A5019789177- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Genetics, Aging, and Longevity in Model Organisms
- Telomeres, Telomerase, and Senescence
- Plant Virus Research Studies
- Escherichia coli research studies
- Microbial Community Ecology and Physiology
- Salmonella and Campylobacter epidemiology
- Animal Virus Infections Studies
- Autophagy in Disease and Therapy
- Insect symbiosis and bacterial influences
- Viral gastroenteritis research and epidemiology
- Veterinary Oncology Research
- DNA Repair Mechanisms
- Animal Disease Management and Epidemiology
Okinawa Institute of Science and Technology Graduate University
2021-2024
The University of Melbourne
2013-2022
National Institute of Animal Health
2019
Plasma membrane damage (PMD) occurs in all cell types due to environmental perturbation and cell-autonomous activities. However, cellular outcomes of PMD remain largely unknown except for recovery or death. In this study, using budding yeast normal human fibroblasts, we found that senescence-stable cycle arrest contributing organismal aging-is the long-term outcome PMD. Our genetic screening unexpectedly identified a close association between response replicative lifespan regulations....
Mycoplasmas are simple, but successful parasites that have the smallest genome of any free-living cell and thought to a highly streamlined cellular metabolism. Here, we undertaken detailed metabolomic analysis two species, Mycoplasma bovis gallisepticum, which cause economically important diseases in cattle poultry, respectively. Untargeted gas chromatography-mass spectrometry liquid analyses mycoplasma metabolite extracts revealed significant differences steady-state levels many metabolites...
Although lipoproteins of mycoplasmas are thought to play a crucial role in interactions with their hosts, very few have had biochemical function defined. The gene encoding the lipoprotein MslA Mycoplasma gallisepticum has recently been shown be required for virulence, but this is not known. no significant sequence similarity any known function, it located within an operon M. that contains homolog previously nonspecific exonuclease. We mutagenized both genes facilitate expression Escherichia...
MalF has been shown to be required for virulence in the important avian pathogen Mycoplasma gallisepticum To characterize function of MalF, predicted part a putative ABC transporter, we compared metabolite profiles mutant with transposon inserted malF (MalF-deficient ST 04-1; ΔmalF) those wild-type bacteria using gas chromatography-mass spectrometry and liquid spectrometry. Of substrates likely transported by an transport system, glycerol was detected at significantly lower abundance ΔmalF...
Symbiomonas scintillans Guillou et Chrétiennot-Dinet, 1999 is a tiny (1.4 μm) heterotrophic microbial eukaryote. The genus was named based on the presence of endosymbiotic bacteria in its endoplasmic reticulum, however, like most such endosymbionts neither identity nor functional association with host were known. We generated both amplification-free shotgun metagenomics and whole genome amplification sequencing data from S . strains RCC257 RCC24, but unable to detect any sequences known...
The lactate dehydrogenase (LDH) of Mycoplasma genitalium has been predicted to also act as a malate (MDH), but there no experimental validation this hypothesized dual function for any mollicute. Our analysis the metabolite profile bovis using gas chromatography/mass spectrometry (GC/MS) and liquid (LC/MS) detected malate, suggesting that may be MDH activity in M. bovis. To investigate whether putative l-LDH enzyme (MDH LDH), we performed bioinformatic functional biochemical analyses....
Abstract Plasma membrane damage (PMD) occurs in all cell types due to environmental perturbation and cell-autonomous activities. However, cellular outcomes of PMD remain largely unknown except for recovery or death. Here, using budding yeast normal human fibroblasts, we show that senescence, irreversible cycle arrest contributing organismal aging, is the long-term outcome PMD. To identify genes essential response, developed a simple PMD-damaging assay detergent performed systematic...
Previously, we developed the monoclonal antibody (mAb) 449 after immunizing BALB/c mice with a live attenuated mutant of Salmonella enterica serovar Typhimurium (S. Typhimurium), and it helped protect against S. infection. Although immunogen specific for mAb449 has been found to be localized in lipopolysaccharide (LPS) region, is not clear which part LPS involved defense The present study aimed identify sites recognized by mAb449. Electron microscopy analysis showed that binds surface...