A5085826127- Microbial Natural Products and Biosynthesis
- Fungal Biology and Applications
- Genomics and Phylogenetic Studies
- Marine Sponges and Natural Products
- Synthetic Organic Chemistry Methods
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Microbial Metabolism and Applications
- Plant Pathogens and Fungal Diseases
- Carbohydrate Chemistry and Synthesis
- Bacterial Genetics and Biotechnology
- Phytochemical compounds biological activities
- Plant biochemistry and biosynthesis
- Chemical Synthesis and Analysis
- Bioactive Compounds and Antitumor Agents
- Synthesis and bioactivity of alkaloids
- Microbial Metabolic Engineering and Bioproduction
- Advanced Synthetic Organic Chemistry
- RNA and protein synthesis mechanisms
- Amino Acid Enzymes and Metabolism
- Cancer therapeutics and mechanisms
- Enzyme Structure and Function
- Porphyrin Metabolism and Disorders
- Steroid Chemistry and Biochemistry
- Alkaloids: synthesis and pharmacology
Aoyama Gakuin University
2025
Osaka University
2012-2021
Osaka International University
2007-2021
Kitasato University
2012
Gram-positive bacteria of the genus Streptomyces are industrially important microorganisms, producing >70% commercially antibiotics. The production these compounds is often regulated by low-molecular-weight bacterial hormones called autoregulators. Although 60% strains may use γ-butyrolactone–type molecules as autoregulators and some furan-type molecules, little known about signaling used to regulate antibiotic in many other members this genus. Here, we purified a molecule (avenolide)...
Kitasatospora setae NBRC 14216T (=KM-6054T) is known to produce setamycin (bafilomycin B1) possessing antitrichomonal activity. The genus morphologically similar the Streptomyces, although they are distinguishable from each other on basis of cell wall composition and 16S rDNA sequence. We have determined complete genome sequence K. as first Streptomycetaceae than Streptomyces. a single linear chromosome 8 783 278 bp with terminal inverted repeats 127 148 bp, predicted encode 7569...
Abstract Protein kinase C (PKC) is a family of serine/threonine kinases, and PKC ligands have the potential to be therapeutic seeds for cancer, Alzheimer's disease, human immunodeficiency virus infection. However, in addition desired effects, most also exhibit undesirable pro-inflammatory effects. The discovery new scaffolds important developing less inflammatory ligands, such as bryostatins. We previously reported that machine learning combined with our knowledge pharmacophore yielded 15...
ABSTRACT Polyene macrolide antibiotics, produced mainly as secondary metabolites of streptomycetes, have distinct chemical structures and include clinically important antifungal drugs. We recently isolated the 28-membered polyene lavencidin from Streptomyces lavendulae FRI-5. Here, we identify characterize biosynthetic ( lad ) gene cluster by combining a disruption system based on base editing technology in silico analysis. Sequence analysis draft genome S. FRI-5 revealed plausible genes,...
IM-2 [(2R,3R,1'R)-2-1'-hydroxybutyl-3-hydroxymethyl gamma-butanolide] is a gamma-butyrolactone autoregulator which, in Streptomyces lavendulae FRI-5, switches off the production of D-cycloserine but on blue pigment and several nucleoside antibiotics. To clarify vivo function an IM-2-specific receptor (FarA) signaling cascade S. farA deletion mutant was constructed by means homologous recombination. On solid media, no significant difference morphology observed between wild-type strain (strain...
The γ-butyrolactone autoregulator signaling cascades have been shown to control secondary metabolism and/or morphological development among many Streptomyces species. However, the conservation and variation of regulatory systems actinomycetes remain be clarified. genome sequence Kitasatospora setae, which also belongs family Streptomycetaceae containing genus Streptomyces, has revealed presence three homologues receptor: KsbA, previously confirmed involved only in metabolism; KsbB; KsbC. We...
In streptomycetes, autoregulators are important signaling compounds that trigger secondary metabolism, and they regarded as Streptomyces hormones based on their extremely low effective concentrations (nM) the involvement of specific receptor proteins. Our previous distribution study revealed butenolide-type hormones, including avenolide, a general class molecules in streptomycetes albus strain J1074 may produce hormones. Here, we describe metabolite profiling disruptant S. albusaco gene,...
The γ-butyrolactone autoregulator receptor has been shown to control secondary metabolism and/or morphological differentiation across many Streptomyces species. avermitilis produces an important anthelmintic agent (avermectin) and two further polyketide antibiotics, filipin oligomycin. Genomic analysis of S. revealed that this micro-organism the clustered putative genes distant from antibiotic biosynthetic gene clusters. Here, we describe characterization avaR3 , one genes, which encodes a...
ABSTRACT FarA of Streptomyces lavendulae FRI-5 is a specific receptor protein for IM-2, butyrolactone autoregulator that controls the production blue pigment and nucleoside antibiotics showdomycin minimycin. Gel shift assays demonstrated binds to farA upstream region this binding abolished in presence IM-2. The sequence was localized by DNase I footprinting 28-bp located approximately 70 bp translational start site. High-resolution S1 nuclease mapping transcripts revealed putative...
Two regulatory genes encoding a Streptomyces antibiotic protein ( vmsS ) and response regulator vmsT of bacterial two-component signal transduction system are present in the left-hand region biosynthetic gene cluster virginiamycin, which is composed virginiamycin M (VM) S (VS), virginiae . Disruption abolished both VM VS biosynthesis, with drastic alteration transcriptional profile for genes, whereas disruption resulted only loss suggesting that pathway-specific biosynthesis alone. Gene...
Virginiae butanolide (VB) is a gamma-butyrolactone autoregulator that triggers production of the streptogramin antibiotic virginiamycin in Streptomyces virginiae. Our previous studies suggested barX gene, an afsA-family likely to participate regulatory pathway for VB, rather than biosynthetic VB itself, contrast function other genes. Mutation analysis now shows BarX at least plays enzymic role pathway. Heterologous expression afsA gene from griseus into mutant partially restored deficiency...
Jomthonic acid A (1), a new modified amino acid, was isolated from the culture broth of soil-derived actinomycete genus Streptomyces. The structure and absolute configuration 1 were determined by spectroscopic analyses chemical conversion. (1) induced differentiation preadipocytes into mature adipocytes at 2–50 μM.
Phthoxazolin A, an oxazole-containing polyketide, has a broad spectrum of anti-oomycete activity and herbicidal activity. We recently identified phthoxazolin A as cryptic metabolite Streptomyces avermitilis that produces the important anthelmintic agent avermectin. Even though genome data S. is publicly available, no plausible biosynthetic gene cluster for apparent in sequence data. Here, we characterized (ptx) through sequencing, comparative genomic analysis, disruption. Sequence analysis...