A5070927734- Cholinesterase and Neurodegenerative Diseases
- Mitochondrial Function and Pathology
- Spectroscopy Techniques in Biomedical and Chemical Research
- Fatty Acid Research and Health
- Heavy Metal Exposure and Toxicity
- Insect and Pesticide Research
- transportation and logistics systems
- Computational Drug Discovery Methods
- Bone Tissue Engineering Materials
- Transport and Logistics Innovations
- Nanoparticles: synthesis and applications
- Advanced Fluorescence Microscopy Techniques
- Nuclear Structure and Function
- Chemotherapy-induced organ toxicity mitigation
- Selenium in Biological Systems
- Mycotoxins in Agriculture and Food
- TiO2 Photocatalysis and Solar Cells
- Drug-Induced Hepatotoxicity and Protection
- Sphingolipid Metabolism and Signaling
- Natural Products and Biological Research
- ATP Synthase and ATPases Research
- Carbohydrate Chemistry and Synthesis
- Titanium Alloys Microstructure and Properties
- bioluminescence and chemiluminescence research
- Forest Insect Ecology and Management
University of Pardubice
2017-2025
Herrenknecht (Germany)
1977
This work aims to investigate the chemical and/or structural modification of Ti and Ti-6Al-4V (TiAlV) alloy surfaces possess even more favorable properties toward cell growth. These modifications were achieved by (i) growing TiO
The present work exploits Ti sheets and TiO2 nanotube (TNT) layers their surface modifications for the proliferation of different cells. with a native oxide layer, crystalline thermal two kinds TNT (prepared via electrochemical anodization) defined inner diameter 12 15 nm were used as substrates. A part was additionally coated by thin coatings using atomic layer deposition (ALD). An increase in cell growth WI-38 fibroblasts (>50%), MG-63 osteoblasts (>30%), SH-SY5Y neuroblasts (>30%)...
Alzheimer’s disease (AD) is a complex neurodegenerative disorder with an unclear etiology. Current treatments, primarily cholinesterase (ChE) inhibitors and N-methyl-D-aspartate receptor (NMDAR) antagonists, offer only symptomatic relief. Drugs targeting one pathological condition have generated limited efficacy. Thus, combining two or more therapeutic interventions into molecule believed to provide higher benefits for the treatment of AD. In this study, we designed, synthesized,...
Titanium dioxide nanoparticles, 25 nm in size of crystallites (TiO2 P25), are among the most produced nanomaterials worldwide. The broad use TiO2 P25 material science has implied a request to evaluate their biological effects, especially lungs. Hence, pulmonary A549 cell line been used estimate effects P25. However, reports have provided dissimilar results on caused toxicity. Surprisingly, physicochemical factors influencing action models not evaluated reports. Thus, objective present study...
Abstract At present, nuclear condensation and fragmentation have been estimated also using Hoechst probes in fluorescence microscopy flow cytometry. However, none of the methods used for quantitative spectrofluorometric assessment. Therefore, aim present study was to develop a assay detection intact cells. We human hepatoma HepG2 renal HK-2 cells cultured 96-well plates treated with potent apoptotic inducers (i.e. cisplatin, staurosporine, camptothecin) 6–48 h. Afterwards, were incubated...
Oxime cholinesterase reactivators (oximes) are used to counteract organophosphate intoxication. Charged oximes administered via intramuscular or intravenous injection when the majority of dose is unmetabolized and excreted as urine. In this study, effects selected double charged were determined in HK-2 cell line a model for renal toxicity screening. Some on dehydrogenase activity found obidoxime, asoxime (syn. HI-6), K027, K203. The K868 K869 be unreliable due rapid degradation both...
Cadmium is a heavy metal causing toxicity especially in kidney cells. The linked also with enhanced oxidative stress leading to cell death. On the other hand, our recent experiments have shown that an increase of total intracellular dehydrogenases activity can occur cells before declining until aim present study, therefore, was evaluate this transient enhancement viability after cadmium treatment. human HK-2 line treated CdCl2 at concentrations 0-200 µM for 2-24 h and dehydrogenase tested....
The simple preparation of the natural sphingoid bases possessing cytotoxic activity – marine drugs Clavaminol A and Xestoaminol C all their unnatural stereoisomers in high enantiomeric purity (ca. 95 % major enantiomer) was described. individual enantiomers were obtained by utilization asymmetric Henry reaction. diastereomers target compounds separated column chromatography after transformation into corresponding 2‐phenyloxazoline derivatives. evaluated for antiproliferative cancer cell...
The human proximal tubular HK-2 cell line is an immortalized commonly used for studying toxicity. Even as their use presently increasing, there unfortunately are no studies focused on functional changes in cells associated with passaging. aim of the present study, therefore, was to evaluate stability during 13 weeks continuous passaging after 6 and 24 h treatment model nephrotoxic compounds (i.e., acetaminophen, cisplatin, CdCl(2)). Short tandem repeat profile, doubling time, diameter,...
Alzheimer’s disease (AD) is a complex with an unknown etiology. Available treatments, limited to cholinesterase inhibitors and N-methyl-d-aspartate receptor (NMDAR) antagonists, provide symptomatic relief only. As single-target therapies have not proven effective, rational specific-targeted combination into single molecule represents more promising approach for treating AD, expected yield greater benefits in alleviating symptoms slowing progression. In the present study, we designed,...
Abstract Acetaminophen (APAP) belong among the most used analgesics and antipyretics. It is structurally derived from p -aminophenol (PAP), a potent inducer of kidney toxicity. Both compounds can be metabolized to oxidation products conjugated with glutathione. The glutathione-conjugates cleaved provide cysteine conjugates considered as generally nontoxic. aim present report was synthesize purify both APAP- PAP-cysteine and, first study at all, evaluate their biological effects in human HK-2...
Abstract At present, nuclear condensation and fragmentation have been estimated also using Hoechst probes in fluorescence microscopy flow cytometry. However, none of the methods has used for quantitative spectrofluorometric assessment. Therefore, aim present study was to develop a assay detection intact cells. We HepG2 HK‑2 cells cultured 96-well plates which were treated with potent apoptotic inducers (i.e. cisplatin, staurosporine, camptothecin) 6-48 h. Then, incubated 33258 (2 µg/mL)...
Abstract Labelling of bark beetles (Col. Scolytidae) by means indicator activation Different tracers and tagging methods have been investigated to label analysis. In large series laboratory experiments, the most homogeneous distribution highest labelling stability was achieved submerging for several hours in a precooled solution Lanthanum chloride (LaCl3). Zusammenfassung 1 Mit den beschriebenen Untersuchungen wurde eine Methode zur Markierung größerer Borkenkäfermengen erarbeitet. Das...