A5101520952- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Bacterial Genetics and Biotechnology
- Bacillus and Francisella bacterial research
- Infectious Diseases and Tuberculosis
- Trace Elements in Health
- Diagnosis and treatment of tuberculosis
- RNA modifications and cancer
- Cancer therapeutics and mechanisms
- Biochemical and Molecular Research
- Cancer-related gene regulation
- Epigenetics and DNA Methylation
- Enzyme Structure and Function
- Drug Transport and Resistance Mechanisms
- Peptidase Inhibition and Analysis
- Plant Micronutrient Interactions and Effects
- Genetics and Neurodevelopmental Disorders
- Bacterial biofilms and quorum sensing
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- Yersinia bacterium, plague, ectoparasites research
- Hepatitis B Virus Studies
- HIV Research and Treatment
- Iron Metabolism and Disorders
- Pharmacological Effects and Toxicity Studies
Institute of Genomics and Integrative Biology
2013-2020
University of Washington
2018-2020
Council of Scientific and Industrial Research
2011-2019
University of Delhi
2015
All India Institute of Medical Sciences
2005
Department of Biotechnology
2005
Children's Hospital of Richmond at VCU
1991
ABSTRACT Mycobacterium tuberculosis and M. bovis infect animals humans. Their epidemiologies in developed developing countries differ, owing to differences the implementation of preventive measures (World Health Organization, 1999). Identification differentiation these closely related mycobacterial species would help determine source, reservoirs infection, disease burden due diverse pathogens. The utility hupB gene (Rv2986c , or Mb3010c ) differentiate was evaluated by a PCR-restriction...
During protein synthesis, translation elongation factor Tu (Ef-Tu) is responsible for the selection and binding of cognate aminoacyl-tRNA to acceptor site on ribosome. The activity Ef-Tu dependent its interaction with GTP. Posttranslational modifications, such as phosphorylation, are known regulate in several prokaryotes. Although a study Mycobacterium tuberculosis phosphoproteome showed be phosphorylated, role phosphorylation regulation has not been studied. In this report, we show that M....
Abstract Extrapulmonary manifestations constitute 15 to 20% of tuberculosis cases, with lymph node (LNTB) as the most common form infection. However, diagnosis and treatment advances are hindered by lack understanding LNTB biology. To identify host response, Mycobacterium infected nodes from patients were studied means transcriptomics quantitative proteomics analyses. The selected targets obtained comparative analyses validated PCR immunohistochemistry. This approach provided expression data...
Mycobacterium tuberculosis modulates expression of various metabolism-related genes to adapt in the adverse host environment. The gene coding for M. S-adenosylhomocysteine hydrolase (Mtb-SahH) is essential optimal growth and protein product involved intermediary metabolism. However, relevance SahH mycobacterial physiology unknown. In this study, we analyze role Mtb-SahH regulating homocysteine concentration surrogate smegmatis. catalyzes reversible hydrolysis adenosine demonstrate that...
PrkC is a conserved Ser/Thr protein kinase encoded in Bacillus anthracis genome. shown to be important for B. pathogenesis, but little known about its other functions and phosphorylated substrates. Systemic analyses indicate the compelling role of phosphorylating multiple substrates, including essential chaperone GroEL. Through mass spectrometry, we identified that phosphorylates GroEL on six threonine residues are distributed three canonical regions. Phosphorylation facilitates...
ABSTRACT Nitric oxide (NO·) produced by mammalian cells exerts antimicrobial actions that result primarily from the modification of protein thiols ( S -nitrosylation) and metal centers. A comprehensive approach was used to identify novel targets NO· in Salmonella enterica serovar Typhimurium S. Typhimurium). Newly identified include zinc metalloproteins required for DNA replication repair (DnaG, PriA, TopA), synthesis (AlaS RpmE), various metabolic activities (ClpX, GloB, MetE, PepA, QueC)....
ABSTRACT Endometrial biopsy samples derived from 393 patients with assorted gynecological complaints were investigated for mycobacterial infection. By employment of four different techniques, pathogens detected irrespective the nature/type clinical complaint. Mycobacterium tuberculosis was predominant pathogen among investigated.
ABSTRACT The paucibacillary nature of the cerebrospinal fluid (CSF) has been a major obstacle in diagnosis human tuberculous meningitis (TBM). This study shows that with molecular techniques direct precise determination to species level mycobacterial pathogens can be made. present report describes utility nested PCR (N-PCR) assay (A. Mishra, A. Singhal, D. S. Chauhan, V. M. Katoch, K. Srivastava, Thakral, Bharadwaj, Sreenivas, and H. Prasad, J. Clin. Microbiol. 43:5670-5678, 2005) detecting...
Background Mycobacterium ulcerans, the causative agent of Buruli ulcer in humans, is unique among members genus due to presence virulence determinant megaplasmid pMUM001. This plasmid encodes multiple virulence-associated genes, including mup011, which an uncharacterized Ser/Thr protein kinase (STPK) PknQ. Methodology/Principal Findings In this study, we have characterized PknQ and explored its interaction with MupFHA (Mup018c), a FHA domain containing also encoded by was found interact...
Bacterial alternative sigma factors are mostly regulated by a partner-switching mechanism. Regulation of the virulence-associated factor SigF Mycobacterium tuberculosis has been an area intrigue, with having more predicted regulators than other in this organism. Rv1364c is one such regulator, mechanism which confounded presence both anti-sigma and antagonist functions single polypeptide. Using protein binding phosphorylation assays, we demonstrate that domain mediates autophosphorylation its...
Post-translational modifications such as phosphorylation, nitrosylation, and pupylation modulate multiple cellular processes in Mycobacterium tuberculosis. While protein methylation at lysine arginine residues is widespread eukaryotes, to date only two methylated proteins Mtb have been identified. Here, we report the identification of and/or nine mycobacterial proteins. Among identified, chose MtrA, an essential response regulator a two-component signaling system, which gets on examine...
Genetic studies pertaining to effector molecules have been pivotal in schizophrenia research.
Salmonella enterica serovar Typhimurium is an intracellular pathogen that elicits nitric oxide (NO·) production by host macrophages. NO· a potent antimicrobial mediator with diverse targets, including protein thiols and metal centers. The mobilization of zinc from metalloproteins increases the availability free zinc, which detrimental to bacterial cells, but precise mechanism cytotoxicity uncertain. Here, we show excess results in mismetallation essential iron-containing enzyme peptide...