A5051914576- Insect symbiosis and bacterial influences
- Mosquito-borne diseases and control
- Entomopathogenic Microorganisms in Pest Control
- Dengue and Mosquito Control Research
- Invertebrate Immune Response Mechanisms
- Malaria Research and Control
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Aquaculture disease management and microbiota
- Vector-Borne Animal Diseases
- Environmental DNA in Biodiversity Studies
- Vibrio bacteria research studies
- Parasitic infections in humans and animals
- Parasitic Infections and Diagnostics
- Parasite Biology and Host Interactions
- Complement system in diseases
- Cephalopods and Marine Biology
- Extracellular vesicles in disease
University of Maryland, Baltimore
2018-2024
ORCID
2020
Pennsylvania State University
2015
Abstract Uncovering the complexity of systems in non-model organisms is critical for understanding arthropod immunology. Prior efforts have mostly focused on Dipteran insects, which only account a subset existing species nature. Here we use and develop advanced techniques to describe immune cells (hemocytes) from clinically relevant tick Ixodes scapularis at single-cell resolution. We observe molecular alterations hemocytes upon feeding infection with either Lyme disease spirochete Borrelia...
Significance A better understanding of the molecular mechanisms underlying development Plasmodium parasites in Anopheles mosquitoes would facilitate malaria vaccines and novel strategies to interrupt disease transmission. We characterized, at single-cell level, gene expression profiles sporozoites from rodent parasite berghei , throughout their upon salivation. Our analyses reveal heterogeneity among isolated same anatomical location, suggesting that is asynchronous mosquitoes. In addition,...
Several of the most devastating human diseases are caused by eukaryotic parasites transmitted arthropod vectors or through food and water contamination. These pathogens only represent a fraction all unicellular eukaryotes helminths that present in environment many uncharacterized organisms might have subtle but pervasive effects on health, including modifying microbiome where they reside. Unfortunately, while we modern molecular tools to characterize bacterial and, lesser extent, fungal...
Most Plasmodium vivax infections contain genetically distinct parasites, but the consequences of this polyclonality on development asexual their sexual differentiation, and transmission remain unknown. We describe Saimiri monkeys with two strains P. analyses 80,024 parasites characterized by single cell RNA sequencing individually genotyped. In our model, consecutive inoculations fail to establish polyclonal infections. By contrast, simultaneous lead sustained infections, although without...
Bacteria often use transcription factors to regulate the expression of metabolic genes in accordance available nutrients. NagC is a repressor conserved among γ-proteobacteria that regulates enzymes involved metabolism N-acetyl-glucosamine (GlcNAc). The polymeric form GlcNAc, known as chitin, has been shown play roles chemotactic signaling and nutrition within light organ symbiosis established between marine bacterium Vibrio fischeri Hawaiian squid Euprymna scolopes. Here, we investigate...
Abstract Background The commensal microbiota of mosquitoes impacts their development, immunity, and competency, could provide a target for alternative entomological control approaches. However, despite the importance mosquito/microbiota interactions, little is known about relative contribution endogenous exogenous factors in shaping bacterial communities mosquitoes. Methods We used high-throughput sequencing-based assay to characterize composition diversity 665 individual field-caught...
Uncovering the complexity of systems in non-model organisms is critical for understanding arthropod immunology. Prior efforts have mostly focused on Dipteran insects, which only account a subset existing species nature. Here, we describe immune cells or hemocytes from clinically relevant tick
Wound healing has been extensively studied through the lens of inflammatory disorders and cancer, but limited attention given to hematophagy arthropod-borne diseases. Hematophagous ectoparasites, including ticks, subvert wound response maintain prolonged attachment facilitate blood-feeding. Here, we unveil a strategy by which extracellular vesicles (EVs) ensure blood-feeding arthropod survival in three medically relevant tick species. We demonstrate single cell RNA sequencing murine genetics...
ABSTRACT Vector-borne pathogens cause many human infectious diseases and are responsible for high mortality morbidity throughout the world. They can also livestock epidemics with dramatic social economic consequences. Due to its costs, vector-borne disease surveillance is often limited current threats, investigation of emerging typically occurs after reports clinical cases. Here, we use high-throughput sequencing detect identify a wide range parasites viruses carried by mosquitoes from...
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Abstract Background The commensal microbiota of mosquitoes impacts their development, immunity, and competency, could provide a target for alternative entomological control approaches. However, despite the importance mosquito/microbiota interactions, little is known about relative contribution genetic non-genetic factors in shaping bacterial communities mosquitoes. Methods We used high-throughput sequencing based assay to characterize composition diversity 665 individual field-caught...
Most Plasmodium vivax infections contain genetically distinct parasites, but the consequences of this polyclonality on development asexual their sexual differentiation, and transmission remain unknown. We describe Saimiri monkeys with two strains P. analyses 117,350 parasites characterized by single cell RNA sequencing individually genotyped. show that consecutive inoculations fail to establish polyclonal infections. By contrast, simultaneous lead sustained infections, although without...
Vector-borne pathogens cause many human infectious diseases and are responsible for high mortality morbidity throughout the world. They can also livestock epidemics with dramatic social economic consequences. Due to costs, vector-borne disease surveillance is often limited current threats, investigation of emerging typically occur after reports clinical cases. Here, we use high-throughput sequencing detect identify a wide range parasites viruses carried by mosquitoes from Cambodia, Guinea,...