A5066572928- Antibiotic Resistance in Bacteria
- Enterobacteriaceae and Cronobacter Research
- Bacterial Identification and Susceptibility Testing
- Pharmaceutical and Antibiotic Environmental Impacts
- Bacterial biofilms and quorum sensing
- Mycobacterium research and diagnosis
- Vibrio bacteria research studies
- Antimicrobial Resistance in Staphylococcus
- Infections and bacterial resistance
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Use and Resistance
- Pneumonia and Respiratory Infections
- Genomics and Phylogenetic Studies
- Urinary Tract Infections Management
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
- Biosensors and Analytical Detection
- Insect Resistance and Genetics
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Bacterial Infections and Vaccines
- Infective Endocarditis Diagnosis and Management
- Escherichia coli research studies
- Streptococcal Infections and Treatments
- Insect and Pesticide Research
Charles University
2016-2025
Opća bolnica Karlovac
2020
National Institute of Public Health
2020
Istituto Superiore di Sanità
2020
Czech Academy of Sciences, Institute of Microbiology
2019
University Hospital Plzen
2010-2017
University of Veterinary Sciences Brno
2017
Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry is used for the determination molecular weights different chemical compounds. We describe here use MALDI-TOF to detect a carbapenem antibiotic, meropenem, and its degradation products. Buffered meropenem solution (0.1 mM Tris-HCl, pH 6.8) was mixed with an overnight culture bacteria. After 3-h incubation, reaction mixture centrifuged, supernatant analyzed by spectrometry. The presence or absence peaks...
Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) is a potentially useful tool for the detection antimicrobial resistance, especially that conferred by β-lactamases. Here we describe modification previously reported MALDI-TOF MS meropenem hydrolysis assay. The modified method was validated on 108 carbapenemase-producing members Enterobacteriaceae, two NDM-1-producing Acinetobacter baumannii isolates, and 35 carbapenem-resistant enterobacteria...
Significance In many clinically important bacteria, antibiotic resistance genes are primarily carried on plasmids. These can spread horizontally between different strains and species. However, current surveillance systems track chromosomal lineages of bacteria only, leading to an incomplete understanding how spreads, from within individual hospital across country borders. We present integrated, high-resolution analysis both chromosome plasmid sequences using Klebsiella pneumoniae isolates...
ABSTRACT A comparison of a matrix-assisted laser desorption ionization–time flight mass spectrometric (MALDI-TOF MS) meropenem hydrolysis assay with the Carba NP test showed that both methods exhibited low sensitivity (approximately 76%), mainly due to false-negative results obtained OXA-48-type producers. The addition NH 4 HCO 3 reaction buffer for MALDI-TOF MS dramatically improved its (98%). Automatic interpretation assay, using MBT STAR-BL software, generally agreed after manual...
BackgroundMorganella spp are opportunistic pathogens involved in various infections. Intrinsic resistance to multiple antibiotics (including colistin) combined with the emergence of carbapenemase producers reduces number active antimicrobials. The aim this study was characterise genetic features related spread carbapenem-resistant Morganella spp.MethodsThis comparative genomic included extensively drug-resistant isolates collected between Jan 1, 2013, and March 2021, by French National...
The aim of this study was to characterize the first cases and outbreaks OXA-48-like-producing Enterobacteriaceae recovered from hospital settings in Czech Republic. From 2013 2015, 22 Klebsiella pneumoniae isolates, 3 Escherichia coli 1 Enterobacter cloacae isolate producing OXA-48-like carbapenemases were isolated 20 patients. Four patients colonized or infected by two three different producers. K. isolates classified into nine sequence types (STs), with ST101 being predominant (n = 8). E....
The aim of the present study was to characterize sporadic cases and an outbreak NDM-like-producing Enterobacteriaceae recovered from hospital settings, in Czechia. During 2016, 18 Entrobacteriaceae isolates including 10 Enterobacter cloacae complex (9 E. xiangfangensis 1 asburiae), 4 Escherichia coli, Kluyvera intermedia, Klebsiella pneumoniae, oxytoca, Raoultella ornithinolytica that produced NDM-like carbapenemases were isolated 15 patients. Three patients colonized or infected by two...
Carbapenem-resistant Acinetobacter baumannii (CRAB) has been implicated in hospital outbreaks worldwide. Here, we present a whole-genome-based investigation of an extensively drug-resistant CRAB outbreak rapidly spreading and causing high incidences mortality at numerous wards large tertiary Lebanon. This is the first study its kind region. Two circulating clones were identified using combination molecular typing approaches, short- long-read sequencing Bayesian transmission network analysis....
The ability of MALDI-TOF for the identification nontuberculous mycobacteria (NTM) has improved recently thanks to updated databases and optimized protein extraction procedures. Few multicentre studies on reproducibility have been performed so far, none mycobacteria. aim this study was evaluate NTM in 15 laboratories 9 European countries. A total 98 clinical isolates were grown Löwenstein-Jensen. Biomass collected tubes with water ethanol, anonymized sent out participating laboratories....
ABSTRACT The objective of this study was to perform molecular surveillance for assessing the spread carbapenemase-producing Pseudomonas aeruginosa in Czech hospitals. One hundred thirty-six isolates were recovered from 22 hospitals located throughout country. Sequence type 357 (ST357) dominated ( n = 120) among carbapenemase producers. seventeen produced IMP-type (IMP-7 [ 116] and IMP-1 1]) metallo-β-lactamases (MβLs), 15 VIM-2 MβL, remaining expressed GES-5 enzyme. bla IMP -like genes three...
Silver gulls carry phylogenetically diverse Escherichia coli, including globally dominant extraintestinal pathogenic E. coli (ExPEC) sequence types and pandemic ExPEC-ST131 clades; however, our large-scale study (504 samples) on silver nesting off the coast of New South Wales identified ST457 as most prevalent. A phylogenetic analysis whole-genome sequences (WGS) 138 samples comprising 42 from gulls, 2 humans (Australia), 14 poultry farmed in Paraguay were compared with 80 WGS deposited...
The aim of this study was to characterize four Enterobacterales co-producing NDM- and OXA-48-like carbapenemases from Czech patients with travel history or/and previous hospitalization abroad. Klebsiella pneumoniae isolates belonged “high risk” clones ST147, ST11, ST15, while the Escherichia coli isolate assigned ST167. All expressed resistance against most β-lactams, including carbapenems, retaining susceptibility colistin. Furthermore, analysis WGS data showed that all co-produced OXA-48-...
Abstract Objectives The objective of this study was to perform a multinational survey patients' colonization by metallo-β-lactamase (MBL)-producing Enterobacteriaceae, including their molecular characterization. Methods Patients in 18 hospital units across Europe and Israel (n = 17 945) were screened between mid-2008 mid-2011. MBL-producing isolates typed PFGE MLST. MBL genes amplified sequenced within integrons. Plasmids with analysed nuclease S1 plus hybridization profiling, mating...
Abstract Colistin is considered as a last resort treatment for infections caused by multidrug-resistant Enterobacteriaceae. Plasmid-mediated mobile colistin resistance ( mcr ) genes contributed to the global spread of resistance. This first report plasmid-mediated mcr-8 gene from clinical Klebsiella pneumoniae K9 isolate recovered Lebanon. The was characterized phenotypically and genotypically through both short long read whole-genome sequencing, plasmid typing conjugation assays. k9...
Infections caused by carbapenemase-producing bacteria have led to the revival of polymyxins as “last-resort” antibiotic. Since 2016, several reports describing presence plasmid-mediated colistin resistance genes, mcr , in different host species and geographic areas were published.
A 2-month survey of extended-spectrum beta-lactamase (ESBL) producers was performed in a Czech hospital. Klebsiella pneumoniae produced SHV-2, -5, or -12, Escherichia coli CTX-M-9 -15, and other species TEM-92 -132. All K. E. isolates belonged to sequence types (STs) clonal complexes (CCs) spread across the world (K. complex 11 [CC11], CC14, type 101 [ST101] CC31, CC73, CC131, CC405) carried various plasmids (mainly with A/C- FII-type replicons).
We describe the isolation of an NDM-1-producing Acinetobacter baumannii in a Czech patient repatriated July 2011 from Egypt. The infection spread to another on same ward. Both isolates showed resistance pattern and were susceptible only colistin. They had identical PFGE belonged sequence type ST 1. Sequencing blaNDM gene identified NDM-1 variant carbapenemase, surrounded by two copies insertion ISAba125.
Here, we describe two plasmids carrying mcr-4.3 in Acinetobacter baumannii strains isolated from imported food and a clinical sample. The comparative analysis of these plasmids, with other reported the NCBI database, highlighted common origin plasmidic structure This is first case gene A. strain Europe. We hypothesize that import initiating spread Czech Republic.
The aim of the study was to develop an orthopedic implant coating in form vancomycin-loaded collagen/hydroxyapatite layers (COLHA+V) that combine ability prevent bone infection with promote enhanced osseointegration. investigated employing a rat model simulated clinically relevant implant-related introduction bacterial contamination during surgical procedure using clinical isolate Staphylococcus epidermidis. enhance osseointegration minipig terminated growth. Six weeks following...