A5004564802- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Protein purification and stability
- Innovative Microfluidic and Catalytic Techniques Innovation
- Chromatography in Natural Products
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- SARS-CoV-2 and COVID-19 Research
- Microfluidic and Bio-sensing Technologies
- Bacteriophages and microbial interactions
- Crystallization and Solubility Studies
- 3D Printing in Biomedical Research
- SARS-CoV-2 detection and testing
- Nanopore and Nanochannel Transport Studies
- Glycosylation and Glycoproteins Research
- Pesticide Residue Analysis and Safety
- Electrostatics and Colloid Interactions
- Nanofabrication and Lithography Techniques
- T-cell and Retrovirus Studies
- Advanced Chemical Sensor Technologies
- Transgenic Plants and Applications
- Cytomegalovirus and herpesvirus research
- interferon and immune responses
- Smart Materials for Construction
- Chemical and Physical Properties in Aqueous Solutions
Vrije Universiteit Brussel
2014-2024
Research Institute for Chromatography
2024
BOKU University
2021
Faculty (United Kingdom)
2020
Engineering (Italy)
2020
University of Groningen
2015
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTAdvances in Ultrahigh-Pressure Liquid Chromatography Technology and System DesignJelle De Vos, Ken Broeckhoven, Sebastiaan Eeltink*View Author Information Vrije Universiteit Brussel, Department of Chemical Engineering, Pleinlaan 2, B-1050, Brussels, Belgium*Phone: +32 (0)2 629 3324. Fax: 3248. E-mail: [email protected]Cite this: Anal. Chem. 2016, 88, 1, 262–278Publication Date (Web):November 24, 2015Publication History Published online8 December...
Elimination of the binding immunoglobulin Fc to gamma receptors is highly desirable for avoidance unwanted inflammatory responses therapeutic antibodies and fusion proteins. Many different approaches have been used in clinic, but they not systematically compared. We now produced a matched set anti-CD20 with subclasses variants compared their activity C1q, Fc-gamma cell-based assays. Most still significant levels one or more these assays many them impaired temperature stability corresponding...
Antibody tests are essential tools to investigate humoral immunity following SARS-CoV-2 infection or vaccination. While first-generation antibody have primarily provided qualitative results, accurate seroprevalence studies and tracking of levels over time require highly specific, sensitive quantitative test setups.
This study presents a comprehensive investigation of the mechanistic understanding retention and selectivity in hydrophobic interaction chromatography. It provides valuable insights into crucial method-development parameters involved achieving chromatographic resolution for profiling molecular variants trastuzumab. Retention characteristics have been assessed three column chemistries, i.e., butyl, alkylamide, long-stranded multialkylamide ligands, while distinguishing hydrophobicity surface...
This study discusses the design aspects for construction of a microfluidic device comprehensive spatial two‐dimensional liquid chromatography. In chromatography each peak is characterized by its coordinates in plane. After completing first‐dimension separation all fractions are analyzed parallel second‐dimension separations. Hence, potentially provides much higher peak‐production rates than coupled column multi‐dimensional approach which analyses performed sequentially. A chip has been...
The applicability and predictive properties of the linear solvent strength model two nonlinear retention‐time models, i.e., quadratic Neue model, were assessed for separation small molecules (phenol derivatives), peptides, intact proteins. Retention‐time measurements conducted in isocratic mode gradient applying different times elution‐strength combinations. provided most accurate retention‐factor predictions (average absolute prediction error 1.5%) peptides separations (with a 2.3%). An...
The selectivity and separation efficiency of aqueous size-exclusion chromatographic separations intact proteins were assessed for different flow rates, using columns packed with 3 5 μm silica particles containing 150 290 Å stagnant pores. A mixture molecular weights ranging between 17 000 670 Da was used to construct the calibration curves. Both model fit predictive properties, a leave-one-out strategy, polynomial models (up fifth order) evaluated rates. best compromise properties obtained...