A5005991775- Antibiotic Resistance in Bacteria
- Pharmaceutical and Antibiotic Environmental Impacts
- Enterobacteriaceae and Cronobacter Research
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Bacterial Identification and Susceptibility Testing
- Infections and bacterial resistance
- Antibiotics Pharmacokinetics and Efficacy
- Aquaculture disease management and microbiota
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Mycobacterium research and diagnosis
- Antibiotic Use and Resistance
- Plant Pathogenic Bacteria Studies
- Antimicrobial Resistance in Staphylococcus
- Probiotics and Fermented Foods
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Microbial infections and disease research
- Yersinia bacterium, plague, ectoparasites research
- Gut microbiota and health
- Microbial Metabolites in Food Biotechnology
- Myxozoan Parasites in Aquatic Species
- Insect and Pesticide Research
Charles University
2020-2025
University Hospital Brno
2021-2025
University of Veterinary Science
2025
Mendel University in Brno
2025
University of Veterinary Sciences Brno
2015-2024
Central European Institute of Technology
2015-2024
University of Technology Sydney
2023
Park University
2022
Istituto Superiore di Sanità
2012-2013
Aims: To carry out an assessment of the occurrence resistance to antimicrobials in Escherichia coli that has been isolated from young Black‐headed Gulls three nesting colonies. Methods and Results: A total 257 isolates were tested for sensitivity eight antibacterial substances by disk diffusion method. The polymerase chain reaction was used detecting specific genes class 1 integrons resistant E. isolates. 75 (29·9%) one or more antimicrobial agents. dominant type tetracycline, detected 49...
Individual cloacal swabs of mallards (Anas platyrhynchos) and herring gulls (Larus argentatus), as well samples waterbird feces obtained in 2008 2009, were cultivated for Escherichia coli. Isolates E. coli tested susceptibilities to 12 antimicrobial agents by the disk diffusion method. Moreover, subcultivated on MacConkey agar (MCA) containing cefotaxime (2 mg liter(-1)) detect with extended-spectrum beta-lactamase (ESBL) subsequently MCA supplemented ciprofloxacin (0.05 nalidixic acid (20...
To determine the presence of antibiotic-resistant faecal Escherichia coli in populations wild mammals Czech Republic and Slovakia.Rectal swabs or faeces collected during 2006-2008 from were spread on MacConkey agar containing 2 mg l(-1) cefotaxime. From plates with positive growth, one isolate was recovered identified as E. coli. Susceptibility to 12 antibiotics tested using disk diffusion method. Resistance genes, class 1 integrons gene cassettes detected resistant isolates by polymerase...
To characterize the pNDM-CIT plasmid identified in Citrobacter freundii carrying genes encoding metallo-β-lactamase NDM-1 and 16S RNA methylase ArmA. The complete DNA sequence of was obtained by using 454-Genome Sequencer FLX procedure on a library purified from Escherichia coli J53 transconjugant. Contig assembly predicted gaps were confirmed filled PCR-based gap closure. Comparative analysis with IncHI1 incompatibility group plasmids performed BLASTN BLASTP algorithms. Plasmid 288 920 bp...
Wild animals foraging in the human-influenced environment are colonized by bacteria with clinically important antibiotic resistance. The occurrence of such wildlife is influenced various biological, ecological, and geographical factors which have not yet been fully understood.
The objective of this study was to investigate the silver gull as an indicator environmental contamination by salmonellae and carbapenemase-producing Enterobacteriaceae (CPE) in south-east Australia.A total 504 cloacal samples were collected from chicks at three nesting colonies New South Wales, Australia [White Bay (n = 144), Five Islands 200) Montague Island 160)] examined for CPE. Isolates tested carbapenemase genes susceptibility 14 antibiotics. Clonality determined PFGE MLST. Genetic...
The global occurrence of antibiotic resistance genes in bacteria water environments is an increasing concern. Treated wastewater was sampled daily over a 45 day period from the outflow municipal treatment plant Brno, Czech Republic, and examined for extended-spectrum β-lactamase (ESBL)-producing bacteria. Water samples were cultivated on MacConkey agar with cefotaxime (2 mg/L) individual colonies ESBL production. Phenotypic ESBL-positive identified as Escherichia coli or Klebsiella spp....
The pathogenic extended-spectrum-beta-lactamase (ESBL)-producing Escherichia coli lineage ST648 is increasingly reported from multiple origins. Our study of a large and global collection various hosts (87 whole-genome sequences) combining core accessory genomics with functional analyses in vivo experiments suggests that nascent generalist lineage, lacking clear phylogeographic host association signals.
Abstract Escherichia coli ST58 has recently emerged as a globally disseminated uropathogen that often progresses to sepsis. Unlike most pandemic extra-intestinal pathogenic E. (ExPEC), which belong phylogroup B2, belongs the environmental/commensal B1. Here, we present pan-genomic analysis of global collection 752 isolates from diverse sources. We identify large sub-lineage characterized by near ubiquitous carriage ColV plasmids, carry genes encoding virulence factors, and distinct accessory...
Introduction Hospitals and wastewater are recognized hot spots for the selection dissemination of antibiotic-resistant bacteria to environment, but total participation hospitals in spread nosocomial pathogens municipal treatment plants (WWTPs) adjacent rivers had not previously been revealed. Methods We used a combination culturing whole-genome sequencing explore transmission routes Escherichia coli from hospitalized patients suffering urinary tract infections (UTI) via environment. Samples...
To examine surface water from a pond in the northeastern part of Czech Republic and young black-headed gulls (Larus ridibundus) nesting on same for presence antibiotic-resistant Salmonella Escherichia coli.A total 16% (n = 87) 24% 216) gull samples yielded Salmonella. Enteritidis PT8 PT4 were most prevalent. Antibiotic resistance was found 12% 14) 28% 51) salmonellae. coli 83 (95%) 213 (99%) samples, respectively. Totals 18% 83) 213) E. isolates resistant to antimicrobial agents tested....
The aim of this study was to determine the occurrence extended-spectrum β-lactamase (ESBL)-producing Escherichia coli at an equine clinic and a horseback riding centre, discuss impact antimicrobial treatment on resistance selection. Faeces from horses, environmental smears flies were sampled both centre. Staff also examined. samples cultivated MacConkey agar with cefotaxime (2 mg/L) isolate ESBL-producing E. coli. presence bla qnr genes tested by PCR, transferability determined conjugation....
Aims: To determine the presence of antibiotic-resistant faecal Escherichia coli and Enterococcus spp. in feral pigeons (Columba livia forma domestica) Czech Republic. Methods Results: Cloacal swabs collected city Brno 2006 were cultivated for E. coli. Resistance genes, class 1 2 integrons, gene cassettes detected resistant isolates by polymerase chain reaction (PCR). The samples also enterococci. Species status enterococci was determined using repetitive extragenic palindromic-PCR. genes...
The aim of the study was to characterize a collection Escherichia coli and Salmonella harbouring qnr bla(CTX-M-1) genes on IncN plasmids isolated from humans, food-producing, companion wild animals, environment six European countries.Nineteen were compared using restriction fragment length polymorphism (RFLP), plasmid multilocus sequence typing (pMLST) hybridization with repN, qnrS1, qnrB19 or probes. Plasmids pKT58A pHHA45 sequenced 454-Genome Sequencer FLX platform library constructed DNA...
Escherichia coli is a common commensal bacterial species of humans and animals that may become troublesome pathogen causing serious diseases. The aim this study was to characterize the quinolone resistance phenotypes genotypes in E. isolates different origin from one area Czech Republic. were obtained hospitalized patients outpatients, chicken farms, retailed turkeys, rooks wintering wastewaters. Susceptibility grown on MacConkey agar with ciprofloxacin (0.05 mg/L) 23 antimicrobial agents...
To describe the prevalence and diversity of IncX plasmids with antibiotic resistance genes in Enterobacteriaceae to identify most disseminated lineages plasmid family.IncX were screened 1894 isolates resistant cefotaxime (2 mg/L) or reduced susceptibility ciprofloxacin (0.05 obtained from various sources five continents using PCR. plasmid-harbouring identified MALDI-TOF biochemical tests, for PCR sequencing; their clonality was determined by PFGE. Horizontal transfer tested transformation...
By compiling various genomic and phenotypic data sets, we have provided one of the most comprehensive studies Escherichia coli isolates from Australian silver gull, on media containing clinically relevant antibiotics. The analysis genetic structures capturing antimicrobial resistance genes across three gull breeding colonies in New South Wales, Australia, comparisons to clinical revealed a range trackable signatures that highlight broad distribution more than 170 different lineages E. .
Extended-spectrum-beta-lactamase (ESBL)-producing, AmpC beta-lactamase-producing, and plasmid-mediated quinolone resistance (PMQR) gene-positive strains of Escherichia coli were investigated in wintering rooks (Corvus frugilegus) from eight European countries. Fecal samples (n = 1,073) the Czech Republic, France, Germany, Italy, Poland, Serbia, Spain, Switzerland examined. Resistant isolates obtained selective cultivation screened for ESBL, AmpC, PMQR genes by PCR sequencing. Pulsed-field...
Faecal Escherichia coli strains were isolated from great cormorants (Phalacrocorax carbo) and mallards (Anas platyrhynchos), which are commonly occurring waterbirds in Europe, studied for resistance to cephalosporins fluoroquinolones. Cloacal swabs or faeces Central Europe cultivated isolate with extended-spectrum β-lactamase (ESBL) plasmid-mediated quinolone (PMQR) genes. Ten ESBL-producing E. the blaCTX-M-15 blaCTX-M-27 gene eight (1.6%, n = 499). The blaCTX-M genes harboured by plasmids...
Silver gulls carry phylogenetically diverse Escherichia coli, including globally dominant extraintestinal pathogenic E. coli (ExPEC) sequence types and pandemic ExPEC-ST131 clades; however, our large-scale study (504 samples) on silver nesting off the coast of New South Wales identified ST457 as most prevalent. A phylogenetic analysis whole-genome sequences (WGS) 138 samples comprising 42 from gulls, 2 humans (Australia), 14 poultry farmed in Paraguay were compared with 80 WGS deposited...
Escherichia coli ST131 is a globally dispersed extraintestinal pathogenic E. lineage contributing significantly to hospital and community acquired urinary tract bloodstream infections. Here we describe detailed phylogenetic analysis of the whole genome sequences 284 Australian isolates from diverse sources, including clinical, food companion animals, wildlife environment. Our phylogeny results single nucleotide polymorphism (SNP) show typical clade distribution with clades A, B C clearly...