A5090976729- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Infections and bacterial resistance
- Antibiotics Pharmacokinetics and Efficacy
- Enterobacteriaceae and Cronobacter Research
- Escherichia coli research studies
- Plant Pathogenic Bacteria Studies
- Antimicrobial Resistance in Staphylococcus
- Pharmaceutical and Antibiotic Environmental Impacts
- Genomics and Phylogenetic Studies
- Bacterial Identification and Susceptibility Testing
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Streptococcal Infections and Treatments
- Bacterial Genetics and Biotechnology
- Infective Endocarditis Diagnosis and Management
- Mycobacterium research and diagnosis
- Salmonella and Campylobacter epidemiology
- Drug Transport and Resistance Mechanisms
- Pneumonia and Respiratory Infections
- Microbial Applications in Construction Materials
- Cystic Fibrosis Research Advances
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
California State University, Fullerton
2016-2025
Marshall B. Ketchum University
2024
Louis Stokes Cleveland VA Medical Center
2021-2023
Geriatric Research Education and Clinical Center
2021-2023
Consejo Nacional de Investigaciones Científicas y Técnicas
2012-2023
Centro Científico Tecnológico - San Juan
2019
University of Buenos Aires
2006-2016
Universidad Peruana Cayetano Heredia
2012
Universidade Federal do Rio Grande do Sul
2005
Pontifícia Universidade Católica do Rio Grande do Sul
2004
REVIEW article Front. Microbiol., 17 May 2013Sec. Antimicrobials, Resistance and Chemotherapy Volume 4 - 2013 | https://doi.org/10.3389/fmicb.2013.00121
Acinetobacter baumannii A118 was isolated from a patient's blood culture. It is susceptible to several antibiotics, naturally competent, and supports replication stable maintenance of four plasmid replicons. A. took up fluorophore-labeled oligonucleotide analog. These characteristics make this isolate convenient model for genetic studies.
In recent years, MALDI-TOF Mass Spectrometry (MS) method has emerged as a promising and reliable tool for bacteria identification. this study we compared Bruker MS conventional phenotypic methods to identify collection of 333 Gram-positive clinical isolates comprising 22 genera 60 species. 16S rRNA sequencing was the reference molecular technique, rpoB gene sequecing used secondary target when 16Sr RNA did not allow species identification Corynebacterium spp. We also investigate if score...
Staphylococcus aureus (SA) and Pseudomonas aeruginosa (PA) cause a wide variety of bacterial infections coinfections, showing complex interaction that involves the production different metabolites metabolic changes. Temperature is key factor for survival virulence within host, bacteria could be exposed to an increment in temperature during fever development. We analyzed previously unexplored effect fever-like temperatures (39 °C) on S. USA300 P. PAO1 microaerobic mono- co-cultures compared...
Background Class 1 integrons are one of the most successful elements in acquisition, expression and spread antimicrobial resistance genes (ARG) among clinical isolates. Little is known about gene flow components genetic platforms class within between bacterial communities. Thus it important to better understand interactions "environmental" intI1, its distribution with human activities. Methodology/Principal Findings An evaluation two types determinants, ARG (sul1 qacE1/qacEΔ1 genes) lateral...
ABSTRACT We report the first case of fulminant sepsis due to Wohlfahrtiimonas chitiniclastica . This is also one reported in South America. emphasize importance recognizing bacteria that live larvae a parasitic fly as causative agent severe infections homeless patients.
Recent studies have demonstrated a high genomic plasticity in Acinetobacter baumannii , which may explain its capacity to acquire multiple antibiotic resistance determinants and survive the hospital environment. strain A118 ( Ab A118) was isolated year 1995 from blood culture of an intensive care unit patient. As this particular showed some peculiar characteristic such as being naturally competent susceptible numerous antibiotics, we performed whole-genome comparison (WGC) gain insights into...
Abstract In the past few decades Acinetobacter baumannii has emerged as a notorious nosocomial pathogen because of its ability to acquire genetic material and persist in extreme environments. Recently, human serum albumin (HSA) was shown significantly increase natural transformation frequency A. . This observation led us perform transcriptomic analysis strain A118 under HSA induction identify genes that are altered by HSA. Our results revealed statistically significant differential...
Abstract In a recent report by the Centers for Disease Control and Prevention (CDC), multidrug resistant (MDR) Acinetobacter baumannii is pathogen described as an “urgent threat.” Infection with this bacterium manifests different diseases such community nosocomial pneumonia, bloodstream infections, endocarditis, infections of urinary tract, wound burn skin soft tissue meningitis. particular, meningitis, unwelcome complication neurosurgery caused extensively-drug (XDR) A. , extremely...
Colistin is an antibiotic of last resort for treating serious Gram-negative bacterial infections. However, the misuse colistin, especially as animal growth promoter, has contributed to increasing antimicrobial resistance, mediated mainly through plasmid transfer mcr-1 gene. This study assessed prevalence phenotypic and molecular colistin resistance in Escherichia coli Klebsiella pneumoniae Ecuador healthy humans their chickens pigs.Fecal samples were collected from pigs two rural coastal...
Background: In order to study the enzymatic carbapenem resistance mechanisms in Acinetobacter baumannii isolates from Argentina, we performed molecular characterization on 41 epidemiologically unrelated strains isolated 1995 2006 with diminished susceptibilities imipenem and meropenem. Methodology: were identified ARDRA technique. The total genomic DNA was used detect each β-lactamase gene described so far this species those insertion sequences usually associated genes (ISAba1, 2, 3, 4 IS18)...
In the last few years, numerous cases of multidrug-resistant Achromobacter xylosoxidans infections have been documented in immunocompromised and cystic fibrosis patients. To gain insights into molecular mechanisms mobile elements related to multidrug resistance this bacterium, we studied 24 non-epidemiological A. clinical isolates from Argentina. Specific primers for plasmids, transposons, insertion sequences, bla(ampC), intI1, intI2 genes were used PCR reactions. The obtained results showed...
ABSTRACT Herein, we report four cases of Comamonas kerstersii intra-abdominal infections representing the first human caused by this species. In addition, our work demonstrates association C. with peritonitis secondary to appendix rupture.
The increasing frequency of bacteria showing antimicrobial resistance (AMR) raises the menace entering into a postantibiotic era. Horizontal gene transfer (HGT) is one prime reasons for AMR acquisition. Acinetobacter baumannii nosocomial pathogen with outstanding abilities to survive in hospital environment and acquire determinants. Its capacity incorporate exogenous DNA major source genes; however, few studies have addressed this subject. transformation machinery as well factors that induce...
In vitro activity of the aminoglycoside 6'-N-acetyltransferase type Ib [AAC(6')-Ib] was inhibited by ZnCl2 with a 50% inhibitory concentration (IC50) 15 μM. Growth Acinetobacter baumannii or Escherichia coli harboring aac(6')-Ib in cultures containing 8 μg/ml amikacin significantly addition 2 μM Zn(2+) complex ionophore pyrithione (ZnPT).
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Acinetobacter baumannii is a feared, drug-resistant pathogen, characterized by its ability to resist extreme environmental and nutrient-deprived conditions. Previously, we showed that human serum albumin (HSA) can increase foreign DNA acquisition specifically alter the expression of genes associated with pathogenicity. Moreover, in recent genome-wide transcriptomic study, observed pleural fluid (PF), an HSA-containing fluid, increases acquisition, modulate cytotoxicity, control immune...