A5034763831- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Enterobacteriaceae and Cronobacter Research
- Pharmaceutical and Antibiotic Environmental Impacts
- Infections and bacterial resistance
- Antibiotic Use and Resistance
- Antimicrobial Resistance in Staphylococcus
- Pneumonia and Respiratory Infections
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Plant Pathogenic Bacteria Studies
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
- Genomics and Phylogenetic Studies
- Biosensors and Analytical Detection
- Mycobacterium research and diagnosis
- Urinary Tract Infections Management
- SARS-CoV-2 and COVID-19 Research
- Enzyme Structure and Function
- SARS-CoV-2 detection and testing
- Evolution and Genetic Dynamics
Université Paris-Saclay
2016-2025
Inserm
2011-2025
CEA Paris-Saclay
2020-2025
Commissariat à l'Énergie Atomique et aux Énergies Alternatives
2020-2025
CNR de la Résistance aux Antibiotiques
2016-2025
Bicêtre Hospital
2016-2025
Assistance Publique – Hôpitaux de Paris
2016-2025
Infectious Disease Models and Innovative Therapies
2022-2024
Microbe, Intestine, Inflammation and Host Susceptibility
2022-2024
Emera (Canada)
2024
We previously isolated two human L1 elements (L1.2 and LRE2) as the progenitors of disease-producing insertions. Here, we show these can actively retrotranspose in cultured mammalian cells. When stably expressed from an episome HeLa cells, both retrotransposed into a variety chromosomal locations at high frequency. The products resembled endogenous insertions, since they were variably 5′ truncated, ended poly(A) tracts, flanked by target-site duplications or short deletions. Point mutations...
ABSTRACT Pseudomonas aeruginosa COL-1 was identified in a blood culture of 39-year-old-woman treated with imipenem Marseilles, France, 1996. This strain resistant to β-lactams, including ureidopenicillins, ticarcillin-clavulanic acid, cefepime, ceftazidime, imipenem, and meropenem, but remained susceptible the monobactam aztreonam. The carbapenem-hydrolyzing β-lactamase gene P. cloned, sequenced, expressed Escherichia coli DH10B. deduced 266-amino-acid protein an Ambler class B β-lactamase,...
Beta-Lactamase Database (BLDB) is a comprehensive, manually curated public resource providing up-to-date structural and functional information focused on this superfamily of enzymes with great impact antibiotic resistance. All the reported characterised in literature are presented according to class (A, B, C D), family subfamily which they belong. three-dimensional structures β-lactamases present Protein Data Bank also shown. The characterisation representative mutants hydrolytic profiles...
Genetic structures surrounding the carbapenem-hydrolyzing Ambler class A bla KPC gene were characterized in several KPC-positive Klebsiella pneumoniae and Pseudomonas aeruginosa strains isolated from United States, Colombia, Greece. The genes associated all cases with transposon-related structures. In K. YC isolate beta-lactamase KPC-2 was located on a novel Tn3-based transposon, Tn4401. Tn4401 10 kb size, delimited by two 39-bp imperfect inverted repeat sequences, harbored, addition to...
ABSTRACT Klebsiella pneumoniae ORI-1 was isolated in 1998 France from a rectal swab of 1-month-old girl who previously hospitalized Cayenne Hospital, Cayenne, French Guiana. This strain harbored ca. 140-kb nontransferable plasmid, pTK1, that conferred an extended-spectrum cephalosporin resistance profile antagonized by the addition clavulanic acid, tazobactam, or imipenem. The gene for GES-1 (Guiana β-lactamase) cloned, and its protein expressed Escherichia coli DH10B, where this pI-5.8...
Abstract To assess dissemination of OXA-23–producing strains Acinetobacter baumannii, we obtained 20 carbapenem-resistant, isolates from different regions. Their clonal relationship was assessed by pulsed-field gel electrophoresis and multilocus sequence typing. We identified 8 types, including 4 novel types. All except 2 belonged to main European lineages. The blaOXA-23 gene either located on the chromosome or plasmids associated with genetic structures.
Klebsiella pneumoniaeisolates that produce carbapenemases (KPCs) are rapidly disseminating worldwide. To determine their genetic background, we investigated 16 blaKPC-2-harboring K. pneumoniae isolates from 5 countries. The were multidrug resistant, possessed the blaKPC-2 gene, and differed by additional Beta-lactamase content. They harbored a naturally chromosome-encoded bla gene (blaSHV-1 [12.5%], blaSHV-11 [68.7%], or blaOKP-AVB [18.8%]) several acquired plasmid-encoded genes (blaTEM-1...
The global spread of carbapenemase-producing Enterobacteriaceae represents a substantial challenge in clinical practice and rapid reliable detection these organisms is essential. aim this study was to develop validate lateral flow immunoassay (Carba5) for the five main carbapenemases (KPC-, NDM-, VIM- IMP-type OXA-48-like). Carba5 retrospectively prospectively evaluated using 296 enterobacterial isolates from agar culture. An isolated colony suspended extraction buffer then loaded on...
ABSTRACT Pseudomonas aeruginosa GW-1 was isolated in 2000 South Africa from blood cultures of a 38-year-old female who developed nosocomial pneumonia. This isolate harbored self-transferable ca. 100-kb plasmid that conferred an expanded-spectrum cephalosporin resistance profile associated with intermediate susceptibility to imipenem. A β-lactamase gene, bla GES-2 , cloned whole-cell DNA P. and expressed Escherichia coli . GES-2, pI value 5.8, hydrolyzed cephalosporins, its substrate extended...
ABSTRACT A clinical isolate, Escherichia coli MG-1, isolated from a 4-month-old Vietnamese orphan child, produced β-lactamase conferring resistance to extended-spectrum cephalosporins and aztreonam. In disk diffusion test, typical synergistic effect between ceftazidime or aztreonam clavulanic acid was observed along with an unusual synergy cefoxitin cefuroxime. The gene for VEB-1 (Vietnamese β-lactamase) cloned expressed in E. JM109. recombinant plasmid pRLT1 pI of 5.35 conferred high-level...
ABSTRACT Further characterization of the genetic environment gene encoding Escherichia coli extended-spectrum β-lactamase, bla VEB-1 , revealed presence a plasmid-located class 1 integron, In53, which carried eight functional resistance cassettes in addition to . While aadB and arr-2 were identical those previously described, remaining novel: (i) novel nonenzymatic chloramphenicol cmlA family, (ii) qac allele member small multidrug family proteins, (iii) cassette, aacA1b/orfG, encodes 6′- N...
The genetic structures surrounding the plasmid-carried blaOXA-23 oxacillinase gene, encoding resistance to carbapenems, were studied in Acinetobacter baumannii. ISAba1 and novel element ISAba4 detected upstream of providing promoter sequences for its expression. These insertion elements likely involved transposition processes at origin acquisition this beta-lactamase gene.
Clinical isolate Pseudomonas aeruginosa Mus showed resistance both to extended-spectrum cephalosporins and aztreonam. We detected a typical double-disk synergy image when ceftazidime or aztreonam was placed next clavulanic acid disk on an agar plate. This phenotype suggested the presence of beta-lactamase. Isoelectric focusing revealed that this strain produced three beta-lactamases, pI 5.5, 7.4, 8.2. A 2.6-kb Sau3A fragment encoding beta-lactamase 5.5 cloned from P. genomic DNA. enzyme,...