A5000015504- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- Bacterial Genetics and Biotechnology
- Clostridium difficile and Clostridium perfringens research
- Microbial infections and disease research
- Plant Pathogenic Bacteria Studies
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Vibrio bacteria research studies
- Enterobacteriaceae and Cronobacter Research
- Plant-Microbe Interactions and Immunity
- Mycorrhizal Fungi and Plant Interactions
- Aquaculture disease management and microbiota
- Viral gastroenteritis research and epidemiology
- Plant Pathogens and Fungal Diseases
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Gut microbiota and health
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial Identification and Susceptibility Testing
- Botulinum Toxin and Related Neurological Disorders
- Urinary Tract Infections Management
- Microbial Metabolism and Applications
University of Technology Sydney
2015-2024
New South Wales Department of Primary Industries
2013-2019
Macquarie University
2008-2013
University of Technology
2012
University of Canterbury
2006-2007
Multiple antibiotic resistant pathogens represent a major clinical challenge in both the human and veterinary context. It is now well understood that genes encode resistance are context independent. That is, same gene commonly present otherwise very disparate humans production companion animals, among bacteria proliferate an agricultural This can be true even for pathogenic species or clonal types confined to single host ecological niche. therefore follows mechanisms of flow must exist move...
Avian pathogenic Escherichia coli (APEC) cause widespread economic losses in poultry production and are potential zoonotic pathogens. Genome sequences of 95 APEC from commercial operations four Australian states that carried the class 1 integrase gene intI1, a proxy for multiple drug resistance (MDR), were characterized. Sequence types ST117 (22/95), ST350 (10/95), ST429 ST57 (each 9/95), ST95 (8/95) ST973 (7/95) dominated, while 24 STs represented by one or two strains. FII FIB repA genes...
Abstract Even in the setting of optimal resuscitation high-income countries severe sepsis and septic shock have a mortality 20–40%, with antibiotic resistance dramatically increasing this risk. To develop reference dataset enabling identification common bacterial targets for therapeutic intervention, we applied standardized genomic, transcriptomic, proteomic metabolomic technological framework to multiple clinical isolates four sepsis-causing pathogens: Escherichia coli , Klebsiella...
Porcine faecal waste is a serious environmental pollutant. Carriage of antimicrobial-resistance genes (ARGs) and virulence-associated (VAGs), the zoonotic potential commensal Escherichia coli from swine are largely unknown. Furthermore, little known about role E. as contributors to mobilization ARGs between food animals environment. Here, we report whole-genome sequence analysis 103 class 1 integron-positive faeces healthy pigs two commercial production facilities in New South Wales,...
The emergence of macrolide and tetracycline resistance within Pasteurella multocida isolated from feedlot cattle the dominance ST394 in Australia was reported recently.
A comparative genetic analysis of 42 clinical Klebsiella pneumoniae isolates, resistant to two or more antibiotics belonging the broad-spectrum β-lactam group, sourced from Sydney, Australia, and three South American countries is presented. The study focuses on contexts class 1 integrons, mobilizable elements best known for their role in rapid evolution antibiotic resistance among Gram-negative pathogens. It was found that integrons this cohort were located a number different with clear...
Enterohemorrhagic Escherichia coli (EHEC) and atypical enteropathogenic E. (aEPEC) are important zoonotic pathogens that increasingly becoming resistant to multiple antibiotics. Here we describe two plasmids, pO26-CRL125 (125 kb) from a human O26:H- EHEC, pO111-CRL115 (115kb) bovine O111 aEPEC, impart resistance ampicillin, kanamycin, neomycin, streptomycin, sulfathiazole, trimethoprim tetracycline both contain class 1 integrons with an identical IS26-mediated deletion in their 3´-conserved...
Pseudomonas aeruginosa are noscomially acquired, opportunistic pathogens that pose a major threat to the health of burns patients and immunocompromised. We sequenced genomes P. isolates RNS_PA1, RNS_PA46 RNS_PAE05, which displayed resistance almost all frontline antibiotics, including gentamicin, piperacillin, timentin, meropenem, ceftazidime colistin. provide evidence representatives sequence type (ST) 235 carry Tn6162 Tn6163 in genomic islands 1 (GI1) 2 (GI2), respectively. GI1 disrupts...
Escherichia coli ST405 is an emerging urosepsis pathogen, noted for carriage of blaCTX-M, blaNDM, and a repertoire virulence genes comparable with O25b:H4-ST131. Extraintestinal multidrug resistant E. are poorly studied in Australia. Here we determined the genome sequence uropathogenic, multiple drug (strain 2009-27) from mid-stream urine hospital patient Sydney, Australia, using combination Illumina SMRT sequencing. The strain 2009-27 assembled into two unitigs; chromosome comprising...
Escherichia coli ST131 is a globally dispersed extraintestinal pathogenic E. lineage contributing significantly to hospital and community acquired urinary tract bloodstream infections. Here we describe detailed phylogenetic analysis of the whole genome sequences 284 Australian isolates from diverse sources, including clinical, food companion animals, wildlife environment. Our phylogeny results single nucleotide polymorphism (SNP) show typical clade distribution with clades A, B C clearly...
Bacterial aminopeptidases play important roles in pathogenesis by providing a source of amino acids from exogenous proteins, destroying host immunological effector peptides and executing posttranslational modification bacterial proteins. We show that MHJ_0125 the swine respiratory pathogen Mycoplasma hyopneumoniae represents new member M42 class aminopeptidases. Despite lacking recognizable signal sequence, is detectable on cell surface fluorescence microscopy LC-MS/MS (i) biotinylated...
Full genome sequences of 20 strains Clostridium chauvoei, the etiological agent blackleg cattle and sheep, isolated from four different continents over a period 64 years (1951-2015) were determined analyzed. The study reveals that species C. chauvoei is highly homogeneous compared to closely-related perfringens, widespread pathogen affects human many animal species. Analysis CRISPR locus sufficient differentiate most heterogenous region in genome, containing total 187 spacer elements are...
ABSTRACT A class 2 integron was found in an Escherichia coli isolate mediating a urinary tract infection. Unlike other integrons from pathogens, the encoded IntI2 protein functional. The possessed dfrA14 cassette, and second novel cassette which lipoprotein signal peptidase gene is predicted.
ABSTRACT Eleven clinical class 1 integron-containing Pseudomonas aeruginosa isolates from Australia and Uruguay were investigated for the genomic locations of these elements. Several novel integrons/transposons found in at least four distinct chromosome, including islands. These elements seem to be undergoing successful dispersal by lateral gene transfer since integrons identified across several lineages more than one clonal line.
Enterobacter hormaechei is an important emerging pathogen and a key member of the highly diverse cloacae complex. E. strains can persist spread in nosocomial environments, often exhibit resistance to multiple clinically antibiotics. However, genomic regions that harbour determinants are typically repetitive impossible resolve with standard short-read sequencing technologies.Here we used both short- long-read methods sequence genome multidrug-resistant hospital isolate (C15117), which...
This study sought to assess the genetic variability of Escherichia coli isolated from bloodstream infections (BSIs) presenting at Concord Hospital, Sydney during 2013–2016. Whole-genome sequencing was used characterize 81 E. isolates sourced community-onset (CO) and hospital-onset (HO) BSIs. The cohort comprised 64 CO 17 HO isolates, including 35 multidrug-resistant (MDR) exhibiting phenotypic resistance three or more antibiotic classes. Phylogenetic analysis identified two major ancestral...
In a survey of class 1 integrons from human stools, an unusual integron strain Enterobacter cloacae was isolated and characterized in detail. Sequence analysis fosmid containing the revealed complex set transposons which included two Tn402-like transposons. One these transposons, Tn6007, with non-antibiotic-resistance-type gene cassettes complete transposition module. This tni module is hybrid boundary within res site compared to Tn402, implying that site-specific recombination event...
Enterotoxigenic Escherichia coli (ETEC) are a major economic threat to pig production globally, with serogroups O8, O9, O45, O101, O138, O139, O141, O149 and O157 implicated as the leading diarrhoeal pathogens affecting pigs below four weeks of age. A multiple antimicrobial resistant ETEC (O157 SvETEC) representative isolates from farm in New South Wales, Australia that experienced repeated bouts pre- post-weaning diarrhoea resulting fatalities was characterized here. Enterohaemorrhagic E....
In enteroaggregative hemorrhagic Escherichia coli (EAHEC) O104 the complex antibiotic resistance gene loci (CRL) found in region of divergence 1 (RD1) within E. genomic island 3 (GI3) contains blaTEM-1, strAB, sul2, tet(A)A, and dfrA7 genes encoding to ampicillin, streptomycin, sulfamethoxazole, tetracycline trimethoprim respectively. The precise arrangement role mobile elements that drove evolutionary events created CRL have not been investigated. We used a combination bioinformatics...
ABSTRACT Cavity disease in white button mushrooms is caused by Burkholderia gladioli pv. agaricicola. We describe the isolation and characterization of six mutants strain BG164R that no longer cause this on mushrooms. The mutations were mapped to genes general secretory pathway (GSP). This first report association type II secretion with a Phenotypes avirulent following: an inability degrade mushroom tissue, highly reduced capacity secrete chitinase protease, number flagella. Using these...