A5024504306- Microbial Natural Products and Biosynthesis
- Genomics and Phylogenetic Studies
- Insect Resistance and Genetics
- Enzyme Production and Characterization
- Marine Sponges and Natural Products
- Studies on Chitinases and Chitosanases
- Fungal Biology and Applications
- Bacterial Genetics and Biotechnology
- Probiotics and Fermented Foods
- Entomopathogenic Microorganisms in Pest Control
- Microbial Community Ecology and Physiology
- Plant Pathogens and Fungal Diseases
- Protein Hydrolysis and Bioactive Peptides
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Biofuel production and bioconversion
- Plant-Microbe Interactions and Immunity
- Insect and Pesticide Research
- Plant Disease Resistance and Genetics
- Biochemical effects in animals
- Enzyme Catalysis and Immobilization
- Biosensors and Analytical Detection
- Legume Nitrogen Fixing Symbiosis
- Salmonella and Campylobacter epidemiology
- Phytochemical compounds biological activities
King Mongkut's University of Technology Thonburi
2022-2024
Mahidol University
2012-2021
Bangkok University
2020
Osaka University
1983-2019
National Center for Genetic Engineering and Biotechnology
2011-2013
Office of the Higher Education Commission
2011
Ministry of Education
2011
In-Q-Tel
2007
RT-LAMP to detect SARS-CoV-2: in a positive sample, leads color change from pink yellow.
Extracellular endoxylanase (1, 4-β-D-xylan xylanohydrolase [EC 3.2.1.8]) produced by Bacillus pumilus IPO, an isolate from soil in Thailand, was purified to homogeneity. The optimum pH and temperature of the xylanase for hydrolysis larchwood xylan were 6.5 40°C. Its molecular weight estimated be 24, 000 dalton SDS-polyacrylamide gel electrophoresis 20, equilibrium centrifugation. number amino acid residues per molecule calculated 185, including one cysteine. maximumdegree 25%; xylobiose,...
The complete nucleotide sequence of the xylanase (EC 3.2.1.8) gene ( xynA and its flanking regions Bacillus pumilus IPO, a hyperproducer xylanase, was determined. A 684 bp open reading frame for observed. amino acid N‐terminal region determined to be Arg‐Thr‐Ile‐Thr‐, suggesting processing at Ala 27 pre‐xylanase. composition M r (22384) deduced from DNA agreed with results obtained purified enzyme. signal consisted acids, which 3 were basic residues in near N‐terminus 15 hydrophobic...
Five new anti-trypanosomal macrolides, actinoallolides A-E (1-5), were discovered from the cultured broth of Actinoallomurus fulvus MK10-036. The structures actinoallolides, including absolute stereochemistry, elucidated by a combination spectroscopic analyses and series chemical derivatization studies. Actinoallolide A showed most potent selective in vitro activity without cytotoxicity. class promising lead compounds was identified for development drugs both sleeping sickness Chagas disease.
Phytophthora is an important, highly destructive pathogen of many plants, which causes considerable crop loss, especially durians in Thailand. In this study, we selectively isolated Streptomyces from the rhizosphere soil with a potent anti-oomycete activity against palmivora CbP03. Two strains (SNN087 and SNN289) demonstrated exceptional plant growth-promoting properties pot experiment. Both promoted mung bean (Vigna radiate) growth effectively both sterile non-sterile soils. Metagenomic...
Abstract Background Spathaspora passalidarum is a yeast with the highly effective capability of fermenting several monosaccharides in lignocellulosic hydrolysates, especially xylose. However, this was shown to be sensitive furfural released during pretreatment and hydrolysis processes lignocellulose biomass. We aimed improve tolerance previously isolated S. CMUWF1−2, which presented thermotolerance no detectable glucose repression, via adaptive laboratory evolution (ALE). Results An adapted...
Colletotrichum is one of the most widespread and important genus plant pathogenic fungi worldwide. Various species are causative agents anthracnose disease in plants, which a severe problem to agricultural crops particularly Thailand. These phytopathogens usually controlled using chemicals; however, use these can lead environmental pollution. Potential non-chemical control strategies for include bacteria capable producing anti-fungal compounds such as actinomycetes spp., that comprise large...
Several Gram-negative pathogenic bacteria employ N -acyl-L-homoserine lactone (HSL) quorum sensing (QS) system to control their virulence traits. Degradation of acyl-HSL signal molecules by quenching enzyme (QQE) results in a loss pathogenicity QS-dependent organisms. The QQE activity actinomycetes rhizospheric soil and inside plant tissue was explored order obtain novel strains with high HSL-degrading activity. Among 344 132 endophytic isolates, 127 (36.9%) 68 (51.5%) them, respectively,...
Lactic acid bacteria, Enterococcus faecium and Lactococcus lactis, previously isolated from Thai fermented sausages were elucidated their probiotic properties especially in the control of Clostridium difficile 630. Both isolates survived simulated gastric solution at pH 3 followed intestinal 8. The presence skimmed milk also helped bacteria to survive through acidic alkaline gastrointestinal conditions. adhesion both tested using a human colon adenocarcinoma cell line. result showed that...
Acellular Pertussis vaccines against whooping cough caused by Bordetella pertussis present a much-improved safety profile compared to the original vaccine of killed whole cells. The principal antigen acellular vaccine, Toxin (PT), must be chemically inactivated obtain corresponding toxoid (PTd). This process, however, results in extensive denaturation antigen. development containing PTd or recombinant PT (rPT) with S1, Filamentous Hemagglutinin (FHA), and Pertactin (PRN) has shown that yield...
Abstract BACKGROUND Mixed larvae and pupae of weaver ant ( Oecophylla smaragdina ) are widely used as an important food ingredient in regions Thailand. They have high nutritional values comprise 53% protein 13% lipid. Peptides derived from proteins been shown to possess biological activities. RESULTS pepsin trypsin digestion these were purified based on angiotensin‐converting enzyme ACE inhibitory antioxidant activities, their amino acid sequences identified by liquid chromatography–tandem...
Glucose dehydrogenases (GluDH) from Bacillus species offer several advantages over other NAD(P)H regeneration systems including high stability, inexpensive substrate, thermodynamically favorable reaction and flexibility to regenerate both NADH NADPH. In this research, characteristics of GluDH amyloliquefaciens SB5 (GluDH-BA) was reported for the first time. Despite a highly similar amino acid sequence when comparing with subtilis (GluDH-BS), GluDH-BA exhibited significantly higher specific...
We characterized a new quorum-sensing regulator, PlcRa, which is present in various members of the B. cereus group and identified signaling heptapeptide for PlcRa activity: PapRa7. demonstrated that 3D structural paralog PlcR using sequence analysis homology modeling. A comparison transcriptomes at onset stationary phase ΔplcRa mutant wild-type ATCC 14579 strain showed 68 genes were upregulated 49 downregulated (>3-fold change). Genes involved cysteine metabolism (putative CymR regulon)...
A novel actinomycete, strain 44EHW(T), was isolated from rhizospheric soil under an Elephant ear plant (Colocasia esculenta) in Bangkok, Thailand. Strain 44EHW(T) produced long branching hyphae and abundant aerial mycelia with chains of rod-shaped spores. Whole-cell hydrolysates contained galactose, glucose, arabinose, ribose, mannose rhamnose as diagnostic sugars. meso-Diaminopimelic acid the diamino glycine, alanine glutamic were present cell-wall peptidoglycan acyl type being acetyl....