A5046586712- Cancer, Stress, Anesthesia, and Immune Response
- Brain Metastases and Treatment
- Alzheimer's disease research and treatments
- Ubiquitin and proteasome pathways
- Nanoplatforms for cancer theranostics
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Extracellular vesicles in disease
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Nicotinic Acetylcholine Receptors Study
- Advanced Proteomics Techniques and Applications
- Neuroscience and Neuropharmacology Research
- interferon and immune responses
- Bacterial Genetics and Biotechnology
- RNA and protein synthesis mechanisms
- Advanced Breast Cancer Therapies
- Computational Drug Discovery Methods
- Tuberculosis Research and Epidemiology
- Cancer-related gene regulation
- Curcumin's Biomedical Applications
- Mass Spectrometry Techniques and Applications
New York University
2016-2025
NYU Langone Health
2016-2025
NYU Langone’s Laura and Isaac Perlmutter Cancer Center
2022-2024
New York Proton Center
2023
New York University Florence
2023
Center for Neuro-Oncology
2022
Indiana University School of Medicine
2022
Rockefeller University
2006-2021
Institute for Systems Biology
2021
York University
2018
Passive transfer of broadly neutralizing HIV antibodies can prevent infection, which suggests that vaccines elicit such would be protective. Thus far, however, few occur naturally have been characterized. To determine whether these are part a larger group related molecules, we cloned 576 new from four unrelated individuals. All individuals produced expanded clones potent CD4-binding-site mimic binding to CD4. Despite extensive hypermutation, the shared consensus sequence 68 immunoglobulin H...
A method for rapid sequencing of intact proteins simultaneously from the N and C termini (1–2 s) with online chromatography is described applied to characterization histone H3.1 posttranslational modifications identification an additional member H2A gene family. Proteins are converted gas-phase multiply charged positive ions by electrospray ionization then allowed react fluoranthene radical anions. Electron transfer protein promotes random dissociation N—Cα bonds backbone. Multiply fragment...
Abstract Radiotherapy (RT) used at immunogenic doses leads to accumulation of cytosolic double-stranded DNA (dsDNA) in cancer cells, which activates type I IFN (IFN-I) via the cGAS/STING pathway. Cancer cell–derived IFN-I is required recruit BATF3-dependent dendritic cells (DC) poorly tumors and trigger antitumor T-cell responses combination with immune checkpoint blockade. We have previously demonstrated that exonuclease TREX1 regulates radiation immunogenicity by degrading dsDNA....
Accumulation of phosphorylated tau is a key pathological feature Alzheimer's disease. Phosphorylated accumulation causes synaptic impairment, neuronal dysfunction and formation neurofibrillary tangles. The actions are mediated by surrounding proteins; however, comprehensive understanding the proteins that interacts with in disease surprisingly limited. Therefore, aim this study was to determine interactome. To end, we used two complementary proteomics approaches: (i) quantitative performed...
We describe design, rapid assembly, and characterization of synthetic yeast Sc2.0 chromosome VI (synVI). A mitochondrial defect in the synVI strain mapped to synonymous coding changes within PRE4 (YFR050C), encoding an essential proteasome subunit; reduced Pre4 protein accumulation by half. Completing specifies consolidation 16 chromosomes into a single strain. investigated phenotypic, transcriptional, proteomewide consequences poly-synthetic strains. Another "bug" was discovered through...
Significance Mycobacterium tuberculosis (Mtb) uses type VII secretion systems to secrete cognate protein pairs that alter host interactions. Here, we address the contributions of ESX-3 system Mtb growth and pathogenesis through a combination genetics, proteomics, studies both in vitro vivo. is demonstrated play critical role iron acquisition pair pf proteins belonging PE–PPE family (PE5–PPE4). In vivo, importance PE5–PPE4 was found depend upon genotype, likely reflecting capacity restrict...
Amyloid plaques contain many proteins in addition to beta amyloid (Aβ). Previous studies examining plaque-associated have shown these additional are important; they provide insight into the factors that drive plaque development and potential biomarkers or therapeutic targets for Alzheimer's disease (AD). The aim of this study was comprehensively identify enriched using unbiased proteomics two subtypes early onset AD: sporadic AD (EOAD) Down Syndrome (DS) with AD. We focused our on as drivers...
Abstract Proteomic studies of human Alzheimer’s disease brain tissue have potential to identify protein changes that drive disease, and new drug targets. Here, we analyse 38 published proteomic studies, generating a map in across thirteen regions, three stages (preclinical mild cognitive impairment, advanced disease), proteins enriched amyloid plaques, neurofibrillary tangles, cerebral angiopathy. Our dataset is compiled into searchable database (NeuroPro). We found 848 were consistently...
Abstract The prevalence of epilepsy is increased among Alzheimer’s Disease (AD) patients and cognitive impairment common people with epilepsy. Epilepsy AD are linked but the shared pathophysiological changes remain poorly defined. We aim to identify protein differences associated using published proteomics datasets. observed a highly significant overlap in AD: 89% (689/777) proteins altered hippocampus were significantly advanced AD. Of both AD, 340 same direction, while 216 opposite...
Down syndrome (DS) is strongly associated with Alzheimer's disease (AD) due to APP overexpression, exhibiting Amyloid-β (Aβ) and Tau pathology similar early-onset (EOAD) late-onset AD (LOAD). We evaluated the Aβ plaque proteome of DS, EOAD, LOAD using unbiased localized proteomics on post-mortem paraffin-embedded tissues from four cohorts (n = 20/group): DS (59.8 ± 4.99 y/o), EOAD (63 4.07 (82.1 6.37 controls (66.4 13.04). identified differentially abundant proteins when comparing plaques...
We describe the design and performance of a prototype high hybrid mass spectrometer. This instrument consists linear quadrupole ion trap (QLT) coupled to Fourier transform cyclotron resonance analyzer (FTMS). configuration provides rapid automated MS MS/MS analyses, similar "data dependent scanning" found on standard 3-D Paul traps, but with substantially improved internal scan dynamic range, measurement accuracy, resolution, detection limits. Sequence analysis peptides at zeptomole level is...
Antibodies can protect from Plasmodium falciparum (Pf) infection and clinical malaria disease. However, in the absence of constant reexposure, serum immunoglobulin (Ig) levels rapidly decline full protection symptoms is lost, suggesting that B cell memory functionally impaired. We show at single level natural Pf induces development classical cells (CM) atypical (AtM) produce broadly neutralizing antibodies against blood stage parasites. CM AtM contribute to anti-Pf IgG production, but only...