A5069031949- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Bacteriophages and microbial interactions
- Infections and bacterial resistance
- Gut microbiota and health
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacillus and Francisella bacterial research
- Escherichia coli research studies
- Microbial infections and disease research
- Bacterial biofilms and quorum sensing
- Antimicrobial Resistance in Staphylococcus
- Genomics and Phylogenetic Studies
- Vibrio bacteria research studies
- Streptococcal Infections and Treatments
- Mycobacterium research and diagnosis
- Advanced Proteomics Techniques and Applications
- Clostridium difficile and Clostridium perfringens research
- Aquaculture disease management and microbiota
- Ginseng Biological Effects and Applications
- Microbial Community Ecology and Physiology
- Bacterial Infections and Vaccines
- Pneumonia and Respiratory Infections
- Bacterial Identification and Susceptibility Testing
- Animal Nutrition and Physiology
- Animal Behavior and Welfare Studies
University of Warwick
2023-2024
Central European Institute of Technology
2019-2024
University of Glasgow
2021-2024
University of Hradec Králové
2022-2023
Veterinary Research Institute
2016-2021
University of Veterinary Sciences Brno
2019-2021
Charles University
2019-2021
Masaryk University
2017-2019
In order to start understand the function of individual members gut microbiota, we cultured, sequenced and analysed bacterial anaerobes from chicken caecum.Altogether 204 isolates caecum were obtained in pure cultures using Wilkins-Chalgren anaerobe agar anaerobic growth conditions. Genomes all determined NextSeq platform subjected bioinformatic analysis. Among identified 133 different strains belonging seven phyla - Firmicutes, Bacteroidetes, Actinobacteria, Proteobacteria, Verrucomicrobia,...
The aim of this study was to characterize the first cases and outbreaks OXA-48-like-producing Enterobacteriaceae recovered from hospital settings in Czech Republic. From 2013 2015, 22 Klebsiella pneumoniae isolates, 3 Escherichia coli 1 Enterobacter cloacae isolate producing OXA-48-like carbapenemases were isolated 20 patients. Four patients colonized or infected by two three different producers. K. isolates classified into nine sequence types (STs), with ST101 being predominant (n = 8). E....
The aim of the present study was to characterize sporadic cases and an outbreak NDM-like-producing Enterobacteriaceae recovered from hospital settings, in Czechia. During 2016, 18 Entrobacteriaceae isolates including 10 Enterobacter cloacae complex (9 E. xiangfangensis 1 asburiae), 4 Escherichia coli, Kluyvera intermedia, Klebsiella pneumoniae, oxytoca, Raoultella ornithinolytica that produced NDM-like carbapenemases were isolated 15 patients. Three patients colonized or infected by two...
Carbapenem-resistant Acinetobacter baumannii (CRAB) has been implicated in hospital outbreaks worldwide. Here, we present a whole-genome-based investigation of an extensively drug-resistant CRAB outbreak rapidly spreading and causing high incidences mortality at numerous wards large tertiary Lebanon. This is the first study its kind region. Two circulating clones were identified using combination molecular typing approaches, short- long-read sequencing Bayesian transmission network analysis....
Different housing systems can be used in pig production and little is known about their effect on gut microbiota composition. In this study we characterized fecal by sequencing the rRNA genes sows kept during gestation conventional pens with a slatted floor enriched covered deep straw. After farrowing, of 1- 4-day-old piglets were also monitored. Microbiota from system contained significantly more Prevotella, Parabacteroides, CF231, Phascolarctobacterium, Fibrobacter, Anaerovibrio YRC22 less...
ABSTRACT The objective of this study was to perform molecular surveillance for assessing the spread carbapenemase-producing Pseudomonas aeruginosa in Czech hospitals. One hundred thirty-six isolates were recovered from 22 hospitals located throughout country. Sequence type 357 (ST357) dominated ( n = 120) among carbapenemase producers. seventeen produced IMP-type (IMP-7 [ 116] and IMP-1 1]) metallo-β-lactamases (MβLs), 15 VIM-2 MβL, remaining expressed GES-5 enzyme. bla IMP -like genes three...
The occurrence of colistin resistance has increased rapidly among Enterobacterales around the world. We performed a national survey plasmid-mediated in human clinical isolates through retrospective analysis samples from 2009 to 2017 and prospective sampling 2018–2020. aim this study was identify characterize with mcr genes various regions Czech Republic using whole genome sequencing (WGS). Of all 1932 colistin-resistant analyzed, 73 (3.8%) were positive for genes. Most carried mcr-1 (48/73)...
In this study we determined protein and gene expression in the caeca of newly hatched chickens inoculated with cecal contents sourced from hens different ages. Over 250 proteins exhibited modified levels response to microbiota inoculation. The most significant inductions were observed for ISG12-2, OASL, ES1, LYG2, DMBT1-L, CDD, ANGPTL6, B2M, CUZD1, IgM Ig lambda chain. Of these, ES1 both immunoglobulins expressed at lower germ-free compared conventional chickens. contrast, CELA2A, BRT-2,...
Here, we describe two plasmids carrying mcr-4.3 in Acinetobacter baumannii strains isolated from imported food and a clinical sample. The comparative analysis of these plasmids, with other reported the NCBI database, highlighted common origin plasmidic structure This is first case gene A. strain Europe. We hypothesize that import initiating spread Czech Republic.
Infectious diseases threaten endangered species, particularly in small isolated populations. Seabird populations on the remote Amsterdam Island Indian Ocean have been decline for past three decades, with avian cholera caused by Pasteurella multocida proposed as primary driver. However, Erysipelothrix species also sporadically detected from albatrosses and may be contributing to some of observed mortality. In this study, we genomically characterized 16 isolates obtained yellow-nosed albatross...
A total of 18 Enterobacteriaceae (17 from gulls and 1 a clinical sample) collected Australia, carrying IncA/C plasmids with the IMP-encoding In809-like integrons, were studied. Seven plasmids, being representatives different origins, plasmid sizes, replicon combinations, resistance genes, completely sequenced. Plasmid pEc158, identified in Escherichia coli ST752 isolate, showed extensive similarity to type 2 IncA/C2 plasmids. pEc158 carried none blaCMY-2-like region or ARI-B ARI-A regions,...
Conjugative plasmids from the IncHI1 incompatibility group play an important role in transferring antibiotic resistance Salmonella Typhimurium. However, knowledge of their genome structure or gene expression is limited. In this study, we determined complete nucleotide sequences four to antibiotics by two different next generation sequencing protocols and protein mass spectrometry. Sequence data including additional 11 GenBank were used for definition plasmid core-genome pan-genome. The...
The aim of our study was to determine complete nucleotide sequence mcr-1- carrying plasmids from Enterobacterales isolates recovered domestic and imported raw retailed meat compare them with available at the GenBank database. A set 16 originating Escherichia coli ( n = 13), Klebsiella pneumoniae 2), Citrobacter braakii 1) were analyzed. In previous study, data whole genome sequencing showed that mcr-1 gene located on different incompatibility groups (IncHI2, IncI2, IncX4). IncI2 3) IncX4 8)...
The complete nucleotide sequences of six IMP-4-encoding plasmids recovered from Enterobacteriaceae isolates wildlife origin were characterized. Sequencing data showed that different incompatibility groups (IncM, IncI1, IncF, and nontypeable [including an IncX5_2 two pPrY2001-like]) carried the blaIMP-4-carrying integrons In809 or In1460. Most mph(A) region, chrA-like, aac(3)-IId, blaTEM-1b genes. Finally, plasmid analysis revealed involvement IS26- Tn1696-associated mechanisms in...
The aim of the present study was to determine rate and mechanisms resistance macrolides, lincosamides, streptogramin B (MLSB) antibiotics Staphylococcus aureus collected in Central Greece. Of 2,893 S. during 2012-2017, 1,161 isolates (40.2%) exhibited at least one MLSB agents. erythromycin statistically significantly higher methicillin-resistant (MRSA) (58.6%) than methicillin-sensitive (MSSA) (20.7%) (p = 0.002). Two hundred seventy-five representative MLSB-resistant aureus, including 81...
Escherichia coli sequence type 963 (ST963) is a neglected lineage closely related to ST38, globally widespread extraintestinal pathogenic ST causing urinary tract infections (UTI) as well sepsis in humans. Our current study aimed improve the knowledge of this understudied by carrying out comprehensive comparative analysis whole-genome sequencing data consisting 31 isolates from silver gulls Australia along with another 80 genomes public resources originating geographically scattered regions....
The study aimed to monitor the fecal shedding of cefotaxime-resistant Escherichia coli (CREC) in a cohort healthy calves on dairy farm with documented antimicrobial usage and characterize selected AmpC beta-lactamase-producing E. isolates. Fecal samples from 13 suckling (1–63 d age; 113 total) were repeatedly collected cultivated MacConkey agar cefotaxime (2 mg/L). Resistant colonies counted, one colony obtained highest dilution each sample was identified by matrix-assisted laser...
Candida albicans is an opportunistic pathogen accounting for the majority of cases infections. Currently, C. are developing resistance towards different classes antifungal drugs and this has become a global health burden that does not spare Lebanon. This study aims at determining point mutations in genes known to be involved acquisition correlating virulence ergosterol content azole resistant isolate CA77 from pilot first its kind implemented We carried out whole genome sequencing examined...
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