A5058896515- Viral Infectious Diseases and Gene Expression in Insects
- Transgenic Plants and Applications
- Virus-based gene therapy research
- Monoclonal and Polyclonal Antibodies Research
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Probiotics and Fermented Foods
- Protein purification and stability
- Poxvirus research and outbreaks
- Herpesvirus Infections and Treatments
- Insect Resistance and Genetics
- CRISPR and Genetic Engineering
- Glycosylation and Glycoproteins Research
- Enzyme Production and Characterization
- Influenza Virus Research Studies
- RNA and protein synthesis mechanisms
- Invertebrate Immune Response Mechanisms
- RNA Interference and Gene Delivery
- Microbial Metabolites in Food Biotechnology
- Genomics and Phylogenetic Studies
- Advanced Biosensing Techniques and Applications
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Viral gastroenteritis research and epidemiology
- Cancer Research and Treatments
BOKU University
2015-2024
Institute of Molecular Biotechnology
2020-2024
Austrian Centre of Industrial Biotechnology (Austria)
2018-2019
Czech Academy of Sciences, Institute of Biotechnology
2009
University of Agricultural Sciences, Dharwad
2002
University of Agriculture Faisalabad
1996-2000
University of Nebraska–Lincoln
1990-1994
University of Chicago
1993
Recombinant production of therapeutically active proteins has become a central focus contemporary life science research. These are often produced in mammalian cells, order to obtain products with post-translational modifications similar their natural counterparts. However, cases where fast and flexible system for recombinant is needed, the use cells limited. The baculoviral insect cell proven be powerful alternative expression wide range short time frames. major drawback systems lies...
Based on the method of direct cloning into baculovirus genome by linearizing and re-ligation in presence target insert, we designed viral constructs that express foreign genes surface o particles. We chose glycosylated envelope protein gp41 human immunodeficiency virus type 1 (HIV-1) as a model for displaying recombinant budded virus. The ectodomain HIV-1 was being fused to entire major coa gp64 (Ac-cops41) membrane anchor sequence (Ac-mars41). Two different promoters, "very late" polyhedrin...
Lactobacillus plantarum constitutes a well-recognized food-grade system for the expression of recombinant proteins in field industrial and medical biotechnology. For applications vivo or biotechnological processes, level e.g. antigens enzymes is often critical, as levels should be certain effectiveness, yet, without putting too much strain to overall system. The key factors that control gene are promoter strength, copy number translation efficiency. In order estimate impact these adjusting...
Purpose of work: A comparative analysis new and established insect cell lines, in regard to process relevant parameters, provide data that can be exploited for designing more robust effective protein production processes. The baculovirus-insect expression system has been efficiently used the heterologous proteins. Three different lines Tnao38, High Five Sf9 were compared terms virus susceptibility, baculovirus product yield an intra-cellularly (YFP) extra-cellularly (influenza...
Human infections with a novel influenza A H7N9 subtype virus were reported in China recently. The caused severe disease high mortality rates and it raised concerns over its pandemic potential. Here, we assessed the mouse model protective efficacy of single immunisations low vaccine doses insect cell-derived H7 virus-like particles, consisting hemagglutinin matrix protein. Vaccinated mice fully protected survived stringent lethal challenge (100 mLD50) H7N9, even after single, unadjuvanted,...
Sheep pox virus (SPPV), goat (GTPV) and lumpy skin disease (LSDV) are very closely related viruses of the Capripoxvirus (CaPV) genus Poxviridae family. They responsible for sheep pox, which affect sheep, cattle, respectively. The epidemiology capripox diseases is complex, as some CaPVs not strictly host-specific. Additionally, three forms co-exist in many sub-Saharan countries complicates identification an outbreak. Genotyping using a low-cost, rapid, highly specific, easy to perform method...
Abstract Poxviruses belonging to the Orthopoxvirus, Capripoxvirus and Parapoxvirus genera share common host species create a challenge for diagnosis. Here, we developed novel multiplex PCR method simultaneous detection differentiation of eight poxviruses, three genera: cowpox virus (CPXV) camelpox (CMLV) [genus Orthopoxvirus ]; goatpox (GTPV), sheeppox (SPPV) lumpy skin disease (LSDV) orf (ORFV), pseudocowpox (PCPV) bovine papular stomatitis (BPSV) ]. The assay is based on high-resolution...
Orf is a contagious disease of sheep, goats and wild ungulates caused by orf virus (ORFV) member the genus Parapoxvirus, Poxviridae family. Although endemic in Ethiopia, little attention has been given so far as it not notifiable World Organization for Animal Health. In this work, we have investigated outbreaks representing five different geographical locations Amba Giorgis, Gondar zuria, Adet, Debre zeit Adami Tulu, between 2008 2013. The viral isolation sequence analysis A32L B2L genes...
Antibody tests are essential tools to investigate humoral immunity following SARS-CoV-2 infection or vaccination. While first-generation antibody have primarily provided qualitative results, accurate seroprevalence studies and tracking of levels over time require highly specific, sensitive quantitative test setups.
Vaccination against influenza virus can reduce the risk of by 40% to 60%, they rely on production neutralizing antibodies specific hemagglutinin (HA) ignoring neuraminidase (NA) as an important surface target. with standardized NA concentration may offer broader and longer-lasting protection infection. In this regard, we aimed compare potency a displayed VLP soluble NA. The baculovirus expression system (BEVS) novel virus-free Tnms42 insect cell line were used express N2 gag-based VLPs. To...
The baculovirus expression system was utilized to serve as a tool for ligand selection, demonstrating the applicability of generation and screening eukaryotic libraries. HIV-1-gp41 epitope 'ELDKWA', specific neutralizing human mAb 2F5, inserted into antigenic site B influenza virus hemagglutinin expressed on surface infected insect cells. In order improve antigenicity within hemagglutinin, therefore enhance binding we three additional, random amino acids adjacent epitope. This pool genes...
In order to release host cells from plasmid-mediated increases in metabolic load and high gene dosages, we developed a plasmid-free, T7-based E. coli expression system which the target is site-specifically integrated into genome of host. With this system, plasmid-loss, source instability for conventional systems, was eliminated. At same time, leakiness, challenging problem with recombinant minimized. The efficiency T7 RNA polymerase compensates low dosage provides rates without fatal...
Genetic modification of stem cells, prior to transplantation, can enhance their survival and improve function in cell therapy settings. Mesenchymal cells (MSC) are considered one the most promising tools for cell-based gene therapy, due multipotency, ease isolation, as well high ex vivo expansion potential. Neural (NSC) may also present an ideal route have been use replacement therapies various neurodegenerative diseases. Gene therapy-based applications require transfer genetic material,...