A5083449358- Fungal Infections and Studies
- Antifungal resistance and susceptibility
- Bacterial biofilms and quorum sensing
- Extracellular vesicles in disease
- Legionella and Acanthamoeba research
- Galectins and Cancer Biology
- Studies on Chitinases and Chitosanases
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Nanocomposite Films for Food Packaging
- Antimicrobial Peptides and Activities
- Nanoparticles: synthesis and applications
- Nail Diseases and Treatments
- Phytoplasmas and Hemiptera pathogens
- Bacterial Infections and Vaccines
- Plant Pathogens and Fungal Diseases
- Toxoplasma gondii Research Studies
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Virus-based gene therapy research
- Plant-Microbe Interactions and Immunity
- Parasitic Diseases Research and Treatment
- Heme Oxygenase-1 and Carbon Monoxide
- Dielectric properties of ceramics
- Integrated Circuits and Semiconductor Failure Analysis
- Industrial Vision Systems and Defect Detection
Universidade Federal do Rio de Janeiro
2019-2024
Instituto Federal de Educação Ciência e Tecnologia da Paraíba
2024
Universidade de Pernambuco
2023
Regular protocols for the isolation of fungal extracellular vesicles (EVs) are time-consuming, hard to reproduce, and produce low yields. In an attempt improve used EV isolation, we explored a model vesicle production after growth Cryptococcus gattii neoformans on solid media. Nanoparticle tracking analysis in combination with transmission electron microscopy revealed that C. produced EVs The properties cryptococcal varied according culture medium EV-producing species. detection was...
The ability to undergo morphological changes during adaptation distinct environments is exploited by Candida albicans and has a direct impact on biofilm formation virulence. Morphogenesis controlled diversity of stimuli, including osmotic stress, pH, starvation, presence serum, microbial components, among others.
Extracellular vesicles (EVs) are lipid bilayered compartments released by virtually all living cells, including fungi. Among the diverse molecules carried fungal EVs, a number of immunogens, virulence factors and regulators have been characterised. Within these components could potentially impact disease outcomes interacting with host. From this perspective, we previously demonstrated that EVs from Candida albicans be taken up activate macrophages dendritic cells to produce cytokines express...
ABSTRACT Systemic candidiasis remains a significant public health concern worldwide, with high mortality rates despite available antifungal drugs. Drug-resistant strains add to the urgency for alternative therapies. In this context, vaccination has reemerged as prominent immune-based strategy. Extracellular vesicles (EVs), nanosized lipid bilayer particles, carry diverse array of native fungal antigens, including proteins, nucleic acids, lipids, and glycans. Previous studies from our...
The small molecule (molecular mass <900 Daltons) composition of extracellular vesicles (EVs) produced by the pathogenic fungus Cryptococcus gattii is unknown, which limits understanding functions cryptococcal EVs. In this study, we analyzed molecules in samples obtained from solid cultures C. a combination chromatographic and spectrometric approaches, untargeted metabolomics. This analysis revealed previously unknown components EVs, including peptides with known biological other models....
Silver compounds are widely known for their antimicrobial activity, but can exert toxic effects to the host. Among strategies reduce its toxicity, incorporation into biopolymers has shown promising results. We investigated green syntheses of silver nanoparticles (AgNPs) and functionalization in a chitosan matrix (AgNPs@Chi) as potential treatment against Candida spp. Inhibitory concentrations ranging between 0.06 1 μg/ml were observed distinct species. Nanocomposite-treated cells displayed...
ABSTRACT Small molecules are components of fungal extracellular vesicles (EVs), but their biological roles only superficially known. NOP16 is a eukaryotic gene that required for the activity benzimidazoles against Cryptococcus deuterogattii . In this study, during phenotypic characterization C. mutants expected to lack expression, we observed reduced EV production. Whole-genome sequencing, RNA-Seq, and cellular proteomics revealed that, contrary our initial findings, these expressed Nop16...
Candida auris is a recently emerged global fungal pathogen, which causes life-threatening infections, often in healthcare settings. C. infections are worrisome because the fungus resistant to multiple antifungal drug classes. Furthermore, forms durable and difficult remove biofilms. Due relatively recent, resilient, nature of auris, we investigated whether it produces common virulence factor melanin. Melanin black-brown pigment typically produced following enzymatic oxidation aromatic...
The urgency surrounding
Sporotrichosis is an emerging mycosis caused by members of the genus Sporothrix . The disease affects humans and animals, particularly cats, which play important role in zoonotic transmission.
Acanthamoeba castellanii ( Ac ) is a species of free-living amoebae (FLAs) that has been widely applied as model for the study host-parasite interactions and characterization environmental symbionts. The sharing niches between potential pathogens, such fungi, favors associations these organisms. Through predatory behavior, enhances fungal survival, dissemination, virulence in their intracellular milieu, training pathogens granting subsequent success events infections to more evolved hosts....
The use of silver nanoparticles (AgNPs) embedded in polymeric matrix has acquired special attention as a strategy to reduce metal toxicity without losing its antimicrobial effect. In this work, the green synthesis AgNPs and their functionalization with chitosan (AgNPs@Chi) was performed, antifungal activity investigated against foremost species responsible for causing sporotrichosis, Sporothrix brasiliensis schenckii . vitro studies revealed inhibitory concentrations ranging from 0.12–1...
Small molecules are components of fungal extracellular vesicles (EVs), but their biological roles only superficially known.
ABSTRACT In this study, we conducted an in-depth analysis to characterize potential Acanthamoeba castellanii ( Ac ) proteins capable of recognizing fungal β-1,3-glucans. specifically anchors curdlan or laminarin, indicating the presence surface β-1,3-glucan-binding molecules. Using optical tweezers, strong adhesion laminarin- curdlan-coated beads was observed, highlighting their adhesive properties compared controls (characteristic time τ 46.9 and 43.9 s, respectively). Furthermore,...
ABSTRACT We conducted a comprehensive comparative analysis of extracellular vesicles (EVs) from two Acanthamoeba castellanii strains, Neff (environmental) and T4 (clinical). Morphological via transmission electron microscopy revealed slightly larger EVs (average = 194.5 nm) compared to more polydisperse 168.4 nm). Nanoparticle tracking (NTA) dynamic light scattering validated these differences. Proteomic the identified 1,352 proteins, with 1,107 common, 161 exclusive in Neff, 84 exclusively...
Aspergillosis cases by Aspergillus fumigatus have increased, along with fungal resistance to antifungals, urging the development of new therapies. Passive immunization targeting common antigens, such as chitin and β-glucans, are promising would eliminate need species-level diagnosis, thereby expediting therapeutic intervention. However, these polysaccharides poorly immunogenic. To overcome this drawback, we developed lectin-Fc(IgG) fusion proteins, Dectin1-Fc(IgG2a), Dectin1-Fc(IgG2b) wheat...
Abstract Fungal infections have increased in the last years, particularly associated to an increment number of immunocompromised individuals and emergence known or new resistant species, despite difficulties often time-consuming diagnosis. The controversial efficacy currently available strategies for their clinical management, apart from high toxicity severe side effects, has renewed interest research development broad antifungal alternatives. These encompass vaccines passive immunization...
Abstract The ability to undergo morphological changes during adaptation distinct environments is exploited by Candida albicans and has a direct impact on virulence. In this study, we investigated the influence of fungal extracellular vesicles (EVs) yeast growth, biofilm formation, morphogenesis in C. . Addition EVs ( Ca EVs) culture medium positively affected growth. Using crystal violet staining scanning electron microscopy (SEM), demonstrated that inhibited formation vitro By time-lapse...
Cryptococcus neoformans is a fungal pathogen that causes life-threatening infections in immunocompromised individuals, who often have some inflammatory condition and, therefore, end up using glucocorticoids, such as dexamethasone and methylprednisolone. Although the effects of this class molecules during cryptococcosis been investigated, their consequences for biology C. less explored. Here, we studied methylprednisolone on metabolism induction virulence factors neoformans. Our results...
The cell wall is a ubiquitous structure in the fungal kingdom, with some features varying depending on species. Additional external structures can be present, such as capsule of Cryptococcus neoformans ( Cn ), its major virulence factor, mainly composed glucuronoxylomannan (GXM), anti-phagocytic and anti-inflammatory properties. literature shows that other cryptococcal species even more evolutionarily distant species, Trichosporon asahii, T. mucoides , Paracoccidioides brasiliensis produce...
Emergomyces africanus is a highly fatal fungal pathogen affecting individuals with advanced HIV disease. Molecular patterns and ultrastructural aspects of E. are unknown, pathogenic models have not been investigated in detail. Since the cell wall fungi determinant for interaction host antifungal development, we characterized general properties components under different conditions growth vitro vivo. We also tested potential Galleria mellonella model infection. Transmission electron...
Fusarium oxysporum é um fungo conhecido por sua capacidade fitopatogênica e crescente ameaça frente à indivíduos imunossuprimidos devido seus amplos fatores de virulência. As vesículas extracelulares (VEs) são responsáveis carrear comunicação virulência, portanto o presente trabalho teve objetivo avaliar a metodologia centrifugação diferencial para isolamento VEs F. oxysporum. Foi realizado inóculo 1,2 x107 conídios/mL incubado durante 7 dias. A cada 24 horas foi retirado uma alíquota...
Volume 90, no. 8, iai.00232–22, 2022, https://doi.org/10.1128/iai.00232-22. In our original article, we discussed the associations between virulence mechanisms and extracellular vesicle (EV) formation in fungal pathogen Cryptococcus deuterogattii. We also posited a possible connection EV NOP16 gene based on observation of reduced production two independent mutants supposedly lacking expression this gene.