A5030045847- Genomics and Chromatin Dynamics
- Genetic Associations and Epidemiology
- RNA Research and Splicing
- Ubiquitin and proteasome pathways
- Epigenetics and DNA Methylation
- Developmental Biology and Gene Regulation
- Genetic Mapping and Diversity in Plants and Animals
- Genomic variations and chromosomal abnormalities
- Genetic and phenotypic traits in livestock
- Cancer therapeutics and mechanisms
- RNA modifications and cancer
- Cancer-related gene regulation
- BRCA gene mutations in cancer
- Hippo pathway signaling and YAP/TAZ
- Chromosomal and Genetic Variations
- 14-3-3 protein interactions
- Effects of Radiation Exposure
- Kruppel-like factors research
- Wnt/β-catenin signaling in development and cancer
- Pancreatic function and diabetes
- Heme Oxygenase-1 and Carbon Monoxide
- Diabetes Treatment and Management
- PARP inhibition in cancer therapy
- Environmental Chemistry and Analysis
- Health, Environment, Cognitive Aging
Universidade Federal do Sul e Sudeste do Pará
2025
National Institutes of Health
1989-2018
National Human Genome Research Institute
2009-2018
National Institute of Biomedical Imaging and Bioengineering
2018
Emory University
2009-2015
AID Atlanta
2013
Center for Global Health
2012
Seattle University
2009
University of Washington
2009
Fred Hutch Cancer Center
2009
Insulators are protein-bound DNA elements that thought to play a role in chromatin organization and the regulation of gene expression by mediating intra- interchromosomal interactions. Suppressor Hair-wing [Su(Hw)] Drosophila CTCF (dCTCF) insulators found at distinct loci throughout melanogaster genome function recruiting an additional protein, Centrosomal Protein 190 (CP190). We performed immunoprecipitation (ChIP) microarray analysis (ChIP–chip) experiments with whole-genome tiling arrays...
Several multiprotein DNA complexes capable of insulator activity have been identified in Drosophila melanogaster , yet only CTCF, a highly conserved zinc finger protein, and the transcription factor TFIIIC shown to function mammals. CTCF is involved diverse nuclear activities, recent studies suggest that proteins with which it associates sequences targets may underlie these various roles. Here we show homolog (dCTCF) aligns genome other such as Suppressor Hairy wing [SU(HW)] Boundary Element...
Insulin resistance (IR) is a key determinant of type 2 diabetes (T2D) and other metabolic disorders. This genome-wide association study (GWAS) was designed to shed light on the genetic basis fasting insulin (FI) IR in 927 non-diabetic African Americans. 5 396 838 single-nucleotide polymorphisms (SNPs) were tested for associations with FI or adjustments age, sex, body mass index, hypertension status first two principal components. Genotyped SNPs (n = 12) P < × 10(-6) Americans carried forward...
Chronically elevated blood glucose (hyperglycaemia) is the primary indicator of type 2 diabetes, which has a prevalence that varies considerably by ethnicity in USA, with African-Americans disproportionately affected. Genome-wide association studies (GWASs) have significantly enhanced our understanding genetic basis diabetes and related traits, including fasting plasma (FPG). However, majority GWASs been conducted populations European ancestry. Thus, it important to conduct replication...
Total serum bilirubin is associated with several clinical outcomes, including cardiovascular disease, diabetes and drug metabolism. We conducted a genome-wide association study in 619 healthy unrelated African Americans an attempt to replicate reported findings Europeans Asians identify novel loci influencing total levels. analyzed dense panel of over two million genotyped imputed SNPs additive genetic models adjusting for age, sex, the first significant principal components from sample...
Genomic science and associated technologies are providing scientists clinicians with novel insights that transforming the delivery of healthcare overall well-being society. However, these inform us historical population sampling approaches for investigating rare common genetic variations not representative complex ancestral backgrounds today's patients. In order personalized medicine to be meaningful applicable global populations, we will need know how variants found in different parts world...
DNA methylation is a major epigenetic modification important for regulating gene expression and suppressing spurious transcription. Most methods to scan the genome in different tissues differentially methylated sites have focused on of CpGs CpG islands, which are concentrations often associated with promoters.Here, we use profiling strategy that predominantly responsive differences outside islands. The method compares yield from two samples size-selected fragments generated by...
Transcription regulation is mediated by enhancers that bind sequence-specific transcription factors, which in turn interact with the promoters of genes they control. Here, we show JIL-1 kinase present at both and ecdysone-induced Drosophila genes, where it phosphorylates Ser10 Ser28 residues histone H3. also required for CREB binding protein (CBP)-mediated acetylation Lys27, a well-characterized mark active enhancers. The presence these proteins results establishment H3K9acS10ph H3K27acS28ph...
Understanding the relationship between genome organization and expression is central to understanding function. Closely apposed genes in a head-to-head orientation share same upstream region are likely be coregulated. Here we identify Drosophila BEAF-32 insulator as cis regulatory element separating close with different transcription regulation modes. We then compare binding landscapes of protein four genomes highlight evolutionarily conserved presence this adjacent genes. find that changes...
Background Benign ethnic neutropenia (BEN) is a hematologic condition associated with people of African ancestry and specific Middle Eastern groups. Prior genetic association studies in large population showed that rs2814778 Duffy Antigen Receptor for Chemokines (DARC) gene, specifically DARC null red cell phenotype, was BEN. However, the mechanism this phenotype leading to low white count remained elusive. Methods We conducted an extreme design genome-wide study (GWAS), analyzed ~16 million...
Chromatin insulators have been implicated in the regulation of higher-order chromatin structure and may function to compartmentalize eukaryotic genome into independent domains gene expression. To test this possibility, we used biochemical computational approaches identify gypsy-like genomic-binding sites for Suppressor Hairy-wing [Su(Hw)] protein, a component gypsy insulator. EMSA FISH analyses suggest that these are genuine Su(Hw)-binding sites. In addition, functional tests indicate...
Rhamnolipids, biosurfactants synthesized from natural resources, demonstrate significant applications, including notable insecticidal efficacy against Aedes aegypti L., the primary vector for numerous arboviruses. The global spread of A. poses substantial public health challenges, requiring innovative and sustainable control strategies. This research investigates use andiroba (Carapa guianensis Aubl.) biomass waste as a substrate synthesizing rhamnolipid biosurfactant (BSAW) produced by...
Biossurfactantes (BS) são moléculas de relevância industrial significativa, reconhecidas por suas propriedades tensoativas e potencial para produção microrganismos que utilizam fontes alternativas carbono energia. O cacau (Theobroma cacao L.), fruto amazônico amplamente utilizado como matéria-prima na chocolate, representa um recurso alternativo exemplar. Apesar da falta valor econômico atribuído aos resíduos provenientes do processamento cacau, esses subprodutos podem ser substratos...
Brd4 is a double bromodomain protein that has been shown to interact with acetylated histones regulate transcription by recruiting Positive Transcription Elongation Factor b the promoter region. also involved in gene bookmarking during mitosis and therapeutic target for treatment of acute myeloid leukemia. The Drosophila melanogaster homologue called Fs(1)h and, like its vertebrate counterpart, encodes different isoforms. We have used ChIP-seq examine genome-wide distribution are able...
In order to eliminate health disparities in the United States, more efforts are needed address breadth of social issues directly contributing healthy divide observed across racial and ethnic groups. Socioeconomic status, education, environment intimately linked outcomes. However, with tremendous advances technology increased investigation into human genetic variation, genomics is poised play a valuable role bolstering find new treatments preventions for chronic conditions diseases that...
Insulators are DNA sequences thought to be important for the establishment and maintenance of cell-type specific nuclear architecture. In Drosophila there several classes insulators that appear have unique roles in gene expression. The mechanisms involved determining regulating these insulator not understood. Here we report Topoisomerase II modulates activity Su(Hw) insulator. Downregulation Topo by RNAi or mutations Top2 result disruption function. This effect is mediated Mod(mdg4)2.2...
In Drosophila, dosage compensation is mediated by the MSL complex, which binds numerous sites on X chromosome in males and enhances transcriptional rate of a substantial number X-linked genes. We have determined that topoisomerase II (Topo II) enriched compensated genes, to it recruited association with excess amount present autosomal genes similar transcription levels. Using plasmid model, we show Topo required for proper chromatin topologically different from non-compensated chromatin....