A5038017223- Mosquito-borne diseases and control
- Malaria Research and Control
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Insect Resistance and Genetics
- Insect and Arachnid Ecology and Behavior
- Insect and Pesticide Research
- Plant and animal studies
- Aquaculture disease management and microbiota
- interferon and immune responses
- Viral Infections and Vectors
- Neurobiology and Insect Physiology Research
- Insect Pest Control Strategies
- Cytomegalovirus and herpesvirus research
- Hepatitis C virus research
- Vector-borne infectious diseases
- Studies on Chitinases and Chitosanases
- T-cell and B-cell Immunology
- Insect Utilization and Effects
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Mycobacterium research and diagnosis
- Viral Infectious Diseases and Gene Expression in Insects
- Trypanosoma species research and implications
- Vector-Borne Animal Diseases
Sanaria
2025
National Institute of Allergy and Infectious Diseases
2021-2024
National Institutes of Health
2021-2024
Government of the United States of America
2023
Universidade Federal de Viçosa
2011-2020
Anopheles mating is initiated by the swarming of males at dusk followed females flying into swarm. Here, we show that mosquito and are coordinately guided clock genes, light, temperature. Transcriptome analysis shows up-regulation genes period (per) timeless (tim) in head field-caught coluzzii males. Knockdown per tim expression affects gambiae s.s. stephensi male laboratory, it reduces An. under semifield conditions. Light temperature affect mating, possibly modulating and/or expression....
Significance A better understanding of the molecular mechanisms underlying development Plasmodium parasites in Anopheles mosquitoes would facilitate malaria vaccines and novel strategies to interrupt disease transmission. We characterized, at single-cell level, gene expression profiles sporozoites from rodent parasite berghei , throughout their upon salivation. Our analyses reveal heterogeneity among isolated same anatomical location, suggesting that is asynchronous mosquitoes. In addition,...
The yellow fever mosquito Aedes aegypti is essentially a container-inhabiting species that closely associated with urban areas. This vector of human pathogens, including dengue and viruses, its control paramount importance for disease prevention. Insecticide use against juvenile stages (i.e. larvae pupae) growing in importance, particularly due to the ever-growing problems resistance adult-targeted insecticides safety concerns regarding such dwellings. However, insecticide effects on insects...
The life cycle of Plasmodium falciparum is characterized by complex regulatory changes that allow adaptation the parasites to different environmental conditions, which are especially pronounced during transmission between mammalian host and insect vector. Previous studies have shown P. uses three types ribosomal RNAs (rRNA A-, S1- S2-types) at stages its cycle. We used Oxford Nanopore Technologies (ONT) direct RNA sequencing investigate dynamics rRNA usage throughout parasite's...
Plasmodium sporozoites, the stage that initiates a malaria infection, must invade mosquito salivary glands (SGs) before transmitting to vertebrate host. However, effects of sporozoite invasion on gland physiology and saliva composition remain largely unexplored. We examined impact infection Anopheles gambiae using high-resolution proteomics, gene expression, morphological analysis. The data revealed differential expression various proteins, including enrichment humoral proteins in infected...
The mosquito Aedes aegypti is the main vector of Dengue, Chikungunya, Zika, and yellow fever viruses, which are responsible for high human morbidity mortality. fight against these pathogens mainly based on control insect with use insecticides. Among insecticides, spinosad bioinsecticide efficient A. larvae may be an alternative control. Here, we investigate sublethal effects during midgut metamorphosis females its cumulative blood acquisition capacity fecundity in adults. We studied because...
Abstract Background Successful mating of female mosquitoes typically occurs once, with the male sperm being stored in spermatheca for every subsequent oviposition event. The is responsible maintenance, nourishment, and protection against damage during storage. Aedes aegypti a major vector arboviruses, including Yellow Fever, Dengue, Chikungunya, Zika. Vector control difficult due to this mosquito high reproductive capacity. Results Following comparative RNA-seq analyses spermathecae obtained...
Upon infecting its vertebrate host, the malaria parasite initially invades liver where it undergoes massive replication, whilst remaining clinically silent. The coordination of host responses across complex tissue during infection remains unexplored. Here, we perform spatial transcriptomics in combination with single-nuclei RNA sequencing over multiple time points to delineate host-pathogen interactions Plasmodium berghei-infected tissues. Our data reveals significant changes gene expression...
Plasmodium parasites must migrate across proteinaceous matrices to infect the mosquito and vertebrate hosts. Plasmin, a mammalian serine protease, degrades extracellular matrix proteins allowing cell migration through tissues. We report that gametes recruit human plasminogen their surface where it is processed into plasmin by corecruited activators. Inhibition of activation arrests parasite development early during sexual reproduction, before ookinete formation. show increased fibrinogen...
Abstract In mammals, the serine protease plasmin degrades extracellular proteins during blood clot removal, tissue remodeling, and cell migration. The zymogen plasminogen is activated into by two proteases: tissue-type activator (tPA) urokinase-type (uPA), a process regulated inhibitor 1 (PAI-1), that specifically inhibits tPA uPA. Plasmodium gametes sporozoites use uPA to activate parasite-bound matrices, facilitating parasite motility in mosquito mammalian host. Furthermore, inhibition of...
The vectorial capacity of Aedes aegypti is directly influenced by its high reproductive output. Nevertheless, females are restricted to a single mating event, sufficient acquire enough sperm fertilize lifetime supply eggs. How Ae. able maintain viable spermatozoa remains mystery. Male stored within either two spermathecae that in consist one large and smaller organs each. In addition, each organ divided into reservoir, duct glandular portions. Many aspects the morphology spermatheca virgin...
Abstract The mosquito Stegomyia aegypti (= Aedes ) ( D iptera: C ulicidae) is a vector for the dengue and yellow fever viruses. As blood digestion occurs in midgut, this organ constitutes route of entry many pathogens. effects insecticide imidacloprid on survival St. were investigated sub‐lethal midgut development determined. Third instar larvae exposed to different concentrations (0.15, 1.5, 3.0, 6.0 15.0 p.p.m.) was monitored every 24 h 10 days. Midguts from imidacloprid‐treated insects at...
Plasmodium vivax infections often consist of heterogenous populations parasites at different developmental stages and with distinct transcriptional profiles, which complicates gene expression analyses. The advent single cell RNA sequencing (scRNA-seq) enabled disentangling this complexity has provided robust stage-specific characterization expression. However, scRNA-seq information is typically derived from the end each mRNA molecule (usually 3'-end) therefore fails to capture diversity in...
Abstract The vectorial capacity of mosquitoes is related to the reproductive output, and dependent on ability male spermatozoa survive within inseminated female. Mosquito females mate once, immediately after mating, are transferred maintained in ectodermic spermatheca. spermathecae culicines, especially yellowfever mosquito Aedes (Stegomyia) aegypti (L.), have been characterized detail. In contrast, not much known about this organ anophelines. Here, morphology spermatheca saltwater-tolerant...
Most Plasmodium vivax infections contain genetically distinct parasites, but the consequences of this polyclonality on development asexual their sexual differentiation, and transmission remain unknown. We describe Saimiri monkeys with two strains P. analyses 80,024 parasites characterized by single cell RNA sequencing individually genotyped. In our model, consecutive inoculations fail to establish polyclonal infections. By contrast, simultaneous lead sustained infections, although without...
Several studies have focused on understanding the biochemistry and morphology of fat body hematophagous mosquito Aedes aegypti (L.) (Diptera: Culicidae). In contrast, few studies, if any, morphological characters in other mosquitoes, especially non-hematophagous taxa such as culicid Toxorhynchites. Larvae Toxorhynchites prey upon larvae species are used vector control. We investigated aspects trophocytes, including morphometric analyses lipid droplets, protein granules nuclei, during...
Infection of tsetse fly with trypanosome parasites could be influenced by its ability to locate vertebrate host(s) in the wild. Generally, antennae insects are known bear chemo-sensory organs (sensilla), which used for host search among other functions. In order exploit potentials tsetse-search behavior, knowledge sensilla types on is desirable. line this, dorsal and ventral surfaces Glossina palpalis tachinoides (Westwood) were examined under scanning electron microscope. Results showed...
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Abstract Background Aedes and Anopheles mosquitoes are responsible for tremendous global health burdens from their transmission of pathogens causing malaria, lymphatic filariasis, dengue, yellow fever. Innovative vector control strategies will help to reduce the prevalence these diseases. Mass rearing research support presently depends on meals vertebrate blood, which is subject acquisition, handling, storage issues. Various blood-free replacements have been formulated mosquitoes, but none...
Most Plasmodium vivax infections contain genetically distinct parasites, but the consequences of this polyclonality on development asexual their sexual differentiation, and transmission remain unknown. We describe Saimiri monkeys with two strains P. analyses 117,350 parasites characterized by single cell RNA sequencing individually genotyped. show that consecutive inoculations fail to establish polyclonal infections. By contrast, simultaneous lead sustained infections, although without...
ABSTRACT Upon infecting its vertebrate host, the malaria parasite initially invades liver where it undergoes massive replication, whilst remaining clinically silent. The spatial coordination of factors regulating immune responses and metabolic zonation during infection, in true tissue context, remains unexplored. Here, we perform transcriptomics combination with single-nuclei RNA-sequencing (snRNA-seq) over multiple time points infection to delineate transcriptional programs host-pathogen...