A5089681901- Metabolomics and Mass Spectrometry Studies
- Scientific Computing and Data Management
- Environmental Toxicology and Ecotoxicology
- Genetics, Bioinformatics, and Biomedical Research
- Research Data Management Practices
- Mosquito-borne diseases and control
- Advanced Proteomics Techniques and Applications
- Microbial infections and disease research
- Enzyme Structure and Function
- Genomics, phytochemicals, and oxidative stress
- Bacteriophages and microbial interactions
- Protein Structure and Dynamics
- Insect symbiosis and bacterial influences
- Aquaculture disease management and microbiota
- Machine Learning in Bioinformatics
- Analytical Chemistry and Chromatography
- Vector-borne infectious diseases
- Physiological and biochemical adaptations
- Lung Cancer Treatments and Mutations
- Advanced Chemical Sensor Technologies
- Biomedical Text Mining and Ontologies
- Aquaculture Nutrition and Growth
- Adipokines, Inflammation, and Metabolic Diseases
- Glutathione Transferases and Polymorphisms
- Fungal and yeast genetics research
The University of Melbourne
2015-2023
Melbourne Genomics Health Alliance
2015-2023
RMIT University
2004-2006
Victoria University
2004
Abstract Even in the setting of optimal resuscitation high-income countries severe sepsis and septic shock have a mortality 20–40%, with antibiotic resistance dramatically increasing this risk. To develop reference dataset enabling identification common bacterial targets for therapeutic intervention, we applied standardized genomic, transcriptomic, proteomic metabolomic technological framework to multiple clinical isolates four sepsis-causing pathogens: Escherichia coli , Klebsiella...
Abstract Competition between viruses and Wolbachia for host lipids is a proposed mechanism of -mediated virus blocking in insects. Yet, the metabolomic interaction symbiont within mosquito has not been clearly defined. We compare lipid profiles Aedes aegypti mosquitoes bearing mono- or dual-infections w Mel strain dengue serotype 3 (DENV3). found metabolic signatures infection-induced intracellular events but little evidence to support direct competition lipids. Lipid dual-infected resemble...
Mass spectrometry (MS) is one of the primary techniques used for large-scale analysis small molecules in metabolomics studies. To date, there has been little data format standardization this field, as different software packages export results formats represented XML or plain text, making sharing, database deposition, and reanalysis highly challenging. Working within consortia Metabolomics Standards Initiative, Proteomics Society, we have created mzTab-M to act a common output from...
Significance Wolbachia ( w Mel strain)-infected Aedes aegypti mosquitoes are refractory to disseminated arboviral infections. Yet previous studies into the mechanism behind -mediated virus blocking have not considered involvement of lipids, apart from cholesterol, during superinfection. We used liquid chromatography mass spectrometry study lipidome in mosquito cells infected with virus, Mel, or superinfected both and Mel. Interestingly, a class acyl-carnitines increased infection but...
Cancer-related and primary lymphedema (LE) are associated with the production of adipose tissue (AT). Nothing is known, however, about lipid-based molecules that comprise LE AT. We therefore analyzed lipid in lipoaspirates serum obtained from patients, compared them to cosmetic surgery patients healthy control cohort serum. patient analysis demonstrated triglycerides, HDL- LDL-cholesterol transport remained within normal range, no alterations individual fatty acids. The lipidomic also...
<ns4:p>Throughout history, the life sciences have been revolutionised by technological advances; in our era this is manifested advances instrumentation for data generation, and consequently researchers now routinely handle large amounts of heterogeneous digital formats. The simultaneous transitions towards biology as a science ‘life cycle’ view research pose new challenges. Researchers face bewildering landscape management requirements, recommendations regulations, without necessarily being...
Mycoplasmas are simple, but successful parasites that have the smallest genome of any free-living cell and thought to a highly streamlined cellular metabolism. Here, we undertaken detailed metabolomic analysis two species, Mycoplasma bovis gallisepticum, which cause economically important diseases in cattle poultry, respectively. Untargeted gas chromatography-mass spectrometry liquid analyses mycoplasma metabolite extracts revealed significant differences steady-state levels many metabolites...
Throughout history, the life sciences have been revolutionised by technological advances; in our era this is manifested advances instrumentation for data generation, and consequently researchers now routinely handle large amounts of heterogeneous digital formats. The simultaneous transitions towards biology as a science 'life cycle' view research pose new challenges. Researchers face bewildering landscape management requirements, recommendations regulations, without necessarily being able to...
Fungi are adept at occupying specific environmental niches and often exploit numerous secondary metabolites generated by the cytochrome P450 (CYP) monoxygenases. This report describes characterization of a yeast-specific CYP encoded simA ("survival in macrophages"). Deletion does not affect yeast growth 37°C vitro but is essential for cell production during macrophage infection. The ΔsimA strain exhibits reduced conidial germination intracellular macrophages, suggesting that enzymatic...
An increasing number of research laboratories and core analytical facilities around the world are developing high throughput metabolomic data processing pipelines that capable handling hundreds to thousands individual samples per year, often over multiple projects, collaborations sample types. At present, there no Laboratory Information Management Systems (LIMS) specifically tailored for metabolomics tracking associated metadata from beginning end an experiment, including archiving, which...
Dihedral angles of amino acids are considerable importance in protein tertiary structure prediction as they define the backbone a and hence almost protein's entire conformation.Most ab initio methods predict secondary before predicting because three-dimensional fold consists repeating units structures.Hence, both dihedral structures important proteins.Here we describe database called DASSD (Dihedral Angle Secondary Structure Database Short Amino acid Fragments) that contains angle values...
Environmental pollutants such as heavy metals and fungicides pose a serious threat to waterways worldwide. Toxicological assessment of contaminants is usually conducted using single compound exposures, it challenging understand the effect mixtures on biota standard ecotoxicological methods; whereas complex chemical are more probable in ecosystems. This study exposed Simplisetiaaequisetis (an estuarine annelid) sublethal concentrations metal (zinc) fungicide (boscalid), both singly mixture,...
This exploratory study aims to investigate the health of sand flathead (Platycephalus bassensis) sampled from five sites in Port Phillip Bay, Australia using gas chromatography-mass spectrometry (GC-MS) metabolomics approaches. Three were recipients industrial, agricultural, and urban run-off considered sites, while remaining two remote contaminant inputs, hence classed as rural sites. Morphological parameters well polar free fatty acid metabolites used inter-site differences fish health....
Abstract Coxiella burnetii is a Gram-negative bacterium which causes Q fever, complex and life-threatening infection with both acute chronic presentations. C. invades variety of host cell types replicates within unique vacuole derived from the lysosome. In order to understand how survives this intracellular niche, we have investigated carbon metabolism axenically cultivated bacteria. Both bacterial populations were shown assimilate exogenous [13C]glucose or [13C]glutamate, concomitant...
EMBL Australia Bioinformatics Resource (EMBL-ABR) is a developing national research infrastructure, providing bioinformatics resources and support to life science biomedical researchers in Australia. EMBL-ABR comprises 10 geographically distributed nodes with one coordinating hub, current funding provided through Bioplatforms the University of Melbourne for its initial 2-year development phase. The mission to: (1) increase Australia's capacity data sciences; (2) contribute training skills;...
The zoonotic bacterial pathogen Coxiella burnetii is the causative agent of Q fever, a febrile illness which can cause serious chronic infection. C. unique intracellular bacterium replicates within host lysosome-derived vacuoles. ability to replicate this normally hostile compartment dependent on activity Dot/Icm type 4B secretion system. In previous study, transposon mutagenesis screen suggested that disruption gene encoding novel protein CBU2072 rendered incapable replication.