A5034765313- Metabolomics and Mass Spectrometry Studies
- Advanced Chemical Sensor Technologies
- Analytical Chemistry and Chromatography
- Environmental Toxicology and Ecotoxicology
- Microbial Metabolic Engineering and Bioproduction
- Gut microbiota and health
- Parasites and Host Interactions
- Helminth infection and control
- Parasite Biology and Host Interactions
- Aquaculture Nutrition and Growth
- Water Quality and Pollution Assessment
- Probiotics and Fermented Foods
- Bacterial Identification and Susceptibility Testing
- Aluminum toxicity and tolerance in plants and animals
- Bioinformatics and Genomic Networks
- Trace Elements in Health
- Estrogen and related hormone effects
- Per- and polyfluoroalkyl substances research
- Insect symbiosis and bacterial influences
- Cardiac and Coronary Surgery Techniques
- Inflammasome and immune disorders
- Effects and risks of endocrine disrupting chemicals
- Insect-Plant Interactions and Control
- Nitric Oxide and Endothelin Effects
- Biochemical Analysis and Sensing Techniques
The University of Melbourne
2014-2021
Melbourne Genomics Health Alliance
2016-2018
Stavros Niarchos Foundation
2016
Commonwealth Scientific and Industrial Research Organisation
2014
Biotechnology Institute
2014
Umeå University
2012-2013
RMIT University
2009-2010
Australian Centre for Research on Separation Science
2009-2010
Australian Research Council
2010
The use of multiple omics techniques (i.e., genomics, transcriptomics, proteomics, and metabolomics) is becoming increasingly popular in all facets life science. Omics provide a more holistic molecular perspective studied biological systems compared to traditional approaches. However, due their inherent data differences, integrating platforms remains an ongoing challenge for many researchers. As metabolites represent the downstream products interactions between genes, transcripts, proteins,...
Anti-microbial signaling pathways are normally triggered by innate immune receptors when detecting pathogenic microbes to provide protective immunity. Here we show that the inflammasome sensor Nlrp1 aggravates DSS-induced experimental mouse colitis limiting beneficial, butyrate-producing Clostridiales in gut. The colitis-protective effects of deficiency thus reversed vancomycin treatment, but recapitulated with butyrate supplementation wild-type mice. Moreover, an activating mutation Nlrp1a...
Abstract Competition between viruses and Wolbachia for host lipids is a proposed mechanism of -mediated virus blocking in insects. Yet, the metabolomic interaction symbiont within mosquito has not been clearly defined. We compare lipid profiles Aedes aegypti mosquitoes bearing mono- or dual-infections w Mel strain dengue serotype 3 (DENV3). found metabolic signatures infection-induced intracellular events but little evidence to support direct competition lipids. Lipid dual-infected resemble...
Considering the short shelf-life of certain food products such as red meat, there is a need for rapid and cost-effective methods pathogen detection. Routine testing in laboratories mostly relies on conventional microbiological which involve use multiple selective culture media long incubation periods, often taking up to 7 days confirmed identifications. The current study investigated application omics-based approaches, proteomics using matrix-assisted laser desorption ionization...
Screening complex biological specimens such as exhaled air, tissue, blood and urine to identify biomarkers in different forms of cancer has become increasingly popular over the last decade, mainly due new instruments improved bioinformatics. However, despite some progress, identification shown be a difficult task with few (excluding recent genetic markers) being considered for introduction clinical analysis. This review describes advances gas chromatographic methods detection, diagnosis...
Due to slow diagnostics, physicians must optimize antibiotic therapies based on clinical evaluation of patients without specific information causative bacteria. We have investigated metabolomic analysis blood for the detection acute bacterial infection and early differentiation between ineffective effective treatment. A vital timely therapeutic difficulty was thereby addressed: ability rapidly detect treatment failures because antibiotic-resistant Methicillin-resistant Staphylococcus aureus...
The complex cell envelopes of Corynebacterineae contribute to the virulence pathogenic species (such as Mycobacterium tuberculosis and Corynebacterium diphtheriae) capacity nonpathogenic glutamicum) grow in diverse niches. envelope comprises an asymmetric outer membrane that overlays arabinogalactan-peptidoglycan inner membrane. Dissection lipid composition fractions is important for understanding biogenesis this multilaminate wall structure. Here, we have undertaken first high-resolution...
Mycoplasmas are simple, but successful parasites that have the smallest genome of any free-living cell and thought to a highly streamlined cellular metabolism. Here, we undertaken detailed metabolomic analysis two species, Mycoplasma bovis gallisepticum, which cause economically important diseases in cattle poultry, respectively. Untargeted gas chromatography-mass spectrometry liquid analyses mycoplasma metabolite extracts revealed significant differences steady-state levels many metabolites...
Iatrogenic hookworm therapy shows promise for treating disorders that result from a dysregulated immune system, including inflammatory bowel disease (IBD). Using murine model of trinitrobenzenesulfonic acid-induced colitis and human peripheral blood mononuclear cells, we demonstrated low-molecular-weight metabolites derived both somatic extracts (LMWM-SE) excretory-secretory products (LMWM-ESP) the hookworm, Ancylostoma caninum, display anti-inflammatory properties. Administration to mice...
Microsampling allows the collection of blood samples using a method which is inexpensive, simple and minimally-invasive, without need for specially-trained medical staff. Analysis whole provides more holistic understanding per- polyfluoroalkyl substances (PFAS) body burden. Capillary action microsamplers (Trajan hemaPEN®) allow controlled as dried spots (DBS) (four 2.74 µL ± 5 %). The quantification 75 PFAS from DBS was evaluated by comparing five common extraction techniques. Spiked (5...
Environmental pollutants such as heavy metals and fungicides pose a serious threat to waterways worldwide. Toxicological assessment of contaminants is usually conducted using single compound exposures, it challenging understand the effect mixtures on biota standard ecotoxicological methods; whereas complex chemical are more probable in ecosystems. This study exposed Simplisetiaaequisetis (an estuarine annelid) sublethal concentrations metal (zinc) fungicide (boscalid), both singly mixture,...