A5060536568- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Viral Infections and Vectors
- Respiratory viral infections research
- Plant Virus Research Studies
- Streptococcal Infections and Treatments
- Blood groups and transfusion
- Virology and Viral Diseases
- Dengue and Mosquito Control Research
- Toxoplasma gondii Research Studies
- Vector-borne infectious diseases
- Immune Cell Function and Interaction
- Animal Disease Management and Epidemiology
The University of Melbourne
2013-2024
Australian Centre for Disease Preparedness
2017
Agricultural Institute
2017
High-frequency phase and antigenic variation of homologous lipoprotein haemagglutinins has been seen in both the major avian mycoplasma pathogens, Mycoplasma synoviae gallisepticum. The expression and, hence, pMGA gene family (encoding these lipoproteins M. gallisepticum) is controlled by length a trinucleotide repeat motif 5' to promoter each gene. However, such mechanism was not detected preliminary observations on synoviae. Thus, basis for control vlhA (which encodes haemagglutinin...
Mycoplasma bovis causes a range of diseases in cattle, including mastitis, arthritis, and pneumonia. However, accurate serological diagnosis infection remains problematic. The studies described here aimed to identify an antigen that might be used develop more specific sensitive diagnostic assay. A 226-kDa immunogenic protein was consistently detected Western blots by antibodies sera from calves experimentally infected with M. bovis. This shown membrane lipase activity named mycoplasma...
ABSTRACT Mycoplasma bovis is a pathogen of emerging significance in cattle throughout the world that causing range diseases, including mastitis, arthritis, and pneumonia. The limited availability efficacy current diagnostic prophylactic tools for its control increasing antimicrobial resistance are contributing to importance beef dairy cattle. We have developed an indirect IgG enzyme-linked immunosorbent assay (ELISA) based on recombinant fragment MilA protein shown potential as effective...
ABSTRACT Mycoplasma bovis is an important emerging pathogen of cattle and bison, but our understanding the genetic basis its interactions with host limited. The aim this study was to identify genes M. required for interaction survival in association cells. One hundred transposon-induced mutants type strain PG45 were assessed their capacity survive proliferate Madin-Darby bovine kidney cell cultures. growth 19 completely abrogated, 47 had a prolonged doubling time compared parent strain. All...
The antimicrobial tylosin is commonly used to control mycoplasma infections, sometimes in combination with vaccination. However, the efficacy of a live vaccine, when combined subsequent treatment, against effects infection virulent strain unknown. This study employed differential gene expression analysis evaluate on protection provided by attenuated Vaxsafe MG ts-304 which has been shown be safe and provide long-term protective immunity Mycoplasma gallisepticum. transcriptional profiles...
Mycoplasma gallisepticum (MG) is an important poultry pathogen that causes respiratory disease and loss of production worldwide, currently controlled with live attenuated vaccines. These vaccines have limitations as they vary in their pathogenicity, the protection afforded transmissibility, but been shown to effectively reduce losses associated challenge field. A vaccine, ts-11, has used for control M. several countries. This vaccine highly dose-dependent flock antibody response weak. GapA...
There is limited understanding of the molecular basis virulence in important avian pathogen Mycoplasma gallisepticum. To define genes that may be involved colonization chickens, a collection mutants virulent Ap3AS strain M. gallisepticum were generated by signature-tagged transposon mutagenesis. The included with single insertions encoding adhesin GapA and cytadherence-related protein CrmA, Western blotting confirmed these did not express proteins. In two separate vivo screenings,...
Mycoplasma gallisepticum causes chronic respiratory disease in chickens and is also highly pathogenic turkeys. Several live attenuated M. vaccines are available for prevention of but they considered to be either not safe or efficacious The studies presented here aimed develop a suitable infection model turkeys, prerequisite development vaccine against Two wild-type Australian strains, Ap3AS 100809/31, were used their capacity induce lesions was evaluated 5-week-old 6-week-old turkeys exposed...
Live attenuated vaccines are commonly used to control Mycoplasma gallisepticum infections in chickens. M. ts-304 is a novel live vaccine strain that has been shown be safe and effective. In this study, the transcriptional profiles of genes tracheal mucosa chickens challenged with wild-type Ap3AS at 57 weeks after vaccination were explored compared unvaccinated had Ap3AS, unchallenged chickens, vaccinated but At two challenge, pair-wise comparisons transcription vaccinated-only,...
Mycoplasma gallisepticum is the primary etiological agent of chronic respiratory disease in chickens. Live attenuated vaccines are most commonly used field to control disease, but current have some limitations. Vaxsafe MG (strain ts-304) a new vaccine candidate that efficacious at lower dose than commercial strain ts-11, from which it derived. In this study, transcriptional profiles trachea unvaccinated chickens and vaccinated with ts-304 were compared 2 weeks after challenge M. Ap3AS during...
The survival, replication, and virulence of mycoplasmas depend on their ability to capture import host-derived nutrients using poorly characterized membrane proteins. Previous studies the important bovine pathogen Mycoplasma bovis demonstrated that amino-terminal end an immunogenic 226-kDa (P226) protein, encoded by milA (the full-length product which has a predicted molecular weight 303 kDa), had lipase activity. sequence MilA contains glycosaminoglycan binding motifs, as well multiple...
ABSTRACT Relatively few virulence genes have been identified in pathogenic mycoplasmas, so we used signature-tagged mutagenesis to identify mutants of the avian pathogen Mycoplasma gallisepticum with a reduced capacity persist vivo and compared levels selected experimentally infected chickens. Four had insertions one two incomplete oppABCDF operons, further three distinct hypothetical genes, containing peptidase motifs member gene family. The oppD 1 were infect chickens, all five shown...
Pathogenicity and presentation of Mycoplasma gallisepticum (MG) infection may differ from one strain to another this have implications on control measures. Infection individual birds with more than MG has been reported. A PCR followed by high resolution melt (HRM) curve analysis developed in our laboratory routinely used for detection differentiation strains. However the potential test identification strains a mixed specimen not evaluated. In present study, capability PCR-HRM technique,...
Infections caused by Mycoplasma synoviae are major welfare and economic concerns in poultry industries worldwide. These infections cause chronic respiratory disease and/or synovitis chickens turkeys leading to reduced production increased mortality rates. The live attenuated vaccine strain MS-H (Vaxsafe® MS), commonly used for protection against M. infection many countries, contains 32 single nucleotide variations compared its wildtype parent strain, 86079/7NS. Genomic analysis of strains...
Abstract Background Mycoplasma gallisepticum is a major poultry pathogen and causes severe economic loss to the industry. In mycoplasmas lipoproteins are abundant on membrane surface play critical role in interactions with host, but tools for exploring their molecular biology limited. Results this study we examined whether alkaline phosphatase gene ( phoA ) from Escherichia coli could be used as reporter mycoplasmas. The promoter region elongation factor Tu ltuf) signal acylation sequences...