A5015381194- Antibiotic Resistance in Bacteria
- Drug Transport and Resistance Mechanisms
- Cancer therapeutics and mechanisms
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Genetics and Biotechnology
- Genetic and phenotypic traits in livestock
- Bacterial biofilms and quorum sensing
- Trace Elements in Health
- Phenothiazines and Benzothiazines Synthesis and Activities
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial infections and disease research
- Infections and bacterial resistance
- Antimicrobial Resistance in Staphylococcus
- Iron Metabolism and Disorders
- Antibiotic Use and Resistance
- Antimicrobial Peptides and Activities
- Reproductive Physiology in Livestock
- Essential Oils and Antimicrobial Activity
- Tuberculosis Research and Epidemiology
- Bacteriophages and microbial interactions
- Listeria monocytogenes in Food Safety
- Amino Acid Enzymes and Metabolism
- DNA and Nucleic Acid Chemistry
- Pneumonia and Respiratory Infections
- Enzyme Structure and Function
University of South Australia
2016-2025
The University of Queensland
2024
University of Cambridge
2003-2012
University of Leeds
1999-2004
University of the Free State
1999-2002
University of Pretoria
1973-1980
Drug efflux pumps confer resistance upon bacteria to a wide range of antibiotics from various classes. The expression are also implicated in virulence and biofilm formation. Moreover, organisms can only acquire the presence active drug pumps. Therefore, pump inhibitors (EPIs) attractive compounds reverse multidrug prevent development clinically relevant bacterial pathogens. We investigated potential pure isolated plants act as EPIs. In silico screening was used predict bioactivity plant...
Pseudomonas aeruginosa is an opportunistic pathogen displaying high intrinsic antimicrobial resistance and the ability to thrive in different ecological environments. In this study, of P. develop simultaneous multiple antibiotics disinfectants natural niches were investigated using strains collected from clinical samples, veterinary wastewater. The correlation between biocide was determined by employing principal component analysis. Molecular mechanisms linking interrogated determining gene...
The human breast cancer resistance protein (BCRP, also know as ABCG2, MXR, or ABCP) is one of the more recently discovered ATP-binding cassette (ABC) transporters that confer on cells by mediating multidrug efflux. In present study, we have obtained functional expression BCRP in Gram-positive bacterium Lactococcus lactis. conferred lactococcal cells, which was based ATP-dependent drug extrusion. BCRP-mediated ATPase and transport activities were inhibited BCRP-specific modulator...
LmrA is an ATP binding cassette (ABC) multidrug transporter in Lactococcus lactis that a structural and functional homologue of the human resistance P-glycoprotein MDR1 (ABCB1). also homologous to MsbA, essential ABC Escherichia coli involved trafficking lipids, including Lipid A. We have compared substrate specificities MsbA detail. Surprisingly, was able functionally substitute for temperature-sensitive mutant E. WD2 at non-permissive temperatures, suggesting could transport exhibited...
The spread of multidrug resistance among bacterial pathogens poses a serious threat to public health worldwide. Recent approaches towards combating antimicrobial include repurposing old compounds with known safety and development pathways as new antibacterial classes novel mechanisms action. Here we show that an analog the anticoccidial drug robenidine (4,6-bis(2-((E)-4-methylbenzylidene)hydrazinyl)pyrimidin-2-amine; NCL195) displays potent bactericidal activity against Streptococcus...
Klebsiella pneumoniae is a Gram-negative pathogen that has become worldwide concern due to the emergence of multidrug-resistant isolates responsible for various invasive infectious diseases. Biofilm formation constitutes major virulence factor K. and relies on expression fimbrial adhesins aggregation bacterial cells biotic or abiotic surfaces in coordinated manner. During biofilm aggregation, communicate with each other through inter- intra-species interactions mediated by signallng...
The emergence of macrolide and tetracycline resistance within Pasteurella multocida isolated from feedlot cattle the dominance ST394 in Australia was reported recently.
The human BCRP (breast cancer resistance protein, also known as ABCG2) is an ABC (ATP-binding cassette) transporter that extrudes various anticancer drugs from cells, causing multidrug resistance. To study the molecular determinants of drug specificity in more detail, we have expressed wild-type (BCRP-R) and drug-selected cell line-associated R482G (Arg482→Gly) mutant (BCRP-G) Lactococcus lactis. Drug rate efflux BCRP-expressing cells were proportional to expression level protein affected by...
MsbA is an essential ATP-binding cassette half-transporter in the cytoplasmic membrane of gram-negative Escherichia coli and required for export lipopolysaccharides (LPS) to outer membrane, most likely by transporting lipid A core moiety. Consistent with homology multidrug transporter LmrA gram-positive Lactococcus lactis, our recent work E. suggested that might interact multiple drugs. To enable a more detailed analysis transport environment deficient LPS, we functionally expressed L....
The Stenotrophomonas maltophilia L2 cephalosporinase is one of two beta-lactamases which afford S. beta-lactam resistance. With the overuse beta-lactams, selective pressures have contributed to evolution these proteins, generating proteins with an extended spectrum activity. Variant cephalosporinases been detected, as has their distribution into main clades (Clade 1 and 2). Comprehensive analysis six variants, cloned pET41a(+) expressed in Escherichia coli BL21(DE3) cells, revealed that...
Antimicrobial resistance (AMR) poses a global threat to public health. The excessive use of antibiotics significantly contributed the rise resistance. Recent evidence suggests that non-antibiotic medications (NAMs) also play role in antimicrobial development, although this aspect remains less explored and understood. This issue is particularly relevant residential aged care facilities (RACFs) where both NAM are frequently used, AMR prevalent. We investigated propensity NAMs commonly used...
Discovering new bacterial signaling pathways offers unique antibiotic strategies. With current classes targeting cell wall synthesis or depolarizing the inner-membrane altering metabolome inhibiting replication transcription pathways, manipulation of transporters to limit respiration and thereby pathogenesis has been a decades long quest. Here we report an inhibitor multiple transporters. The is bactericidal N-104 endogenous cleavage fragment prosecretory mitogen lacritin. Lacritin now known...
Carbapenem-resistant Pseudomonas aeruginosa (CRPA) is a significant opportunistic human pathogen, posing considerable threat to public health due its antimicrobial resistance and limited treatment options. The incidence of CRPA high in the Philippines; however, genomic analysis this setting limited. Here, we provide phenotypic molecular characterization 35 non-duplicate obtained from three tertiary hospitals Metro Manila, Philippines, August 2022 January 2023. Six sequence types (STs),...