A5026685311- Genetics, Bioinformatics, and Biomedical Research
- Cancer-related molecular mechanisms research
- Research Data Management Practices
- Genomics and Phylogenetic Studies
- Gene expression and cancer classification
- Scientific Computing and Data Management
- Bioinformatics and Genomic Networks
- RNA modifications and cancer
- MicroRNA in disease regulation
- RNA Research and Splicing
- Machine Learning in Healthcare
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Electronic Health Records Systems
- Artificial Intelligence in Healthcare
- Biomedical Text Mining and Ontologies
- Metabolomics and Mass Spectrometry Studies
- Genomics and Chromatin Dynamics
- Cutaneous Melanoma Detection and Management
- Artificial Intelligence in Healthcare and Education
- Advanced Text Analysis Techniques
- Machine Learning in Bioinformatics
- Gene Regulatory Network Analysis
- Melanoma and MAPK Pathways
- Aquaculture disease management and microbiota
The Alfred Hospital
2021-2025
Monash University
2017-2025
RMIT University
2023-2025
MIT University
2023-2025
Australian Genome Research Facility
2013-2021
Walter and Eliza Hall Institute of Medical Research
2015-2019
EMBL Australia
2017
The University of Melbourne
2017
Australia Bioinformatics Resource
2017
Australian Centre for Plant Functional Genomics
2016
Recent evidence has shown that the sperm epigenome is vulnerable to dynamic modifications arising from a variety of paternal environment exposures and this legacy can serve as an important determinant intergenerational inheritance. It been postulated such exchange communicated maturing spermatozoa via transfer small non-protein-coding RNAs (sRNAs) in mechanism mediated by epididymosomes; membrane bound vesicles released soma male reproductive tract (epididymis). Here we confirm mouse...
In recent years considerable effort has been devoted to understanding the epigenetic control of sperm development, leading an increased appreciation importance RNA interference pathways, and in particular miRNAs, as key regulators spermatogenesis epididymal maturation. It also shown that are endowed with impressive array miRNA have implicated various aspects fertilization embryo development. However, date there no reports on whether signature is static or it influenced by their prolonged...
Background MicroRNAs (miRNAs) are 18–23 nucleotide non-coding RNAs that regulate gene expression in a sequence specific manner. Little is known about the repertoire and function of miRNAs melanoma or melanocytic lineage. We therefore undertook comprehensive analysis miRNAome diverse range pigment cells including: melanoblasts, melanocytes, congenital nevocytes, acral, mucosal, cutaneous uveal cells. Methodology/Principal Findings sequenced 12 small RNA libraries using Illumina's Genome...
Scientific research relies on computer software, yet software is not always developed following practices that ensure its quality and sustainability. This manuscript does aim to propose new development best practices, but rather provide simple recommendations encourage the adoption of existing practices. Software promote better improves reproducibility reusability research. These are designed around Open Source values, practical suggestions contribute making source code more discoverable,...
Abstract Even in the setting of optimal resuscitation high-income countries severe sepsis and septic shock have a mortality 20–40%, with antibiotic resistance dramatically increasing this risk. To develop reference dataset enabling identification common bacterial targets for therapeutic intervention, we applied standardized genomic, transcriptomic, proteomic metabolomic technological framework to multiple clinical isolates four sepsis-causing pathogens: Escherichia coli , Klebsiella...
The functional maturation of mammalian spermatozoa is accomplished as the cells descend through highly specialized microenvironment epididymis. This dynamic environment is, in turn, created by combined secretory and absorptive activity surrounding epithelium displays an extraordinary level regionalization. Although regulatory network responsible for spatial coordination epididymal function remains unclear, recent evidence has highlighted a novel role RNA interference pathway. Indeed,...
Abstract CEPs (C-TERMINALLY ENCODED PEPTIDEs) inhibit Arabidopsis primary root growth by unknown mechanisms. We investigated how CEP3 levels control growth. peptide application decreased cell division, S-phase number, meristematic and meristem zone (MZ) size in a dose- CEP RECEPTOR1-dependent manner. Grafting showed that CEP3-dependent inhibition requires shoot CEPR1. induced mitotic quiescence MZ cells significantly faster than nutrient limitation alone. also inhibited the restoration of to...
The adoption of electronic health records (EHRs) has created opportunities to analyse historical data for predicting clinical outcomes and improving patient care. However, non-standardised representations anomalies pose major challenges the use EHRs in digital research. To address these challenges, we have developed EHR-QC, a tool comprising two modules: standardisation module preprocessing module. migrates source EHR standard format using advanced concept mapping techniques, surpassing...
Anorexia nervosa (AN) is a complex psychiatric disorder characterised by severe pathological weight loss and persistent cognitive inflexibility. Converging evidence from neuroimaging studies genome-wide association study (GWAS) suggests the involvement of prefrontal cortex (PFC) striatum dysfunction in pathophysiology AN. However, identifying causal role circuit-specific genes development AN-like phenotype remains challenging requires combination novel molecular tools preclinical models. The...
<title>Abstract</title> Healthcare data, generally available as electronic health records (EHR), provide a rich profile of an individual’s and lifestyle. This data can be harnessed for predictive modelling using machine-learning approaches. A common challenge in EHR is the prevalence missing information. Missingness occur three primary ways: completely at random (MCAR), (MAR), not (MNAR). typical approach to deal with missingness during through imputation, which could statistical or...
Esophageal adenocarcinoma (EAC) has become a major concern in Western countries due to rapid rises incidence coupled with very poor survival rates. One of the key risk factors for development this cancer is presence Barrett's esophagus (BE), which believed form response repeated gastro-esophageal reflux. In study we performed comparative, genome-wide expression profiling (using Illumina whole-genome Beadarrays) on total RNA extracted from esophageal biopsy tissues individuals EAC, BE (in...
<ns4:p>Throughout history, the life sciences have been revolutionised by technological advances; in our era this is manifested advances instrumentation for data generation, and consequently researchers now routinely handle large amounts of heterogeneous digital formats. The simultaneous transitions towards biology as a science ‘life cycle’ view research pose new challenges. Researchers face bewildering landscape management requirements, recommendations regulations, without necessarily being...
Throughout history, the life sciences have been revolutionised by technological advances; in our era this is manifested advances instrumentation for data generation, and consequently researchers now routinely handle large amounts of heterogeneous digital formats. The simultaneous transitions towards biology as a science 'life cycle' view research pose new challenges. Researchers face bewildering landscape management requirements, recommendations regulations, without necessarily being able to...
The mammalian epididymis is a highly specialized region of the male reproductive tract that lined with continuous layer epithelial cells display remarkable level regionalized secretory and absorptive activity. luminal environment created by this combined activity directly responsible for promoting functional maturation spermatozoa their maintenance in quiescent viable state prior to ejaculation. This study was designed identify complement microRNAs (miRNAs) are expressed within mouse...
What are the molecular differences between mitochondrial DNA (mtDNA)-deficient and mtDNA-normal oocytes how does supplementation alter these?Changes to methylation in a 5' cytosine-phosphate-guanine 3' (CpG) island mtDNA-specific replication factor (DNA polymerase gamma (POLG)) of mtDNA-deficient mediates an increase mtDNA copy number by 2-cell stage that positively modulates expression nuclear genes, which affect cellular metabolic processes, following autologous supplementation.Too few...
Abstract Background Diseases are complex phenotypes often arising as an emergent property of a non-linear network genetic and epigenetic interactions. To translate this resulting state into causal relationship with subset regulatory features, many experiments deploy array laboratory assays from multiple modalities. Often, each these datasets is large, heterogeneous, noisy. Thus, it non-trivial to unify interpretable phenotype. Although recent methods address problem varying degrees success,...
Despite the volume of experiments performed and data available, complex biology coronavirus SARS-COV-2 is not yet fully understood. Existing molecular profiling studies have focused on analysing functional omics a single type, which captures changes in small subset perturbations caused by virus. As logical next step, results from multiple such analysis may be aggregated to comprehensively interpret mechanisms SARS-CoV-2. An alternative approach integrate simultaneously parallel fashion...