A5021002884- Mosquito-borne diseases and control
- Malaria Research and Control
- Insect symbiosis and bacterial influences
- Insect behavior and control techniques
- Insect Pest Control Strategies
- Insect Resistance and Genetics
- Insect and Pesticide Research
- Insect Utilization and Effects
- Insect and Arachnid Ecology and Behavior
- Viral Infections and Vectors
- Plant and animal studies
- Neurobiology and Insect Physiology Research
- Dengue and Mosquito Control Research
- African Botany and Ecology Studies
- Vector-Borne Animal Diseases
- Trypanosoma species research and implications
- Insect-Plant Interactions and Control
- Animal and Plant Science Education
- Evolution and Genetic Dynamics
- Viral Infections and Outbreaks Research
- CRISPR and Genetic Engineering
- Forensic Entomology and Diptera Studies
- Allelopathy and phytotoxic interactions
- Radioactive contamination and transfer
- Agriculture and Rural Development Research
Institut de Recherche en Sciences de la Santé
2016-2025
International Atomic Energy Agency
2016-2025
Centre Muraz
2013-2023
Modèles Insectes de l'Immunité Innée
2022
Weatherford College
2020
Université Joseph Ki-Zerbo
2020
FAO/IAEA Agriculture and Biotechnology Laboratories
2020
Nazi Boni University
2020
National Institute of Allergy and Infectious Diseases
2017
National Institutes of Health
2017
Anopheles mating is initiated by the swarming of males at dusk followed females flying into swarm. Here, we show that mosquito and are coordinately guided clock genes, light, temperature. Transcriptome analysis shows up-regulation genes period (per) timeless (tim) in head field-caught coluzzii males. Knockdown per tim expression affects gambiae s.s. stephensi male laboratory, it reduces An. under semifield conditions. Light temperature affect mating, possibly modulating and/or expression....
The sterile insect technique is based on the overflooding of a target population with released males inducing sterility in wild female population. It has proven to be effective against several pest species agricultural and veterinary importance under development for Aedes mosquitoes. Here, we show that release at high male ratios may also impact through mating harassment. Under laboratory conditions, above 50 1 reduce longevity mosquitoes by reducing their feeding success. controlled blood...
Malaria control programs are being jeopardized by the spread of insecticide resistance in mosquito vector populations. It has been estimated that could lead to an additional 120000 deaths per year, and interfere with prospects for sustained or feasibility achieving malaria elimination. Another complication development management strategies is that, addition resistance, behavior evolves a manner diminishes impact LLINs IRS. Mosquitoes may circumvent LLIN IRS through preferential feeding...
Abstract Background The sterile insect technique (SIT) for use against mosquitoes consists of several steps including the production target species in large numbers, separation males and females, sterilization males, packing, transport release at site. sterility is basis technique; this, efficient standardized irradiation methods are needed to ensure that required level reliably reproducibly achieved. While reports have found certain biological factors, handling varying procedures can alter...
Anopheles gambiae mates in flight. Males gather at stationary places sunset and compete for incoming females. Factors that account male mating success are not known but critical the future of any genetic control strategy. The current study explored variations nutritional reserves (sugars, glycogen, lipids, proteins) wild-caught swarming resting males evaluated effect body size wing symmetry on success. Our results showed glycogen sugar mobilized consume proportionally 5.9-fold as much energy...
The Sterile Insect Technique (SIT) is presently being tested to control dengue in several countries. SIT aims cause the decline of target insect population through release a sufficient number sterilized male insects. This induces sterility female population, as females that mate with males produce no offspring. Male insects are use ionizing irradiation. study aimed evaluate variable parameters may affect irradiation mosquito pupae.
Effective mosquito population suppression has been repeatedly demonstrated in field trials through the release of male mosquitoes to induce sterile mating with wild females using incompatible insect technique (IIT), (SIT), or their combination. However, upscaling these techniques requires a highly efficient and scalable approach for sex separation mass-reared minimize unintentional females, which can lead either replacement biting nuisance, major bottleneck up now. Here, we report successful...
Background Monitoring the efficacy of sterile insect technique (SIT) programs, it is desirable to discriminate between wild and tsetse males captured in monitoring traps. Currently, this primarily achieved by marking with fluorescent dye powder before release, identifying them using a fluorescence camera and/or microscope. However, accuracy method limited due defective flies contaminated few particles Molecular techniques have been developed doubtful flies, but they are expensive for endemic...
Effective tools to prevent mosquito bites are essential for malaria control. The Lehmann Funnel Entry Trap (LFET), a window screen proven effective in reducing density, was tested its large-scale impact on vector control and community acceptance. A total of 1313 traps were deployed Vallée du Kou 3 (VK3), with 12 randomly selected detailed evaluation against untrapped houses 5 (VK5). Traps placed windows doors blocked by curtains. From July October, mosquitoes collected nine days per month...
Background: Genetic control tools, such as the sterile insect technique (SIT) and genetically modified mosquitoes (GMMs), require releasing males comparable to their wild counterparts. Ensuring that released do not exhibit higher insecticide resistance is critical. This study assessed phenotypic characteristics susceptibility of key dengue malaria vector species. Methods: Phenotypic deltamethrin (0.05%) was tested in two-to-five-day-old male female Aedes aegypti (Linnaeus, 1762) (Borabora...
<title>Abstract</title> <italic>Aedes</italic> mosquitoes are the vectors of dengue and other arboviruses, which threaten billions people world-wide. The boosted sterile insect technique (boosted SIT) is a version SIT where irradiated male also transmit biocide to wild females. We describe three field trials: one against <italic>Aedes aegypti</italic> in La Reunion, two albopictus</italic> Spain, all using pyriproxyfen as biocide. relative density adults comparison control sites, decreased...
Insect mass-rearing is an essential requirement for the sterile insect technique. Production at a large scale requires development of standardized rearing procedures to produce good quality males able compete with wild mate females. Three sets experiments (using trays placed on table, whole tray-rack system, and climate-controlled chambers) have been conducted aiming determine optimal water temperature number eggs aliquot into each larval tray achieve highest production pupae. No difference...
Insect symbionts are major manipulators of host's behavior. Their effect on parameters such as fecundity, male mating competitiveness, and biological quality in general, can have a influence the effectiveness sterile insect technique (SIT). SIT is currently being developed applied against human disease vectors, including Ae. albopictus, an environment-friendly method population suppression, therefore there renewed interest both characterization gut microbiota their exploitation artificial...
The success of the sterile insect technique (SIT) relies on achievement high levels sterility and mating factory-reared males thus their biological quality, which can be enhanced by reduction stress factors encountered during rearing, handling, irradiation procedures. consistent requires reliable standard protocols. Additionally, mosquito adults require immobilization prior to, to increase processing efficiency avoid physical damage caused movement in restricted space. Common methods for...
An area-wide integrated pest management strategy with a sterile insect technique (SIT) component requires radiation source for the sterilisation of male insects. Self-contained gamma irradiators, which were exclusively used in past SIT programmes, are now facing increasing constraints and challenges due to stringent regulations. As potential alternative, new generation high output X-ray irradiators have been proposed. The feasibility using was assessed by comparing effects both gamma- on...
AbstractCrimean-Congo hemorrhagic fever is a tick-borne disease caused by the arbovirus Crimean-Congo virus (CCHFV, family Bunyaviridae, genus Nairovirus). CCHFV can cause severe with high-case fatality rates in humans. has wide geographic range and been described around 30 countries Middle East, Asia, Europe, Africa including Mali neighboring countries. To date, little known about prevalence of Mali. Here, using banked bovine serum samples from across country, we describe results...
Understanding the factors that account for male mating competitiveness is critical to development of sterile insect technique (SIT). Here, effects partial sterilization with 90 Gy radiation on sexual Anopheles coluzzii allowed mate in different ratios untreated males have been assessed. Moreover, was compared between one versus two days contact females.Sterile and four six age were released large cages (~1.75 sq m) females similar at following males: virgin females: 100:100:100, 300:100:100,...
Radiation induced sterility is the basis of Sterile Insect Technique, by which a target insect pest population suppressed releasing artificially reared sterile males species in overflooding numbers over site. In order for to be high biological quality, effective standard irradiation protocols are required. Following studies investigating effects mosquito pupae water versus air, there need investigate oxy-regulatory behavior better understand consequences hypoxic normoxic conditions.
Successful implementation of the sterile insect technique (SIT) against Aedes aegypti and albopictus relies on maintaining a consistent release high-quality males. Affordable, rapid, practical quality control tools based male’s flight ability (ability to escape from device) may contribute meeting this requirement. Therefore, study aims standardize use original FAO/IAEA rapid test device (FTD) (version 1.0), while improving handling conditions reducing device’s overall cost by assessing...