A5031840950- Clostridium difficile and Clostridium perfringens research
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Peptidase Inhibition and Analysis
- Drug Transport and Resistance Mechanisms
- Viral gastroenteritis research and epidemiology
- Microscopic Colitis
- RNA modifications and cancer
- Gut microbiota and health
- Genomics and Phylogenetic Studies
- Probiotics and Fermented Foods
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Bacterial Identification and Susceptibility Testing
- Bacteriophages and microbial interactions
- Helicobacter pylori-related gastroenterology studies
- Cholesterol and Lipid Metabolism
- Cancer Mechanisms and Therapy
- Cell Adhesion Molecules Research
- FOXO transcription factor regulation
- Trace Elements in Health
- PARP inhibition in cancer therapy
- Sirtuins and Resveratrol in Medicine
- Microbial Metabolism and Applications
- MicroRNA in disease regulation
- Biosensors and Analytical Detection
Mahidol University
2016-2025
South Dakota State University
2018
New York State College of Veterinary Medicine
2009-2012
Cornell University
2009-2012
University of Cambridge
2003-2007
A complex microbial community in the gut may prevent colonization of enteric pathogens such as
The human breast cancer resistance protein (BCRP, also know as ABCG2, MXR, or ABCP) is one of the more recently discovered ATP-binding cassette (ABC) transporters that confer on cells by mediating multidrug efflux. In present study, we have obtained functional expression BCRP in Gram-positive bacterium Lactococcus lactis. conferred lactococcal cells, which was based ATP-dependent drug extrusion. BCRP-mediated ATPase and transport activities were inhibited BCRP-specific modulator...
LmrA is an ATP binding cassette (ABC) multidrug transporter in Lactococcus lactis that a structural and functional homologue of the human resistance P-glycoprotein MDR1 (ABCB1). also homologous to MsbA, essential ABC Escherichia coli involved trafficking lipids, including Lipid A. We have compared substrate specificities MsbA detail. Surprisingly, was able functionally substitute for temperature-sensitive mutant E. WD2 at non-permissive temperatures, suggesting could transport exhibited...
Nasopharyngeal carcinoma (NPC) is an uncommon cancer, which has a distinctive ethnic and geographic distribution. Etiology of NPC considered to be related with complex interaction environmental genetic factors as well Epstein-Barr virus infection. Since located in the silent painless area, disease usually therefore diagnosed at advanced stages; hence early detection difficult. Furthermore, understanding molecular pathogenesis still lacking, pondering identification effective prognostic...
ABSTRACT Clostridium difficile is a gram-positive, spore-forming enteric anaerobe which can infect humans and wide variety of animal species. Recently, the incidence severity human C. infection has markedly increased. In this study, we evaluated genomic content 73 strains isolated from humans, horses, cattle, pigs by comparative hybridization with microarrays containing coding sequences 630 QCD-32g58. The sequenced genome strain was used as reference to define candidate core explore...
Microarray-based comparative genome hybridisations (CGH) and sequencing of Clostridium difficile isolates have shown that the genomes this species are highly variable. To further characterize their variation, we employed integration data from CGH, putative cellular pathways. Transcontinental strain comparison using CGH confirmed emergence a human-specific hypervirulent cluster. However, there was no correlation between total toxin production phenotype, indicating possibility involvement...
Clostridioides difficile infection (CDI) is a major public health issue, driven by antibiotic resistance and frequent recurrence. CD2068, an ABC protein in C. difficile, associated with drug resistance, making it potential target for novel therapies. This study explored FDA-approved non-antibiotic drugs their ability to inhibit CD2068 through screening experimental validation. Thioridazine exhibited moderate binding affinity inhibited its ATP hydrolysis activity. It also suppressed the...
The human BCRP (breast cancer resistance protein, also known as ABCG2) is an ABC (ATP-binding cassette) transporter that extrudes various anticancer drugs from cells, causing multidrug resistance. To study the molecular determinants of drug specificity in more detail, we have expressed wild-type (BCRP-R) and drug-selected cell line-associated R482G (Arg482→Gly) mutant (BCRP-G) Lactococcus lactis. Drug rate efflux BCRP-expressing cells were proportional to expression level protein affected by...
Background and Aim . Cholangiocarcinoma (CCA) is the most frequent biliary malignancy, which poses high mortality rate due to lack of early detection. Hence, CCA cases are present at advanced late stages with local or distant metastasis time diagnosis. Currently available tumor markers including CA19-9 CEA inefficient limited usage low sensitivity specificity. Here, we attempt identify serum for that can effectively distinguish from benign tract diseases (BBTDs). Methods Serum samples 19...
Clostridium difficile is recognized as a problematic pathogen, causing severe enteric diseases including antibiotic-associated diarrhea and pseudomembranous colitis. The emergence of antibiotic resistant C. has driven search for alternative anti-infection modalities. A promising strategy controlling bacterial infection includes the use bacteriophages their gene products. Currently, knowledge phages active against still relatively limited by fact that isolation this organism technically...
Lactic acid bacteria, Enterococcus faecium and Lactococcus lactis, previously isolated from Thai fermented sausages were elucidated their probiotic properties especially in the control of Clostridium difficile 630. Both isolates survived simulated gastric solution at pH 3 followed intestinal 8. The presence skimmed milk also helped bacteria to survive through acidic alkaline gastrointestinal conditions. adhesion both tested using a human colon adenocarcinoma cell line. result showed that...
A gnotobiotic Gallus gallus (chicken) model was developed to study the dynamics of intestinal microflora from hatching 18 days age employing metagenomics. Intestinal samples were collected a local population feral chickens and administered orally germfree 3-day-old chicks. Animals euthanized on 9 postinoculation, subjected metagenomic analysis. On day 18, five most prevalent phyla Bacteroidetes (43.03 ± 3.19%), Firmicutes (38.51 2.67%), Actinobacteria (6.77 0.7%), Proteobacteria (6.38...
Nasopharyngeal carcinoma (NPC) is a prevalent cancer in Southeast Asia, but effective treatment options remain limited, and chemotherapy has high resistance rate. Asiatic acid (AA), triterpenoid found Centella asiatica, shown anticancer activity various cancers. Therefore, this study aims to investigate the effects mechanisms of AA NPC cell lines. The on cytotoxicity, apoptosis, migration were determined TW-01 SUNE5-8F Western blot analysis was performed evaluate protein expression levels...
Clostridium difficile is well recognized as the most common infectious cause of nosocomial diarrhea. The incidence and severity C. infection (CDI) increasing worldwide. Here, we evaluated simultaneously transcriptional changes in human colorectal epithelial Caco-2 cells after infection. A total 271 transcripts 207 were significantly differentially expressed at 1 time point during CDI. We used gene ontology annotations protein-protein network interactions to underline a framework target...
A pig ligated loop model was used to analyze the in vivo transcriptome response of Clostridium difficile. Bacterial RNA from loops retrieved at different times and for microarray analysis. Several virulence-associated genes involved sporulation cascade were differentially expressed (DE). In concordance with observed upregulation toxin microarray, enzyme-linked immunosorbent assay estimation total showed high amounts loops. that absent primary annotation C. difficile 630 but annotated a...
Thermophilic microorganisms and their enzymes have been utilized in various industrial applications. In this work, we isolated characterized thermophilic anaerobic bacteria with the cellulose hemicellulose degrading activities from a tropical dry deciduous forest northern Thailand. Out of 502 soil bacteria, 6 isolates, identified as Thermoanaerobacterium sp., displayed an ability to utilize wide range oligosaccharides lignocellulosic substrates. The isolates exhibited significant cellulase...
Endolysin is a phage-encoded cell-wall hydrolase which degrades the peptidoglycan layer of bacterial cell wall. The enzyme often expressed at late stage phage lytic cycle and required for progeny escape. Endolysins bacteriophage that infect Gram-positive bacteria comprises two domains: binding domain (CBD). Although catalytic endolysin relatively well-studied, precise role CBD ambiguous remains controversial. Here, we focus on function from recently isolated Clostridioides difficile phage....
Antibiotic resistance is a major concern in Clostridium difficile, the causative agent of antibiotic-associated diarrhea. Reduced susceptibility to first- and second-line agents widespread, therefore various attempts have been made seek alternative preventive therapeutic strategies against this pathogen. In work, antimicrobial properties asiatic acid were evaluated C. difficile. Asiatic displayed substantial inhibitory effects on 19 difficile isolates collected from different sources with...