A5018585036- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- T-cell and B-cell Immunology
- Single-cell and spatial transcriptomics
- Immune responses and vaccinations
- Malaria Research and Control
- Hematopoietic Stem Cell Transplantation
- HIV Research and Treatment
- Cell Adhesion Molecules Research
- Acute Myeloid Leukemia Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Mosquito-borne diseases and control
- Erythropoietin and Anemia Treatment
- Hematological disorders and diagnostics
- Gene Regulatory Network Analysis
- Acute Lymphoblastic Leukemia research
- Cancer Cells and Metastasis
European Molecular Biology Laboratory
2023-2024
Heidelberg University
2021-2024
European Molecular Biology Organization
2021-2024
University Hospital Heidelberg
2022-2024
European Molecular Biology Laboratory
2020-2024
Abstract The redirection of T cells has emerged as an attractive therapeutic principle in B cell non-Hodgkin lymphoma (B-NHL). However, a detailed characterization lymphoma-infiltrating across B-NHL entities is missing. Here we present in-depth reference map nodal B-NHL, based on cellular indexing transcriptomes and epitopes, receptor sequencing, flow cytometry multiplexed immunofluorescence applied to 101 lymph nodes from patients with diffuse large cell, mantle follicular or marginal zone...
Acute myeloid leukemia (AML) is a hematologic malignancy for which allogeneic hematopoietic cell transplantation (allo-HCT) often remains the only curative therapeutic approach. However, incapability of T cells to recognize and eliminate residual stem might lead an insufficient graft-versus-leukemia (GVL) effect relapse. Here, we performed single-cell RNA-sequencing (scRNA-seq) on bone marrow (BM) lymphocytes CD34+ 6 patients with AML 100 days after allo-HCT identify T-cell signatures...
In malaria-naïve children and adults, Plasmodium falciparum -infected red blood cells ( Pf -iRBCs) trigger fever other symptoms of systemic inflammation. However, in endemic areas where individuals experience repeated infections over many years, the risk -iRBC-triggered inflammatory decreases with cumulative exposure. The molecular mechanisms underlying these clinical observations remain unclear. Age-stratified analyses uninfected, asymptomatic Malian before malaria season revealed that...
Residing at the top of hematopoietic hierarchy, long-term stem cells (HSCs) are capable self-renewal and sustained blood cell regeneration. Over past decades, single-cell clonal analyses have revealed substantial functional molecular heterogeneity within this compartment, challenging notion that is inherently tied to balanced, multi-lineage production. However, a cohesive model explains relationships among these diverse HSC states remains elusive. Here, we combined transplantations over...
Abstract Intratumor heterogeneity underpins cancer pathogenesis and evolution, although it is typically considered independent from the differentiation processes that drive physiological cell-type diversity. As types subtypes arise different cell types, we investigated whether cellular influences intratumor heterogeneity. Nodal B-cell non-Hodgkin lymphomas are a diverse set of cancers originating stages maturation. Through single-cell transcriptome surface epitope profiling (CITE-Seq)...
Abstract Bone marrow mesenchymal stromal cells (BMSCs) can differentiate into adipocytes and osteoblasts, are important regulators of the haematopoietic system. Ageing associates with an increased ratio bone to osteoblasts immune dysregulation. Here, we carried out integrative multiomics analysis ATAC-Seq, RNA-Seq proteomics data from primary human BMSCs in a healthy cohort age between 20 - 60. We identified age-sensitive elements uniquely affecting each molecular level where transcription...
Abstract T cell acute lymphoblastic leukemia (T-ALL) is an aggressive predominantly affecting adolescents and young adults. T-ALL relapses are characterized by chemotherapy resistance, cellular heterogeneity dismal outcome. To gain a deeper understanding of the mechanisms driving relapse, we conducted single-cell full-length RNA sequencing 13 matched pediatric patient-derived xenografts (PDX) samples obtained at initial diagnosis generating to date most comprehensive longitudinal single...
In malaria-naïve children and adults, Plasmodium falciparum -infected red blood cells ( Pf -iRBCs) trigger fever other symptoms of systemic inflammation. However, in endemic areas where individuals experience repeated infections over many years, the risk -iRBC-triggered inflammatory decreases with cumulative exposure. The molecular mechanisms underlying these clinical observations remain unclear. Age-stratified analyses monocytes collected from uninfected, asymptomatic Malian before malaria...
Topic: 20. Lymphoma Biology & Translational Research Background: Intratumor heterogeneity (ITH) is increasingly recognized as an essential factor in the variability of B cell non-Hodgkin lymphoma (B-NHL) patient outcomes. It well established that distinct nodal (NBNHL) entities originate from different stages humoral affinity maturation process, but drivers ITH are not understood. Aims: In this study, we aimed to characterize B-NHL and investigate its relationship with process. Methods: 51...
Topic: 20. Lymphoma Biology & Translational Research Background: The function of lymph nodes depends on the coordinated structure defined by node stromal cells (LNSC), which consist endothelial as well chemokine-secreting mesenchymal cells. In B cell non-Hodgkin lymphoma (B-NHL), LN is disrupted, indicating that LNSCs play a crucial role within tumor microenvironment (TME). Nevertheless, molecular mechanisms reprogramming in B-NHL are not comprehended. Aims: Here, we studied TME order to...