (NK) cells protection from malaria has not been established. Here we show that IgG isolated adults living a malaria-endemic region activated...
In malaria-naïve children and adults, Plasmodium falciparum -infected red blood cells ( Pf -iRBCs) trigger fever other symptoms of systemic inflammation. However, in endemic areas where individuals experience repeated infections over many years, the risk -iRBC-triggered inflammatory decreases with cumulative exposure. The molecular mechanisms underlying these clinical observations remain unclear. Age-stratified analyses uninfected, asymptomatic Malian before malaria season revealed that...
How antibodies naturally acquired during Plasmodium falciparum infection provide clinical immunity to blood-stage malaria is unclear. We studied the function of natural killer (NK) cells in people living a malaria-endemic region Mali. Multi-parameter flow cytometry revealed high proportion adaptive NK cells, which are defined by loss transcription factor PLZF and Fc receptor γ-chain. Adaptive dominated antibody-dependent cellular cytotoxicity responses, their frequency within total...
During protein synthesis, translation elongation factor Tu (Ef-Tu) is responsible for the selection and binding of cognate aminoacyl-tRNA to acceptor site on ribosome. The activity Ef-Tu dependent its interaction with GTP. Posttranslational modifications, such as phosphorylation, are known regulate in several prokaryotes. Although a study Mycobacterium tuberculosis phosphoproteome showed be phosphorylated, role phosphorylation regulation has not been studied. In this report, we show that M....
ABSTRACT HU, a widely conserved bacterial histone-like protein, regulates many genes, including those involved in stress response and virulence. Whereas ample data are available on HU-DNA communication, the knowledge how HU perceives signal transmit it to DNA remains limited. In this study, we identify HupB, homolog of human pathogen Mycobacterium tuberculosis , as component serine/threonine protein kinase (STPK) signaling. HupB is extracted its native state from exponentially growing cells...
Abstract Extrapulmonary manifestations constitute 15 to 20% of tuberculosis cases, with lymph node (LNTB) as the most common form infection. However, diagnosis and treatment advances are hindered by lack understanding LNTB biology. To identify host response, Mycobacterium infected nodes from patients were studied means transcriptomics quantitative proteomics analyses. The selected targets obtained comparative analyses validated PCR immunohistochemistry. This approach provided expression data...
Interleukin 15 (IL-15) is an essential cytokine for the survival and proliferation of natural killer (NK) cells. IL-15 activates signaling by β common γ (γc) chain heterodimer IL-2 receptor through trans-presentation cells expressing bound to α (IL-15Rα). We show here that membrane-associated IL-15Rα-IL-15 complexes are transferred from presenting NK trans-endocytosis contribute phosphorylation ribosomal protein S6 cell proliferation. interaction with soluble or surface-bound complex...
Gene-edited mosquitoes lacking a gamma-interferon-inducible lysosomal thiol reductase-like protein, namely (mosGILT
Mycobacterium tuberculosis profoundly exploits protein phosphorylation events carried out by serine/threonine kinases (STPKs) for its survival and pathogenicity. Forkhead-associated domains (FHA), the phosphorylation-responsive modules, have emerged as prominent players in STPK mediated signaling. In this study, we demonstrate association of previously uncharacterized FHA domain-containing Rv0019c with cognate PknB. The consequent is shown to be dependent on conserved residues domain...
Mycobacterium tuberculosis modulates expression of various metabolism-related genes to adapt in the adverse host environment. The gene coding for M. S-adenosylhomocysteine hydrolase (Mtb-SahH) is essential optimal growth and protein product involved intermediary metabolism. However, relevance SahH mycobacterial physiology unknown. In this study, we analyze role Mtb-SahH regulating homocysteine concentration surrogate smegmatis. catalyzes reversible hydrolysis adenosine demonstrate that...