A5058954904- Gut microbiota and health
- Animal Nutrition and Physiology
- Antibiotic Resistance in Bacteria
- Clostridium difficile and Clostridium perfringens research
- Pharmaceutical and Antibiotic Environmental Impacts
- Salmonella and Campylobacter epidemiology
- Aquaculture disease management and microbiota
- Probiotics and Fermented Foods
- Ginseng Biological Effects and Applications
- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Enterobacteriaceae and Cronobacter Research
- Pharmacological Effects of Natural Compounds
- Diphtheria, Corynebacterium, and Tetanus
- Mycobacterium research and diagnosis
- Viral gastroenteritis research and epidemiology
- Meat and Animal Product Quality
- Antibiotics Pharmacokinetics and Efficacy
- Chemical Reactions and Isotopes
- GABA and Rice Research
- Gastrointestinal motility and disorders
- Vibrio bacteria research studies
- Microbial infections and disease research
- Infections and bacterial resistance
- Animal Behavior and Welfare Studies
Central European Institute of Technology
2020-2024
University of Veterinary Sciences Brno
2018-2022
Veterinary Research Institute
2014-2016
In this study we characterised the development of caecal microbiota in egg laying hens over their commercial production lifespan, from day hatching until 60 weeks age. Using pyrosequencing V3/V4 variable regions 16S rRNA genes for characterisation, were able to define 4 different stages development. The first stage lasted week life and was by a high prevalence Enterobacteriaceae (phylum Proteobacteria). second 2 nearly an absolute dominance Lachnospiraceae Ruminococcaceae (both phylum...
Since poultry is a very common source of non-typhoid Salmonella for humans, different interventions aimed at decreasing the prevalence in chickens are understood as an effective measure incidence human salmonellosis. One such intervention use probiotic or competitive exclusion products. In this study we tested whether microbiota from donor hens age will equally protect against Enteritidis infection. Newly hatched were therefore orally inoculated with cecal extracts 1-, 3-, 16-, 28-, and...
Introduction Hospitals and wastewater are recognized hot spots for the selection dissemination of antibiotic-resistant bacteria to environment, but total participation hospitals in spread nosocomial pathogens municipal treatment plants (WWTPs) adjacent rivers had not previously been revealed. Methods We used a combination culturing whole-genome sequencing explore transmission routes Escherichia coli from hospitalized patients suffering urinary tract infections (UTI) via environment. Samples...
Different housing systems can be used in pig production and little is known about their effect on gut microbiota composition. In this study we characterized fecal by sequencing the rRNA genes sows kept during gestation conventional pens with a slatted floor enriched covered deep straw. After farrowing, of 1- 4-day-old piglets were also monitored. Microbiota from system contained significantly more Prevotella, Parabacteroides, CF231, Phascolarctobacterium, Fibrobacter, Anaerovibrio YRC22 less...
Chickens are regarded as the main reservoir for human campylobacteriosis. Little is known about interaction between Campylobacter jejuni (C. jejuni) and chickens. This may be influenced by stage of maturation immune system, developing gut microbiota composition other factors including breed diet. Our aim was to investigate impact breed, diet on C. colonization host responses in Birds were inoculated with 104 colony forming units (CFU) or diluent at one (Exp. 1) 22 2) days post hatch. We...
After a ban on the use of antibiotics as growth promoters in farm animals European Union 2006, an interest alternative products with antibacterial or anti-inflammatory properties has increased. In this study, we therefore tested effects extracts from Curcuma longa and Scutellaria baicalensis used feed additives against cecal inflammation induced by heat stress Salmonella Enteritidis (S. Enteritidis) infection chickens. extract alone was not enough to decrease gut stress. However, mixture...
Since microbiota may influence the physiology of its host including body weight increase, growth rate or feed intake, in this study we determined composition high low residual intake (HRFI and LRFI) pig lines, different age and/or subjected to sanitary stress by sequencing V3/V4 variable region 16S rRNA genes. Allisonella, Megasphaera, Mitsuokella, Acidaminococcus (all belonging Firmicutes/class Negativicutes), Lactobacillus, Faecalibacterium, Catenibacterium, Butyrivibrio,...
One of the recent trends in animal production is revival interest organic farming. The increased consumer farming mainly due to concerns about welfare and use antibiotics conventional On other hand, providing animals with a more natural lifestyle implies their exposure environmental sources different microorganisms including pathogens. To address these concerns, we determined abundance antibiotic resistance diversity within fecal microbiota pigs kept under systems Sweden, Denmark, France...
International trade with ornamental fish is gradually recognized as an important source of a wide range different antibiotic resistant bacteria. In this study we therefore characterized the prevalence selected resistance genes in microbiota found carriage water originating from 3 continents. Real-time PCR quantification showed that sul1 gene was present 11 out 100 tet(A) 6 bacteria and strA, tet(G), sul2 aadA were 1–2 copies per Class I integrons quite common microbiota, however,...
In this study we determined protein and gene expression in the caeca of newly hatched chickens inoculated with cecal contents sourced from hens different ages. Over 250 proteins exhibited modified levels response to microbiota inoculation. The most significant inductions were observed for ISG12-2, OASL, ES1, LYG2, DMBT1-L, CDD, ANGPTL6, B2M, CUZD1, IgM Ig lambda chain. Of these, ES1 both immunoglobulins expressed at lower germ-free compared conventional chickens. contrast, CELA2A, BRT-2,...
The complete nucleotide sequences of six IMP-4-encoding plasmids recovered from Enterobacteriaceae isolates wildlife origin were characterized. Sequencing data showed that different incompatibility groups (IncM, IncI1, IncF, and nontypeable [including an IncX5_2 two pPrY2001-like]) carried the blaIMP-4-carrying integrons In809 or In1460. Most mph(A) region, chrA-like, aac(3)-IId, blaTEM-1b genes. Finally, plasmid analysis revealed involvement IS26- Tn1696-associated mechanisms in...
Multidrug-resistant (MDR) bacteria pose a significant challenge to the treatment of infectious diseases. Of particular concern are members Klebsiella pneumoniae species complex (KpSC), which frequently associated with hospital-acquired infections and have potential spread outside hospitals via wastewaters. In this study, we aimed investigate occurrence phylogenetic relatedness MDR KpSC from patients urinary tract (UTIs), hospital sewage, municipal wastewater plants (mWWTPs) surface waters...
Poultry represents a common source of bacteria with resistance to antibiotics including the critically important ones. Selective cultivation using colistin, cefotaxime and meropenem was performed for 66 chicken samples coming from 12 farms in Paraguay while two breeding companies supplied farms. A total 62 Escherichia coli 22 Klebsiella pneumoniae isolates were obtained representative subjected whole-genome sequencing. Relatively high prevalence phylogenetic group D F observed E. several...
Abstract Understanding disease aetiology and pathologic mechanisms is essential for fish health evaluation. Carp edema virus (CEV) the causative agent of a (CEVD) responsible high mortality rates in both wild cultured common carp Cyprinus carpio . Inspection two specimens from pond with revealed CEV infection host its ectoparasite ( Argulus foliaceus ). In addition to flavobacteria, well known be associated gill lesions, we found that free‐living eukaryotes (amoebae ciliates) temporary...
Wild corvids were examined for the presence of carbapenemase-producing Gram-negative bacteria in United States. A total 13 isolates detected among 590 fecal samples American crow; 11
This study represents follow-up research on three difficult-to-cultivate anaerobic isolates originally isolated within a project focused strains that are able to stably colonize newly hatched chickens, thus representing possible probiotics. is exceptional in it successfully several miscellaneous required modified and richly supplemented media, as information many gut-colonizing bacteria based predominantly metagenomic studies.