Efficient bioconversion of lignocellulosic biomass to bioethanol is one key challenges in the situation increasing demand. The ethanologenic microbes for such conversion are required possess abilities utilization various sugars including xylose and arabinose biomass. As additional characteristics, there a weak or no glucose repression that allows cells simultaneously utilize together with thermotolerance fermentation at high temperatures, which has several advantages reduction cooling cost....

Abstract Background Spathaspora passalidarum is a yeast with the highly effective capability of fermenting several monosaccharides in lignocellulosic hydrolysates, especially xylose. However, this was shown to be sensitive furfural released during pretreatment and hydrolysis processes lignocellulose biomass. We aimed improve tolerance previously isolated S. CMUWF1−2, which presented thermotolerance no detectable glucose repression, via adaptive laboratory evolution (ALE). Results An adapted...

The Bacillus cereus group frequently contaminates milk and dairy products. Some members of this can produce the heat-stable pre-formed toxin cereulide, which causes emetic foodborne intoxication. This study characterised B. isolates from raw cow’s in biochemical, genetic, toxigenic aspects. Of 158 derived 99 samples, 7 (4.43%) harboured cereulide synthetase A (cesA), encodes a associated with phenotype. Heat-treated culture filtrates cesA-positive demonstrated cytotoxicity to HepG2 Caco-2...

Lung cancer remains one of the most lethal malignancies, often diagnosed at advanced stages, leading to limited treatment options. Thus, identifying natural products with potent anti-cancer activity is crucial for improving outcomes. While melittin from Apis mellifera (Mel-AM) has been extensively studied, florea (Mel-AF), a bee species native Southeast Asia, relatively underexplored. These peptides were comparatively investigated against non-small cell lung (NSCLC) lines, A549, NCI-H460,...

Bees play a crucial role as pollinators, contributing significantly to ecosystems. However, the honeybee population faces challenges such global warming, pesticide use, and pathogenic microorganisms. Promoting bee growth using several approaches is therefore for maintaining their roles. To this end, bacterial microbiota well-known its native in supporting respects. Maximizing capabilities of these microorganisms holds theoretical potential promote bees. Recent advancements have made it...

In recent years, researchers have turned their attention to the co-cultivation of microalgae and plants as a means enhance growth hydroponically cultivated while concurrently producing microalgal biomass. However, techniques used require precise calibration based on plant responses interactions with environment cultivation conditions. This study initially focused examining impact hydroponic nutrient concentrations microalga Chlorella sp. AARL G049. The findings revealed that solutions...

Significance Microorganisms can be rationally engineered to convert CO 2 and H O into chemicals, replacing those made from fossil fuels today. Sometimes such chemicals are poorly soluble in water or negatively affect the growth of microorganism, resulting cost-inefficient manufacturing. In nature, this problem is often solved by converting incompatible more compatible with host and/or environment. Inspired this, we propose a similar strategy for biotechnology, whereby biochemical conversion...

Abstract Dengue virus (DENV) infection can lead to severe outcomes through a virus-induced cytokine storm, resulting in vascular leakage and inflammation. An effective treatment strategy should target both replication storm. This study identified Kaempferia galanga L. (KG) extract as exhibiting anti-DENV activity. The major bioactive compound, ethyl-p-methoxycinnamate (EPMC), significantly reduced DENV-2 infection, virion production, viral protein synthesis HepG2 A549 cells, with...

Dengue virus (DENV) infection poses a global health threat, leading to severe conditions with the potential for critical outcomes. Currently, there are no specific drugs available whereas vaccine does not offer comprehensive protection across all DENV serotypes. Therefore, development of antiviral agents is necessary reduce severity risk and interrupt transmission circuit. The search effective against has predominantly focused on natural resources, particularly those demonstrating diverse...

Periodontal disease is more prevalent in patients with diabetes, and it has a negative impact on their quality of life. Inhibiting the infection inflammation processes that cause periodontal can reduce severity chances serious complications. In this study, we aimed to demonstrate effectiveness Clinacanthus nutans extract reducing gingival fibroblast cells induced by Porphyromonas gingivalis lipopolysaccharide (LPS). Stimulation LPS under high-glucose conditions led increased compared...

1-Octanol has gained interest as a chemical precursor for both high and low value commodities including fuel, solvents, surfactants, fragrances. By harnessing the power from sunlight CO2 carbon source, cyanobacteria recently been engineered renewable production of 1-octanol. The productivity, however, remained low. In present work, we report efforts to further improve 1-octanol productivity. Different N-terminal truncations were evaluated on three thioesterases different plant species,...

Heavy metal polluted wastewater from industries is currently one of the major environmental concerns leading to insufficient supply clean water. Several strategies have been implemented overcome this challenge including use microalgae as heavy bio-removers. However, there are still limitations that prevent function optimally. Synthetic biology a new biological discipline developed solve challenging problems via bioengineering approaches. To date, synthetic has no universally affirmed...

In this study, we aim to investigate the efficiency of crude oil bioremediation through composting and culture-assisted composting. First, forty-eight bacteria were isolated from a oil-contaminated soil, isolate with highest degradation activity, identified as Pseudomonas aeruginosa, was selected. The then investigated compared between soil (S), contaminated composted fruit-based waste (SW), same addition selected bacterium (SWB). Both compost-based methods showed high efficiencies (78.1%...

Hydrogen sulfide (H2S) is a toxic and corrosive component that commonly occurs in biogas. In this study, H2S removal from swine-waste biogas using sulfur-oxidizing Paracoccus versutus CM1 immobilized porous glass (PG) polyurethane foam (PUF) biofilters was investigated. Bacterial compositions the were also determined polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The first tested on laboratory scale under three space velocities (SV): 20, 30, 40 h−1. Within 24...

Cyanobacteria and algae serving as promising food supplements have recently garnered attention for their emerging potential in anti-cancer activity. Cholangiocarcinoma (CCA) or bile duct cancer is one of the top-leading cancers affecting people, particularly Asian continent. With patients exhibiting no minimal symptoms early stages, advanced CCA often diagnosed, primary treatments such surgery may not be suitable. Discovery natural bioactive compounds have, thus, attracted effective means to...

This study investigates the therapeutic potential of

Dengue virus (DENV) infection has emerged as a global health problem, with no specific treatment available presently.

Abstract Defining suitable enzymes for reaction steps in novel synthetic pathways is crucial developing microbial cell factories non-natural products. Here, we developed a computational workflow to identify C12 alcohol-active UDP-glycosyltransferases. The involved three steps: (1) assembling initial candidates of putative UDP-glycosyltransferases, (2) refining selection by examining conserved regions, and (3) 3D structure prediction molecular docking. Genomic sequences from Candida , Pichia...

Microalgae have emerged as a promising, next-generation sustainable resource with versatile applications, particularly expression platforms and green cell factories. They possess the ability to overcome limitations of terrestrial plants, such non-arable land, water scarcity, time-intensive growth, seasonal changes. However, heterologous interested genes in microalgae under heterotrophic cultivation (dark mode) remains niche area within field engineering technologies. In this study,...

Synthetic biology is a principle that aims to create new biological systems with particular functions or redesign the existing ones through bioengineering. Therefore, this often utilized as tool put knowledge learned practical use in actual fields. However, there still great deal of information remaining be found, and limits possible utilization synthetic biology, particularly on topic focus present work-heavy metal bio-removal. In work, we aim construct comprehensive library putative...