A5035146034- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Methane Hydrates and Related Phenomena
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Pancreatic and Hepatic Oncology Research
- Amino Acid Enzymes and Metabolism
- Enzyme Structure and Function
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Microbial Metabolic Engineering and Bioproduction
- Gallbladder and Bile Duct Disorders
- Heat shock proteins research
- Immune cells in cancer
- Advanced biosensing and bioanalysis techniques
- Environmental DNA in Biodiversity Studies
- Plant Disease Resistance and Genetics
- Polyamine Metabolism and Applications
- Bee Products Chemical Analysis
- CRISPR and Genetic Engineering
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Nutrition and Health in Aging
- Lipid metabolism and biosynthesis
- Anaerobic Digestion and Biogas Production
- Chronic Lymphocytic Leukemia Research
- Protist diversity and phylogeny
Osaka University
2019-2025
Yamaguchi University
2024
Tohoku University Hospital
2024
Tokyo Metropolitan Tama-Hokubu Medical Center
2024
Osaka International University
2020-2022
Shizuoka University
2014-2020
Graduate School USA
2014
Growth temperature is one of the most representative biological parameters for characterizing living organisms. Prokaryotes have been isolated from various environments and show wide diversity in their growth temperatures. We herein constructed a database TEMPeratures Usual RAre prokaryotes (TEMPURA, http://togodb.org/db/tempura), which contains minimum, optimum, maximum temperatures 8,639 prokaryotic strains. information linked with taxonomy IDs, phylogenies, genomic information. TEMPURA...
Microbiome engineering is an emerging research field that aims to design artificial microbiome and modulate its function. In particular, subtractive modification of the allows us create without microorganism interest evaluate functions interactions with other constituent bacteria. However, few techniques can specifically remove only a single species from large number microorganisms be applied universally variety have been developed. Antisense peptide nucleic acid (PNA) potent designable...
Abstract Small heat shock proteins (HSPs), such as HSP20, represent cellular thermal resistance mechanisms, to avoid protein aggregation at elevated temperatures. Recombinantly expressed HSP20s serve a molecular tool for improving the tolerance of living cells various physical and chemical stressors. Here, we aimed heterologously express 18 from 12 thermotolerant bacteria in Escherichia coli evaluate their effects on stresses. Seventeen were successfully soluble proteins. Recombinant E....
Abstract Polyphosphate kinase 2 (PPK2) transfer phosphate from inorganic polyphosphate to nucleotides. According their activity, PPK2 enzymes are classified into three groups. Among them, class III catalyze both the phosphorylation of nucleotide mono‐ diphosphates and di‐ triphosphates by using polyphosphate, which is a very inexpensive substrate. Therefore, attractive for use in biotechnological applications. Despite several studies on enzymes, detailed mechanism how transferred remains be...
There has been an increasing demand for optically pure d-lactic and l-lactic acid the production of stereocomplex-type polylactic acid. The from lignocellulosic biomass is important owing to its great abundance in nature. Corn steep liquor (CSL) a cheap nitrogen source used industrial fermentation, though it contains significant amount acid, which decreases optical purity produced.To remove derived CSL-based medium, l-lactate oxidase (LoxL) Enterococcus sp. NBRC 3427 was expressed engineered...
In natural microbiomes, microorganisms interact with each other and exhibit diverse functions. Microbiome engineering, which enables bacterial knockdown, is a promising method to elucidate the functions of targeted bacteria in microbiomes. However, few methods selectively kill target microbiome without affecting growth nontarget are available. this study, we focused on host-specific lytic ability virulent phages validated their potency for precise engineering. an artificial consisting...
Summary In a deep aquifer associated with an accretionary prism, significant methane ( CH 4 ) is produced by subterranean microbial community. Here, we developed bioreactors for producing and hydrogen H 2 using anaerobic groundwater collected from the aquifer. To generate , amended organic substrates was incubated in bioreactor. At first, detected accumulated gas phase of After decreased, rapid production observed. Phylogenetic analysis targeting 16 S rRNA genes revealed that ‐producing...
The halophilic archaeon Haloarcula hispanica harbors three ribosomal RNA (rRNA) operons (rrnA, rrnB, and rrnC) that contain the 16S rRNA genes rrsA, rrsB, rrsC, respectively. Although rrsB rrsC (rrsBC) have almost identical sequences, rrsA rrsBC sequences differ by 5.4%, they 2.5% with respect to guanine-plus-cytosine content (PGC). strong correlation between typical growth temperatures of archaea PGC their suggests Har. may harbor different having maintain rapid in a wide range...
Abstract Cancer cachexia, a paraneoplastic syndrome characterized by ongoing skeletal muscle mass loss, is accompanied adipose tissue loss and strongly affects chemotherapy endurance. Our aim was to detect serum marker reflecting pancreatic cancer cachexia predicting subsequent of tissue, focusing on tissue‐secreted proteins. Murine‐derived cells were orthotopically injected into the mouse tail. After 3 weeks, RNA sequencing perigonadal fat orthotopic tumors carried out. We analyzed stocked...
Accretionary prisms are thick masses of sedimentary material scraped from the oceanic crust and piled up at convergent plate boundaries found across large regions world. Large amounts anoxic groundwater natural gas, mainly methane (CH4), contained in deep aquifers associated with these accretionary prisms. To identify subsurface environments potential for CH4 production by microbial communities aquifers, we performed chemical microbiological assays on gas derived an prism its overlying...
S-Adenosylmethionine (SAM)-dependent methyltransferases are important tools for the biocatalytic methylation of diverse biomolecules. Methylation by a whole-cell biocatalyst allows utilization intrinsic SAM and its regeneration system, which consists cyclic multi-step enzymatic cascade. However, low intracellular availability 5-methyl-tetrahydrofolate (5-methyl-THF), functions as methyl group donor, limits regeneration. Here, we integrated methanol metabolism with 5-methyl-THF formation into...
Accretionary prisms are thick layers of sedimentary material piled up at convergent plate boundaries. Large amounts anaerobic groundwater and methane (CH4) contained in the deep aquifers associated with accretionary prisms. In order to identify microbial activity CH4 production processes Cretaceous prism Okinawa Island, Japan, we performed geochemical microbiological studies using natural gas (mainly CH4) samples collected through four wells. Chemical stable hydrogen oxygen isotope analyses...
Abstract Background Pancreatitis is known to be an important risk factor for pancreatic ductal adenocarcinoma (PDAC). However, the exact molecular mechanisms of how inflammation promotes PDAC are still not fully understood. Regnase-1, endoribonuclease, regulates immune responses by degrading mRNAs inflammation-related genes. Herein, we investigated role Regnase-1 in PDAC. Methods Clinical significance intratumor expression was evaluated immunohistochemistry 39 surgically-resected patients....
Haloarcula strains, which are halophilic archaea, harbour two to three copies of 16S rRNA genes (rrsA, rrsB and rrsC) in their genomes. While rrsC (rrsBC) show almost identical sequences, rrsA shows 4–6% sequence difference 1–3% guanine-plus-cytosine content (PGC) compared rrsBC. Based on the strong correlation between PGC growth temperatures prokaryotes, we hypothesised that high-PGCrrsA low-PGCrrsBC expressed at high low temperatures, respectively. To verify hypothesis, performed analyses...
Cytochrome P450 2B6 (CYP2B6) catalyzes the metabolism of many drugs, including efavirenz and propofol. Genetic polymorphisms in CYP2B6 alter its enzymatic activity substantially affect pharmacokinetics. High-frequency variants, such as CYP2B6*6, are associated with risk developing side effects due to reduced activity. However, impact rare alterations on enzyme function remains unknown, some these variants may significantly decrease Therefore, this study, we evaluated vitro functional 29...
Accretionary prisms are composed mainly of ancient marine sediment scraped from the subducting oceanic plate at convergent boundaries. Anoxic groundwater is stored in deep aquifers associated with accretionary and can be collected via wells. We investigated how such pumping affects microbial community a aquifer. Groundwater samples were well drilled down to 1500 m every six months (five times total) after completion construction start pumping. Next-generation sequencing clone-library...
Cytochrome P450 2B6 (CYP2B6) catalyzes the metabolism of many drugs, including efavirenz (EFZ) and propofol (PRF). Genetic polymorphisms in CYP2B6 alter its enzymatic activity substantially affect pharmacokinetics. High-frequency variants, such as CYP2B6*6, are associated with risk developing side effects due to reduced activity. However, impact rare alterations on enzyme function remains unknown, some these variants may significantly decrease Therefore, this study, we evaluated functional...
Perihilar cholangiocarcinoma (PCC) is a complex disorder involving the hepatic hilum. Multiple endoscopic retrograde cholangiopancreatography sessions are necessary for diagnosis and treatment with underlying cholangitis risk. Our aim to clarify initial-drainage-related prognostic factors of PCC.This study was single-center retrospective study. A total 104 consecutive patients diagnosed PCC from January 2010 February 2020 were enrolled. We defined diagnostic period as time between first...