A5029205217- Trypanosoma species research and implications
- Insect symbiosis and bacterial influences
- Vector-borne infectious diseases
- Research on Leishmaniasis Studies
- Invertebrate Immune Response Mechanisms
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Parasite Biology and Host Interactions
- Lysosomal Storage Disorders Research
- Insect and Pesticide Research
- Parasites and Host Interactions
- Mollusks and Parasites Studies
- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Viral Infectious Diseases and Gene Expression in Insects
- Fungal and yeast genetics research
- Vector-Borne Animal Diseases
- Study of Mite Species
- Machine Learning in Bioinformatics
- Lipid metabolism and biosynthesis
- Skin Protection and Aging
- Cardiomyopathy and Myosin Studies
- Plant and soil sciences
- Metabolism and Genetic Disorders
- Extracellular vesicles in disease
University of Maryland, Baltimore
2021-2024
The University of Texas at El Paso
2016-2023
Universitat de Barcelona
2020
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol
2020
Centro Nacional de Epidemiología
2020
Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública
2020
Instituto de Salud Carlos III
2020
Barcelona Institute for Global Health
2020
Universidade Federal do Triângulo Mineiro
2014-2018
Current treatment for Chagas disease with the only available drugs, benznidazole or nifurtimox, has substantial limitations, including long duration and safety tolerability concerns. We aimed to evaluate efficacy of new monotherapy regimens combinations fosravuconazole, in disease.We did a double-blind, double-dummy, phase 2, multicentre, randomised trial three outpatient units Bolivia. Adults aged 18-50 years chronic indeterminate disease, confirmed by serological testing positive...
The ability to undergo morphological changes during adaptation distinct environments is exploited by Candida albicans and has a direct impact on biofilm formation virulence. Morphogenesis controlled diversity of stimuli, including osmotic stress, pH, starvation, presence serum, microbial components, among others.
Abstract Uncovering the complexity of systems in non-model organisms is critical for understanding arthropod immunology. Prior efforts have mostly focused on Dipteran insects, which only account a subset existing species nature. Here we use and develop advanced techniques to describe immune cells (hemocytes) from clinically relevant tick Ixodes scapularis at single-cell resolution. We observe molecular alterations hemocytes upon feeding infection with either Lyme disease spirochete Borrelia...
ABSTRACT Trypanosoma cruzi , the aetiologic agent of Chagas disease, releases vesicles containing a wide range surface molecules known to affect host immunological responses and cellular infectivity. Here, we compared secretome two distinct strains (Y YuYu) T. which were previously shown differentially modulate innate acquired immune responses. Tissue culture‐derived trypomastigotes both secreted extracellular (EVs), as demonstrated by electron scanning microscopy. EVs purified exclusion...
The immune deficiency (IMD) pathway directs host defense in arthropods upon bacterial infection. In Pancrustacea, peptidoglycan recognition proteins sense microbial moieties and initiate nuclear factor-κB-driven responses. Proteins that elicit the IMD non-insect remain elusive. Here, we show an Ixodes scapularis homolog of croquemort (Crq), a CD36-like protein, promotes activation tick pathway. Crq exhibits plasma membrane localization binds lipid agonist 1-palmitoyl-2-oleoyl- sn...
Dendritic cells (DCs) are professional antigen-presenting with vital roles in the activation of host immunity. Ticks bloodsucking arthropods that secrete bioactive compounds immunomodulatory properties via their saliva. It is known some tick species modulate biology DCs different intensities; however, studies on Amblyomma cajennense, Cayenne tick, have not yet been performed, although this considered one most capable modulating immune responses hosts. Engorged female ticks were stimulated...
Chagas disease is caused by the protozoan Trypanosoma cruzi and characterized cardiac, gastrointestinal, nervous system disorders. Although much about pathophysiological process of already known, influence parasite burden on inflammatory progression remains uncertain. We used an acute experimental model to evaluate effect T. intestinal lesions assessed correlations between load inflammation injury at 7 14 days post-infection. Low (3 × 102), medium 103), high 104) loads were generated...
Dendritic cells (DCs) are major immune components, and depending on how these modulated, the protective host response changes drastically. Trypanosoma cruzi is a parasite with high genetic variability modulates DCs by interfering their capacity for antigen recognition, migration, maturation. Despite recent efforts, association between T. I (TcI) TcII populations unknown. Herein, it was demonstrated that AQ1.7 MUTUM TcI strains present low rates of invasion bone marrow-derived DCs, whereas...
Triatomines are hematophagous arthropod vectors of Trypanosoma cruzi, the causative agent Chagas Disease. Panstrongylus lignarius, also known as herreri, is considered one most versatile triatomines because it can parasitize different hosts, found in habitats and countries, has sylvatic, peridomestic domestic behavior a very important vector disease, especially Peru. Molecules produced secreted by salivary glands fat body adaptational value for because, among other functions, they subvert...
Chagas disease is emerging in countries to which it not endemic. Biomarkers for earlier therapeutic response assessment patients with chronic are needed. We profiled plasma-derived extracellular vesicles from a heart transplant patient and showed the potential of this approach discovering such biomarkers.
Triatomines are blood-sucking vectors of Trypanosoma cruzi, the causative agent Chagas disease. During feeding, triatomines surpass skin host response through biomolecules present in their saliva. Dendritic cells (DCs) play a crucial role induction protection to aggressive agents, including arthropods. Here, we evaluated if salivary components from different genera evade immunity by modulating biology and function LPS- or T. cruzi-stimulated DCs. Saliva Panstrongylus lignarius, Meccus...
Uncovering the complexity of systems in non-model organisms is critical for understanding arthropod immunology. Prior efforts have mostly focused on Dipteran insects, which only account a subset existing species nature. Here, we describe immune cells or hemocytes from clinically relevant tick
Wound healing has been extensively studied through the lens of inflammatory disorders and cancer, but limited attention given to hematophagy arthropod-borne diseases. Hematophagous ectoparasites, including ticks, subvert wound response maintain prolonged attachment facilitate blood-feeding. Here, we unveil a strategy by which extracellular vesicles (EVs) ensure blood-feeding arthropod survival in three medically relevant tick species. We demonstrate single cell RNA sequencing murine genetics...
Abstract The ability to undergo morphological changes during adaptation distinct environments is exploited by Candida albicans and has a direct impact on virulence. In this study, we investigated the influence of fungal extracellular vesicles (EVs) yeast growth, biofilm formation, morphogenesis in C. . Addition EVs ( Ca EVs) culture medium positively affected growth. Using crystal violet staining scanning electron microscopy (SEM), demonstrated that inhibited formation vitro By time-lapse...
Rhodnius neglectus is a potential vector of Trypanosoma cruzi (Tc), the causative agent Chagas disease. The salivary glands (SGs) and intestine (INT) are actively required during blood feeding. saliva from SGs injected into vertebrate host, modulating immune responses favoring feeding for INT digestion. Tc infection significantly alters physiology these tissues; however, studies that assess this still scarce. This study aimed to gain better understanding global transcriptional expression...
The data presented here were obtained from the saliva of three triatominae, Rhodnius prolixus, Triatoma lecticularia and Panstrongylus herreri Montandon et al. study, doi:10.1016/j.ibmb.2016.02.009 [3]. These spectra generated by mass spectrometry proteins observed through analysis 2-D electrophoretic profiles. analyzed according to UniProt code, protein name, group, isoelectric point molecular weight, profile, referring UniProt, volume percentage spot number peptides percent coverage found...
Light emitting diode (LED) therapy has been proposed as an option for the treatment of many skin inflammatory processes. Dendritic cells (DCs) are important that participate in initiation and activation immunity. The modulation these by LED could explain much its effects.Thus, aim this study was to examine effects at 460 ± 20 nm on cytokine production expression surface markers DCs.DCs were obtained from mouse bone marrow-derived dendritic (BMDCs). applied giving a fluence 3.3, 8.2, or 16.5...
Trypanosomatids are a diverse group of uniflagellate protozoan parasites that include globally relevant pathogens such as Trypanosoma cruzi, the causative agent Chagas disease. Trypanosomes lack fatty acid synthase system typically used for de novo (FA) synthesis in other eukaryotes. Instead, these microbes have evolved modular FA elongase (ELO) comprised individual ELO enzymes (ELO1-4) can operate processively to generate long chain- and very chain-FAs. The importance ELO's maintaining...
Panstrongylus herreri is a main Chagas disease vector, and its success as vector stems from ability to establish domiciliated colonies; we aimed explore biology reproduction.The average amount of blood ingested the time beginning meal production feces were recorded.Females exhibited higher ingestion rate than males, but similar defecation times frequencies observed.Despite detected decrease in oviposition rates, P. herreri's potential environments other Amazon forest cannot be discounted.