A5007847789- Heat shock proteins research
- Protein Structure and Dynamics
- Endoplasmic Reticulum Stress and Disease
- Genetics, Aging, and Longevity in Model Organisms
- Computational Drug Discovery Methods
- Invertebrate Immune Response Mechanisms
- Schizophrenia research and treatment
- T-cell and B-cell Immunology
- Spaceflight effects on biology
- RNA Research and Splicing
- Polysaccharides and Plant Cell Walls
- Aquaculture disease management and microbiota
- Legume Nitrogen Fixing Symbiosis
- Nematode management and characterization studies
- Wnt/β-catenin signaling in development and cancer
- Biochemical effects in animals
- Immune Cell Function and Interaction
- RNA modifications and cancer
- Animal Disease Management and Epidemiology
- Plant-Microbe Interactions and Immunity
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Kruppel-like factors research
- Genetic diversity and population structure
- Ion Channels and Receptors
California State University, Fullerton
2015-2025
University of Illinois Chicago
2024
Hebrew University of Jerusalem
2024
Pennsylvania State University
2004-2013
Aristotle University of Thessaloniki
1998-2013
Papageorgiou General Hospital
2013
Intercollege
2009
Tri-County Community Mental Health Center
2008
Park University
2005
The cellular stress response (CSR) is a conserved mechanism that protects cells from -environmental and physiological stressors. heat shock (HSR), critical component of the CSR, utilizes molecular chaperones to mitigate proteotoxic caused by elevated temperatures. We hypothesized while canonical HSR pathways are across cell types, specific lines may exhibit unique transcriptional responses shock. To test this, we compared transcriptomic HEK293, HepG2, HeLa under control conditions...
We solved the crystal structure of a secreted protein, EXLX1, encoded by yoaJ gene Bacillus subtilis . Its is remarkably similar to that plant β-expansins (group 1 grass pollen allergens), consisting 2 tightly packed domains (D1, D2) with potential polysaccharide-binding surface spanning domains. Domain D1 has double-ψ β-barrel fold partial conservation catalytic site found in family 45 glycosyl hydrolases and MltA lytic transglycosylases. D2 an Ig-like group 2/3 allergens, structural...
REVIEW article Front. Microbiol., 17 May 2013Sec. Antimicrobials, Resistance and Chemotherapy Volume 4 - 2013 | https://doi.org/10.3389/fmicb.2013.00121
We made use of EXLX1, an expansin from Bacillus subtilis, to investigate protein features essential for its plant cell wall binding and loosening activities. found that the two domains, D1 D2, need be linked extension activity D2 mediates EXLX1 whole walls cellulose via distinct residues on surface. Binding is mediated by three aromatic arranged linearly putative surface spans D2. Mutation these alanine eliminated concomitantly measured either or weakening filter paper but hardly affected...
Although endogenous retroviruses are common across vertebrate genomes, the koala retrovirus (KoRV) is only known to be currently invading germ line of its host. KoRV believed have first infected koalas in northern Australia less than two centuries ago. We examined 28 museum skins collected late 19th and 20th deep sequenced complete proviral envelope region from five Australian specimens. Strikingly, env sequences were conserved among over span a century, functional motifs that affect viral...
Horizontal gene transfer (HGT) has been described as a common mechanism of transferring genetic material between prokaryotes, whereas transfers from eukaryotes to prokaryotes have rarely documented. Here we report rare case HGT in which plant expansin genes that code for cell-wall loosening proteins were transferred plants bacteria, fungi, and amoebozoa. In several cases, the species was found is either intimate association with or known pathogen. Our analyses suggest at least two...
Background: We previously showed that cardiomyocyte Krϋppel-like factor (KLF) 5 regulates cardiac fatty acid oxidation. As heart failure has been associated with altered oxidation, we investigated the role of KLF5 in lipid metabolism and pathophysiology ischemic failure. Methods: Using real-time polymerase chain reaction Western blot, expression changes a myocardial infarction (MI) mouse model tissue from patients 2D echocardiography, evaluated effect inhibition after MI using...
Germline colonization by retroviruses results in the formation of endogenous (ERVs). Most colonization’s occurred millions years ago. However, Australo-Papuan region (Australia and New Guinea), several recent germline events have been discovered . The Wallace Line separates much Southeast Asia from restricting faunal pathogen dispersion. West Line, gibbon ape leukemia viruses (GALVs) isolated captive gibbons. Two microbat species China appear to infected naturally. East Wallace’s woolly...
We have identified the Hsp70 gene superfamily of nematode Caenorhabditis briggsae and investigated evolution these genes in comparison with from C. elegans, Drosophila, yeast. The are classified into three monophyletic groups according to their subcellular localization, namely, cytoplasm (CYT), endoplasmic reticulum (ER), mitochondria (MT). Hsp110 can be polyphyletic CYT group ER group. different appeared evolve following model divergent evolution. This also explain MT genes. On other hand,...
In eukaryotes, the assembly and elongation of unbranched actin filaments is controlled by formins, which are long, multidomain proteins. These proteins important for dynamic cellular processes such as determination cell shape, division, interaction. Yet, no comprehensive study has been done about origins evolution this gene family. We therefore performed extensive phylogenetic motif analyses formin genes examining 597 prokaryotic 53 eukaryotic genomes. Additionally, we used three-dimensional...
The phylogenetic relationships of Ig light chain ( IGL ) genes are difficult to resolve, because these short and evolve relatively fast. Here, we classify the sequences from 12 tetrapod species into three distinct groups (κ, λ, σ isotypes) using conserved amino acid residues, recombination signal sequences, genomic organization as cladistic markers. From distribution markers conclude that earliest extant tetrapods, amphibians, possess isotypes: κ, σ. Of these, two (κ λ) also found in...
The koala retrovirus (KoRV) is the only known to be in midst of invading germ line its host species. Hybridization capture and next generation sequencing were used on modern museum DNA samples (Phascolarctos cinereus) examine ca. 130 years evolution across full KoRV genome. Overall, entire proviral genome appeared conserved time sequence, protein structure transcriptional binding sites. A total 138 polymorphisms detected, which 72 found more than one individual. At every polymorphic site...
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HspA1A is a cytosolic molecular chaperone essential for cellular homeostasis. also localizes at the plasma membrane (PM) of tumor and stressed cells. However, it currently unknown how this protein translocates to PM. Taking into account that interacts with lipids, including phosphatidylserine (PS), lipids recruit proteins PM, we hypothesized interaction PS allows localize To test hypothesis, subjected cells mild heat-shock PM-localized was quantified using confocal microscopy cell surface...
Abstract HSPA1A, a major heat shock protein, is known to translocate the plasma membrane (PM) in response cellular stress and cancer, where it plays protective roles integrity resistance. Although phosphatidylinositol 4-phosphate [PI(4)P] essential this translocation, signals that trigger facilitate HSPA1A’s movement remain undefined.Given lipid composition dynamically shifts during stress, we hypothesized shock-induced PI(4)P changes are crucial for PM localization. To test hypothesis,...
Abstract The heat shock response (HSR) is a conserved cellular mechanism critical for adaptation to environmental and physiological stressors, with broad implications cell survival, immune responses, cancer biology. While the HSR has been extensively studied at proteomic transcriptomic levels, role of lipid metabolism membrane reorganization remains underexplored. Here, we integrate mass spectrometry-based lipidomics RNA sequencing characterize global lipidomic changes in HeLa cells exposed...
The heat shock response (HSR) is a conserved cellular mechanism critical for adaptation to environmental and physiological stressors, with broad implications cell survival, immune responses, cancer biology. While the HSR has been extensively studied at proteomic transcriptomic levels, role of lipid metabolism membrane reorganization remains underexplored. Here, we integrate mass spectrometry-based lipidomics RNA sequencing characterize global lipidomic changes in HeLa cells exposed three...
Immunoglobulins (or antibodies) are an essential element of the jawed vertebrate adaptive immune response system. These molecules have evolved over past 500 million years and generated highly specialized proteins that recognize extraordinarily large number diverse substances, collectively known as antigens. During evolution diversification immunoglobulin-encoding loci resulted in differences genomic organization, gene content, ratio functional genes pseudogenes. The tinkering process often...
Acinetobacter baumannii A118, a strain isolated from the blood of an infected patient, is naturally competent and unlike most clinical strains, susceptible to variety different antibiotics including those usually used for selection in genetic manipulations. These characteristics make A118 convenient model studies A. baumannii. To identify potential virulence factors, its complete genome was analyzed compared other genomes. includes gene clusters coding acinetobactin baumannoferrin iron...
Abstract Heat shock protein A1A (HSPA1A) is a molecular chaperone crucial in cell survival. In addition to its cytosolic functions, HSPA1A translocates heat-shocked and cancer cells’ plasma membrane (PM). cancer, PM-localized (mHSPA1A) associated with increased tumor aggressiveness therapeutic resistance, suggesting that preventing localization could have value. This translocation depends on HSPA1A’s interaction PM phospholipids, including phosphatidylserine (PS). Although PS binding...