Dengue virus infections are a serious public health problem worldwide. Aedes aegypti is the primary vector of dengue in Cuba. As there no vaccine or specific treatment, control efforts directed to reduction mosquito populations. The indiscriminate use insecticides can lead adverse effects on ecosystems, including human health. sterile insect technique species-specific and environment-friendly method population based release large numbers insects, ideally males only. success this for...

Tsetse flies (Genus: Glossina) are the sole cyclical vectors of African trypanosomoses. Despite their economic and public health impacts in sub-Saharan Africa, it has been decades since latest distribution maps at continental level were produced. The Food Agriculture Organization United Nations is trying to address this shortcoming through Atlas tsetse animal trypanosomosis.For component Atlas, a geospatial database being assembled which comprises information on trypanosomal infection...

Genetic control methods of mosquito vectors malaria, dengue, yellow fever, and Zika are becoming increasingly popular due to the limitations other techniques such as use insecticides. The sterile insect technique is an effective genetic method manage populations. However, it crucial release mosquitoes by air ensure homogeneous coverage, especially in large areas. Here, we report a fully automated adult system operated from uncrewed aerial vehicle or drone. Our system, developed tested...

Abstract Chemical control of disease vectoring mosquitoes Aedes albopictus and aegypti is costly, unsustainable, increasingly ineffective due to the spread insecticide resistance. The Sterile Insect Technique a valuable alternative but limited by slow, error-prone, wasteful sex-separation methods. Here, we present four Genetic Sexing Strains (two for each species) based on fluorescence markers linked m M sex loci, allowing isolation transgenic males. Furthermore, demonstrate how combining...

Several mosquito population suppression strategies based on the rearing and release of sterile males have provided promising results. However, lack an efficient male selection method has hampered expansion these approaches into large-scale operational programmes. Currently, most programmes targeting Aedes mosquitoes rely sorting methods sexual size dimorphism (SSD) at pupal stage. The currently available not been developed biometric analysis, there is therefore potential for improvement. We...

Abstract Background African animal trypanosomosis (AAT), transmitted by tsetse flies, is arguably the main disease constraint to integrated crop-livestock agriculture in sub-Saharan Africa, and heads of state governments adopted a resolution rid continent this scourge. In order sustainably reduce or eliminate burden AAT, progressive evidence-based approach needed, which must hinge on harmonized, spatially explicit information occurrence AAT its vectors. Methods A digital repository was...

Aedes aegypti is the primary vector of arthropod-borne viruses including dengue, chikungunya and Zika. Vector population control methods are reviving to impede disease transmission. An efficient sex separation for male-only releases crucial area-wide mosquito suppression strategies. Here, we report on construction two genetic sexing strains using red- white-eye colour mutations as selectable markers. Quality analysis showed that Red-eye (GSS) better more genetically stable than White-eye...

Abstract Background In the 1980s and 1990s, great strides were taken towards elimination of tsetse animal African trypanosomiasis (AAT) in Zimbabwe. However, advances recent years have been limited. Previously freed areas at risk reinvasion, disease tsetse-infested remains a constraint to food security. As part ongoing control activities, monitoring AAT is performed regularly main risk. centralized digital archive missing. To fill this gap, spatially explicit, national-level database (i.e....

Aedes albopictus and aegypti are the main vectors of arboviral diseases such as dengue, Zika chikungunya viruses. About a third world population is currently at risk contracting Aedes-borne epidemics. In recent years, A. has drastically increased its distribution in many countries. absence efficient mosquito vector control methods, sterile insect technique (SIT) presented very promising environment-friendly tool. The Agriculture Department Valencian Region promoting an ongoing pilot project...

Successful implementation of the sterile insect technique (SIT) against Aedes aegypti and albopictus relies on maintaining a consistent release high-quality males. Affordable, rapid, practical quality control tools based male’s flight ability (ability to escape from device) may contribute meeting this requirement. Therefore, study aims standardize use original FAO/IAEA rapid test device (FTD) (version 1.0), while improving handling conditions reducing device’s overall cost by assessing...

Abstract Autocidal control of the Mediterranean fruit fly Ceratitis capitata (Wiedemann) is an environmentally friendly and safe practice employed successfully worldwide. A key component in Sterile Insect Technique (SIT) to release sterile males that can compete with wild nature. Exposure ginger root oil (GRO) form aromatherapy prior has been shown significantly improve male competitiveness performance. However, alternatives GRO exposure are desired (i) reduce personnel costs handling‐time...

Introduction The widespread emergence of resistance to insecticides used control adult Aedes mosquitoes has made traditional strategies inadequate for the reduction various vector populations. Therefore, complementary methods, such as Sterile Insect Technique, are needed enhance existing efforts. technique relies on rearing and release large numbers sterile males, development efficient standardized mass-rearing procedures tools is essential its application against medically important...

Dengue virus infections are a serious public health problem worldwide. Aedes aegypti is the primary vector of dengue in Cuba. Since there no vaccine or specific treatment, control efforts directed to reduce mosquito populations. The indiscriminate use pesticides can lead increase insecticide resistance as well adverse effects on human health. sterile insect technique species-specific and environmental friendly method based release large numbers males. success this sustainable agricultural...

In Subsaharan Africa, tsetse flies (genus Glossina) are vectors of trypanosomes causing Human African Trypanosomiasis (HAT) and Animal Trypanosomosis (AAT). Some foci HAT persist in Southern Chad, where a program control was started against the local vector Glossina fuscipes Mandoul focus 2014, Maro 2018. Flies were also sampled 2018 Timbéri Dokoutou. We analyzed population genetics G. from four tsetse-infested zones. The trapping samples characterized by strong female biased sex-ratio,...

Abstract Area-wide sterile insect technique (SIT) programs assume that offspring reduction of the target population correlates with mating success males released. However, there is a lack monitoring tools to prove these in real-time. Field-cage tests were conducted under environmental conditions Mediterranean coast Spain estimate: (a) Vienna-8 (V8) Ceratitis capitata using molecular markers and (b) their efficacy reduce C. populations six release ratios wild females V8 (1:0:0, 1:1:0, 1:1:1,...

The sterile insect technique (SIT) application, as an alternative tool for conventional mosquito control methods, has recently gained prominence. Nevertheless, some SIT components require further development, such protocols under large-scale conditions, focusing on packing and shipping mosquitoes, considering transporting time. Immobilization of Aedes aegypti males was tested at temperatures 4, 7, 10, 14 °C, each temperature assessed 60, 90, 120 min. recovery after 24 h also studied. Chilled...

Tsetse flies are the cyclical vectors of African trypanosomes and one several methods to manage this vector is sterile insect technique (SIT). The ability determine sex tsetse pupae with objective separate sexes before adult emergence has been a major goal for decades management programmes an SIT component. females develop faster pharate inside melanise 1-2 days males. This earlier melanisation can be detected by infrared cameras through pupal shell, newly developed Near InfraRed Pupae Sex...

The success of an area-wide sterile insect technique (SIT) programme against Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) relies on the mating males in field. Limited information is available about effectiveness achieving mates with wild females and how these matings contribute to reducing populations. To this end, firstly a competition test was performed laboratory different release ratios (1:1:0, 1:1:1, 1:1:5, 1:1:10 1:1:20 for females:wild males:sterile VIENNA-8 respectively)...

Tsetse flies (genus Glossina) transmit deadly trypanosomes to human populations and domestic animals in sub-Saharan Africa. Some foci of Human African Trypanosomiasis due Trypanosoma brucei gambiense (g-HAT) persist southern Chad, where a program tsetse control was implemented against the local vector Glossina fuscipes 2018 Maro. We analyzed population genetics G. f. from Maro focus before (T0), one year (T1), 18 months (T2) after beginning efforts. Most captured displayed genetic profile...

Journal Article Effects of Pesticides Used on Citrus Grown in Spain the Mortality Ceratitis capitata (Diptera: Tephritidae) Vienna-8 Strain Sterile Males Get access María Juan–Blasco, Juan–Blasco 1 Instituto Valenciano de Investigaciones Agrarias (IVIA), Centro Protección Vegetal y Biotecnología, Unidad Asociada Entomología UJI-IVIA-CIB CSIC, E-46113-Moncada, Spain. Search for other works by this author on: Oxford Academic PubMed Google Scholar Beatriz Sabater–Muñoz, Sabater–Muñoz Rafael...

Abstract BACKGROUND: The sterile insect technique (SIT) is acknowledged around the world as an effective method for biological pest control of Ceratitis capitata (Wiedemann). Sterile insects are produced in biofactories where one key issue selection progenitors that have to transmit specific genetic characteristics. Recombinant individuals must be removed this colony renewed. Nowadays, task performed manually, a process extremely slow, painstaking and labour intensive, which sex identified....

Successful implementation of the sterile insect technique (SIT) against Aedes albopictus and Anopheles arabiensis relies on a continuous supply males. To meet this requirement, optimization mass-rearing techniques is needed. This study, therefore, aims to assess new cage (MRC) in terms egg production efficiency hatch rate (quality). In addition, adult survival was evaluated based adult-index for Ae. albopictus. Moreover, cage's suitability use mass An. compared that FAO/IAEA reference cage....