A5015194301- Aquaculture disease management and microbiota
- Myxozoan Parasites in Aquatic Species
- Insect and Pesticide Research
- Parasite Biology and Host Interactions
- Aquaculture Nutrition and Growth
- Insect and Arachnid Ecology and Behavior
- Fish Ecology and Management Studies
- Invertebrate Immune Response Mechanisms
- Environmental Toxicology and Ecotoxicology
- Plant and animal studies
- Aquatic Invertebrate Ecology and Behavior
- Microbial infections and disease research
- Biochemical effects in animals
- Nitric Oxide and Endothelin Effects
- Receptor Mechanisms and Signaling
- Electron Spin Resonance Studies
- Aquatic Ecosystems and Phytoplankton Dynamics
- Physiological and biochemical adaptations
- Eicosanoids and Hypertension Pharmacology
- Cardiac Ischemia and Reperfusion
- Gout, Hyperuricemia, Uric Acid
- Animal Virus Infections Studies
- Neuropeptides and Animal Physiology
- Viral Infections and Vectors
- Bat Biology and Ecology Studies
University of Veterinary Sciences Brno
2015-2025
Mendel University in Brno
2020-2025
Czech Academy of Sciences, Institute of Vertebrate Biology
2021
Czech Academy of Sciences, Institute of Biophysics
2006-2012
Czech Academy of Sciences
2001-2012
Endothelial dysfunction characterized by decreased nitric oxide (NO) bioavailability is the first stage of coronary artery disease. It known that one factors associated with an increased risk disease a high plasma level uric acid. However, causative associations between hyperuricaemia and cardiovascular have not been definitely proved. In this work, we tested effect acid on endothelial NO bioavailability. Electrochemical measurement production in acetylcholine-stimulated human umbilical...
Carp Edema Virus (CEV) has emerged as a viral threat to the sustainability of European pond fisheries, with water temperature and stress playing crucial role in disease outbreaks. Here, we report on natural CEV infection overwintering common carp ( Cyprinus carpio ; n = 1,160) broodstock that began manifest clinically at an unusually low temperature. In initial outbreak phase, young fish exhibited abnormal activity shoaling edge. While under discontinuous thin ice layer was 2°C, no deaths...
The aim of this study was to determine phenotypic and genotypic resistance, virulence clonal relationship aeromonads related species isolated from Czech carp fisheries. Forty‐nine isolates obtained a total 154 fish three breeding facilities were identified using matrix‐assisted laser desorption/ionization time flight the sequencing rpoB housekeeping gene. Most Aeromonas as veronii (94%, n = 34). Susceptibility six antibiotics (oxytetracycline, flumequine, florfenicol,...
Abstract This work describes the first confirmed cases of carp oedema virus disease (CEVD) in Slovakia and Czech Republic phylogenetic analysis Slovak (CEV) isolates. Four outbreak are described, oldest dating from mid‐May 2013 one case May 2019. In all cases, presence was using nested PCR. PCR products were sequenced compared with 357‐bp nucleotide sequences encoding CEV P4a protein GenBank. four (three common breeding facilities koi garden pond), detected belonged to genogroup I. (koi fish...
Here, we study the effect of oxalic acid on isolated varroa mites and parasitizing caged honey bees treated with per os or topically (by trickling by sublimation). We also (trickling sublimation) individual bees, focusing their lifespan, midgut morphology function, Malpighian tubule morphology. Effect mites: contact coated surface (Petri dishes significantly decreased mite viability. In an experiment acid, strongest acaricidal was observed following oral application lowest when applied...
Significant mortalities associated with emerging viral diseases are challenging the economy of common carp aquaculture. As such, there is an increased need to disentangle how infected fish cope progressive disease pathology and lose ability for homeostatic maintenance key physiological parameters. A natural edema virus (CEV) infection outbreak at a farm provided opportunity examine diseased healthy in same storage pond, thereby contributing our better understanding CEV pathophysiology. The...
Here, we present the results of a 2-year field trial aimed at testing effect overwintering on different feeds course Nosema ceranae infection. In August 2015, four experimental bee colony groups were established. After last honey harvest, each was provided with 20 kg feed, either honey, sugar (3:2 solution in tap water), inverted syrup made sucrose, or wheat starch syrup. Samples live bees collected from beehive (before feeding), November, and May. The following year, feeding sampling...
The aim of this study was to investigate whether haemocytes Galleria mellonella (Lepidoptera: Pyralidae) larvae produce reactive oxygen species (ROS) like human blood phagocytes.The production ROS measured first using luminolenhanced chemiluminescence un-stimulated and stimulated (four activators with different modes action) haemolymph or isolated haemocytes.However, spontaneous activated remained at the background level.In subsequent experiments an ultrasensitive fluorescence method Amplex...
Abstract Understanding disease aetiology and pathologic mechanisms is essential for fish health evaluation. Carp edema virus (CEV) the causative agent of a (CEVD) responsible high mortality rates in both wild cultured common carp Cyprinus carpio . Inspection two specimens from pond with revealed CEV infection host its ectoparasite ( Argulus foliaceus ). In addition to flavobacteria, well known be associated gill lesions, we found that free‐living eukaryotes (amoebae ciliates) temporary...
Disease conditions that involve multiple predisposing or contributing factors, manifest as low performance and/or low-level mortality, can pose a diagnostic challenge requires an interdisciplinary approach. Reaching diagnosis may also be limited by lack of available clinical profile parameter reference ranges to discriminate healthy fish from those affected specific disease conditions. Here, we describe our experience investigating poorly performing rainbow trout ( Oncorhynchus mykiss ) in...
In the present study, we describe a natural outbreak of carp edema virus disease (CEVD) in koi carp, concentrating on clinical manifestation, gross and microscopic pathology, immunological parameters, viral diagnostics, phylogenetic analysis. Examination white blood cell parameters showed increased monocyte decreased lymphocyte counts CEV-affected fish compared to healthy control fish. Regarding immune system functioning, work shows, for first time, enhanced phagocytic activity Respiratory...
Honey bee viruses have been shown to negatively affect the vigour and longevity of European honey bees (
The heterotherm immune system undergoes significant variation in response to life cycle periodicity and torpor. As heterothermic bats are important reservoirs of zoonotic agents modulation activity can affect host-pathogen interactions, this work aimed at developing a suitable method for assessing phagocyte activity. Chemiluminescence measurements were evaluated by mathematical mechanistic approaches, both which yielded comparable results time-related parameters Using method, however, we...
Abstract Background Emergence of both viral zoonoses from bats and diseases that threaten bat populations has highlighted the necessity for greater insights into functioning immune system. Particularly when considering hibernating temperate species, it is important to understand seasonal dynamics associated with response. Body temperature one factors modulates functions defence mechanisms against pathogenic agents in vertebrates. To better innate immunity mediated by phagocytes bats, we...
The population of brown trout (Salmo trutta fario) in continental Europe is on the decline, with infectious diseases confirmed as one causative factors. However, no data epizootiological situation wild fish Czech Republic are currently available. In this study, (n = 260) from eight rivers were examined for presence viral and parasitical pathogens. Salmonid alphavirus-2, pancreatic necrosis virus, piscine novirhabdovirus (VHSV) salmonid (IHNV) not detected using PCR. Cell culturing showed...
Fish can be exposed to multiple stressors at the same time, including natural toxins, environmental pollutants or feed contamination. In this study, we report on effects of controlled exposure rainbow trout ( Oncorhynchus mykiss ) stressors. doing so, test hypothesis that cyanobacterial biomass and arsenic in combine enhance negative fish, modulation antioxidant response. The (mean weight 288 ± 59 g) were divided into six experimental groups 25 fish each (C - control group; B 3% lyophilised...