A5053962676- Alzheimer's disease research and treatments
- Prion Diseases and Protein Misfolding
- Genetics, Aging, and Longevity in Model Organisms
- Fungal and yeast genetics research
- Telomeres, Telomerase, and Senescence
- Ion channel regulation and function
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- Plant Pathogens and Fungal Diseases
- MicroRNA in disease regulation
- Cellular transport and secretion
- ATP Synthase and ATPases Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Lipid Membrane Structure and Behavior
- Biofuel production and bioconversion
- DNA Repair Mechanisms
- Endoplasmic Reticulum Stress and Disease
- Trace Elements in Health
- Genetics and Neurodevelopmental Disorders
- Electrostatics and Colloid Interactions
- Congenital heart defects research
- Erythrocyte Function and Pathophysiology
- Photoreceptor and optogenetics research
Okinawa Institute of Science and Technology Graduate University
2021-2024
Tokyo Institute of Technology
2018-2022
ORCID
2020
RIKEN Center for Brain Science
2006-2019
Big Sky Institute
2017
Abstract Given the complexity and heterogeneity of genomic architecture underlying schizophrenia, molecular analyses these patients with defined large effect-size defects could provide valuable clues. We established human-induced pluripotent stem cells from two schizophrenia 22q11.2 deletion (two cell lines each subject, total four lines) three controls (total lines). Neurosphere size, neural differentiation efficiency, neurite outgrowth, cellular migration neurogenic-to-gliogenic competence...
Plasma membrane damage (PMD) occurs in all cell types due to environmental perturbation and cell-autonomous activities. However, cellular outcomes of PMD remain largely unknown except for recovery or death. In this study, using budding yeast normal human fibroblasts, we found that senescence-stable cycle arrest contributing organismal aging-is the long-term outcome PMD. Our genetic screening unexpectedly identified a close association between response replicative lifespan regulations....
Significance On the basis of amino acid sequence, a polypeptide folds into unique structure. In contrast, aggregation-prone proteins often misfold distinct aggregate conformations. Interestingly, each conformation can dictate different phenotypic consequences, such as cellular toxicity. However, underlying mechanism origin structural diversity protein aggregates has been critical open question. Using intrinsically disordered domain yeast prion Sup35, we revealed diversification is triggered...
Protein segregation contributes to various cellular processes such as polarization, differentiation, and aging. However, the difficulty in global determination of protein hampers our understanding its mechanisms physiological roles. Here, by developing a quantitative proteomics technique, we globally monitored preexisting newly synthesized proteins during cell division budding yeast, identified crucial domains that determine cell-peripheral proteins. Remarkably, proteomic subsequent...
Endoplasmic reticulum (ER)-plasma membrane (PM) tethering is crucial for the non-vesicular lipid transport between ER and PM. However, PM-associated can impede PM binding of cytoskeletons other organelles. It poorly understood how competition cytoskeletons/organelles on resolved. Here, we show that, upon septin collar assembly, ER-PM proteins are excluded from yeast bud sites, locally detached Our results suggest that flows by polarized exocytosis extrude proteins, including sites. When...
Abstract Plasma membrane damage (PMD) occurs in all cell types due to environmental perturbation and cell-autonomous activities. However, cellular outcomes of PMD remain largely unknown except for recovery or death. Here, using budding yeast normal human fibroblasts, we show that senescence, irreversible cycle arrest contributing organismal aging, is the long-term outcome PMD. To identify genes essential response, developed a simple PMD-damaging assay detergent performed systematic...
Abstract Disaggregation of amyloid fibrils is a fundamental biological process required for propagation. However, due to the lack experimental systems, molecular mechanism how disaggregated by cellular factors remains poorly understood. Here, we established robust, in vitro reconstituted system yeast prion propagation and found that Hsp104, Ssa1, Sis1 chaperones are essential efficient disaggregation Sup35 amyloid. Real-time imaging single-molecule fluorescence coupled with reconstitution...