Research background. Cascara, the dried husk of coffee cherries, has gained attention as a potential beverage due to its unique flavor profile and health benefits. Traditionally, cascara been prepared using hot brewing methods. However, recent interest in cold techniques led investigations into how temperature affects functional properties beverages. Experimental approach. Colour (CIEL*a*b*), total dissolved solids, titratable acidity were determined beverages at 5, 10, 15 20 °C. Phenolics...

Abstract A comprehensive 2‐D LC×LC system was developed for the separation of phenolic and flavone antioxidants, using a PEG‐silica column in first dimension C 18 with porous‐shell particles or monolithic second dimension. Combination PEG C18 C8 stationary phase chemistries provide low selectivity correlations between systems. This evidenced by large differences structural contributions to retention ‐COOH, ‐OH other substituents on basic phenol structure. Superficially porous columns fused...

Abstract The goal of this work is the study possibilities two basic separation modes used in analysis complex triacylglycerol (TG) samples plant oils and animal fats, i.e. non‐aqueous reversed‐phase (NARP) silver‐ion HPLC coupled with atmospheric pressure chemical ionization mass spectrometry (APCI‐MS). orthogonality both tested for TG mixtures containing fatty acids (FAs) different acyl chain lengths, number, positions geometry double bonds (DBs) regioisomeric FAs on glycerol skeleton....

Gradient elution provides significant improvement in peak capacity with respect to isocratic conditions. In the second dimension, gradients are limited a short-time period available for separation. Various types of second-dimension comprehensive LC x compared: (i) "full fraction", (ii) "segment fraction" and (iii) "continuously shifting" gradients, applied orthogonal separations phenolic acids flavones on polyethylene glycol column first dimension two porous shell fused-core C18 columns...

Balsamic vinegar is one of the best known and most popular types vinegar, it a rich source polyphenolic compounds. The quality balsamic as well content phenolic substances vary depending on production method. In present work, we have developed method for comprehensive characterization compounds in vinegars based combination gas chromatography (GC) high-performance liquid (HPLC) coupled with mass spectrometric detection single mode (MS) tandem (MS/MS). total, 14 samples different were...

The applicability and predictive properties of the linear solvent strength model two nonlinear retention‐time models, i.e., quadratic Neue model, were assessed for separation small molecules (phenol derivatives), peptides, intact proteins. Retention‐time measurements conducted in isocratic mode gradient applying different times elution‐strength combinations. provided most accurate retention‐factor predictions (average absolute prediction error 1.5%) peptides separations (with a 2.3%). An...

Abstract The aim of the work is to contribute rational selection columns and mobile phases for ‘orthogonal’ two‐dimensional LC × operation specific sample types, providing maximum increase in peak capacity with respect single‐dimension HPLC. As a criterion suitability, dissimilarity separation selectivity phase systems was selected. For this purpose, correlations between retention molecular structure descriptors were used characterize compare various types selective interactions using...

A family of new compounds with sulfonamide and amide functional groups as potential Alzheimer's disease drugs were prepared by multistep synthesis. Thermal stability measurements recorded the initial decomposition in range 200-220°C, close above melting point. The final tested for their ability to inhibit acetylcholinesterase butyrylcholinesterase, vitro dissolution behavior selected was studied through both lipophilic hydrophilic matrix tablets. All nine derivatives even more active...

Abstract A new capillary electrophoretic method for the chiral separation of four major Cinchona alkaloids (quinine/quinidine and cinchonine/cinchonidine) was developed using heptakis‐(2,6‐di‐ O ‐methyl)‐β‐cyclodextrin as selector. The inner walls were modified with a thin polyacrylamide layer, which substantially reduced electroosmotic flow improved resolution reproducibility migration time analytes. Various operation parameters optimised, including pH, temperature, concentration background...

Abstract A 2‐D method was developed for separation of phenolic acids and flavone compounds combining LC with MEKC. The effect substituted neutral anionic CD additives to the background electrolyte on quality MEKC investigated. best selectivity achieved in 25 mmol/L borate buffer at pH 9.05 addition 10 g/L SDS 1.85 heptakis (6‐ O ‐sulfo)‐β‐CD. These conditions were used second dimension combination a PEG column first dimension, providing orthogonality (the lowest degree correlation between...

Acetaminophen (APAP) overdose causes liver injury, but in some cases it is associated also with renal impairment. While several studies exist relation to acetaminophen nephrotoxicity, no reports have been published describing intracellular changes related APAP nephrotoxicity vitro. Because proximal tubular cells are considered constitute a secondary site of drug-induced injury after hepatocytes, our study's aim was estimate the toxicity human HK-2 cell line. We used range concentrations...

Vinegars are natural products manufactured by two-step fermentation. One of the most famous is balsamic vinegar, especially that produced in Modena, Italy. Its unique production process positively distinguishes it from other vinegars. There basically three types vinegar: common Balsamic vinegar and Traditional Modena. The chemical analysis these vinegars mainly carried out using gas or liquid chromatography, often coupled to mass spectrometric detection. Although chromatography generally...

A 2 D method was developed for separation of phenolic acids and flavonoids natural antioxidants combining LC with MEKC . The in‐capillary preconcentration step applied the improvement sensitivity before second dimension analysis. influence first mobile phase composition on migration times in evaluated. When gradient elution is ‐ system, increasing concentration organic solvent fractions transferred from influences electroosmotic flow, partitioning equilibria samples micelles properties...