A5050261566- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Sphingolipid Metabolism and Signaling
- Immunodeficiency and Autoimmune Disorders
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Autophagy in Disease and Therapy
- Lipid metabolism and biosynthesis
- Bacteriophages and microbial interactions
- Mass Spectrometry Techniques and Applications
- Immune Response and Inflammation
- interferon and immune responses
- Immune cells in cancer
- Genomics and Phylogenetic Studies
- Biotin and Related Studies
- Lipid Membrane Structure and Behavior
- Click Chemistry and Applications
- Metabolomics and Mass Spectrometry Studies
- Cancer therapeutics and mechanisms
Stony Brook University
2021-2023
Institute of Genomics and Integrative Biology
2013-2019
Academy of Scientific and Innovative Research
2017-2019
Pulmonary tuberculosis (TB) exhibits granulomatous inflammation, a site of controlling bacterial dissemination at the cost host tissue damage. Intrigued by granuloma type-dependent expression inflammatory markers in TB, we sought to investigate underlying metabolic changes that drive amplification inflammation TB. Here, show an association higher necrotic granulomas with presence triglyceride (TG)-rich foamy macrophages. The conspicuous absence these macrophages solid identified link between...
Growing evidence suggests the importance of lipid metabolism in pathogenesis tuberculosis. Neutral lipids form majority a caseous granuloma, pathology characteristic Cytosolic droplets (LDs) macrophages store house these and have been demonstrated to contribute inflammatory response infection. The proteome reflects mechanisms active under condition. However, infection induced changes dynamic organelles remains elusive. Here, we employed quantitative proteomics identify alterations upon with...
Background The failure of Mycobacterium bovis Bacille Calmette-Guérin to impart satisfactory protection against adult pulmonary tuberculosis has necessitated the development more effective TB vaccines. assumption that vaccine strain should be antigenically as similar possible disease causing pathogen led evaluation M.tuberculosis mutants candidate Methods/Principal Findings In this study, we have generated a mutant (Mtb∆mms) by disrupting 3 virulence genes encoding mycobacterial secretory...
The mycobacterial cell wall is a chemically complex array of molecular entities that dictate the pathogenesis Mycobacterium tuberculosis. Biosynthesis and maintenance this dynamic entity in physiology still poorly understood. Here we demonstrate requirement for M. tuberculosis MmpL11 architecture stability response to surface stress. In presence detergent like Tyloxapol, mmpL11 deletion mutant suffered from severe growth attenuation as result altered membrane polarity, permeability...
ABSTRACT Mycobacterium tuberculosis , a successful human pathogen, utilizes multiple carbon sources from the host but adapts to fatty-acid-rich environment in vivo . We sought delineate physiologic response of M. lipid-rich by using differentiated adipocytes as model system. Global transcriptome profiling based on RNA sequencing was performed for bacilli infected and preadipocytes. Genes involved de novo fatty acid synthesis were downregulated, while those predicted be triglyceride...
Bacterial alternative sigma factors are mostly regulated by a partner-switching mechanism. Regulation of the virulence-associated factor SigF Mycobacterium tuberculosis has been an area intrigue, with having more predicted regulators than other in this organism. Rv1364c is one such regulator, mechanism which confounded presence both anti-sigma and antagonist functions single polypeptide. Using protein binding phosphorylation assays, we demonstrate that domain mediates autophosphorylation its...
A previous laboratory study involving wild type, mutant and devR/dosR complemented strains of Mycobacterium tuberculosis reported the attenuation phenotype strain, Comp1. This was intriguing since parental strain H37Rv, devR (Mut1) additional strains, Comp9 Comp11, were virulent in guinea pig model.Towards deciphering mechanism underlying Comp1, a whole genome sequencing approach undertaken. Eight Single Nucleotide Polymorphisms (SNPs) unique to Comp1 identified. Of these, 5 SNPs...
SUMMARY The cell wall of mycobacteria plays a key role in interactions with the environment and its ability to act as selective filter is crucial bacterial survival. Proteins enable this function by mediating import export diverse metabolites from ions lipids proteins. Accurately identifying proteins an important step assigning function, especially many mycobacterial lack functionally characterized homologues. Current methods for protein localization have inherent limitations that reduce...
Abstract Tuberculosis granulomas represent a site of controlling bacterial dissemination at the cost host tissue damage. Within heterogenous array TB granulomas, some contain triglyceride (TG) rich foamy macrophages, etiology and function which remains largely unexplained. Here we show that necrosis tuberculosis lesions M. (Mtb) infected macrophages elicits bystander response storage. We elucidate role for RD1 region mycobacterial genome to be key player in this phenomenon. TG storage...