A5000037407- Antibiotic Resistance in Bacteria
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Enzyme Production and Characterization
- Microbial Community Ecology and Physiology
- Peptidase Inhibition and Analysis
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- Glycosylation and Glycoproteins Research
- Microbial Natural Products and Biosynthesis
- Antibiotics Pharmacokinetics and Efficacy
- Bacteriophages and microbial interactions
- Plant Disease Resistance and Genetics
- Protein Structure and Dynamics
- Carbohydrate Chemistry and Synthesis
- Antibiotic Use and Resistance
- Plant-Microbe Interactions and Immunity
- Pneumonia and Respiratory Infections
- Probiotics and Fermented Foods
- ATP Synthase and ATPases Research
- Biochemical and Structural Characterization
- Amino Acid Enzymes and Metabolism
- Escherichia coli research studies
- Pharmaceutical and Antibiotic Environmental Impacts
University of Liège
2016-2025
Wesleyan University
2009
Boston Biomedical Research Institute
2004-2007
Fundación Ramón Domínguez
2004-2006
Wiskott-Aldrich syndrome protein (WASP)-homology domain 2 (WH2) is a small and widespread actin-binding motif. In the WASP family, WH2 plays role in filament nucleation by Arp2/3 complex. Here we describe crystal structures of complexes actin with domains WASP, WASP-family verprolin homologous protein, WASP-interacting protein. Despite low sequence identity, shares structural similarity N-terminal portion monomer-sequestering thymosin beta (Tbeta). We show that both inhibit nucleotide...
We solved the crystal structure of a secreted protein, EXLX1, encoded by yoaJ gene Bacillus subtilis . Its is remarkably similar to that plant β-expansins (group 1 grass pollen allergens), consisting 2 tightly packed domains (D1, D2) with potential polysaccharide-binding surface spanning domains. Domain D1 has double-ψ β-barrel fold partial conservation catalytic site found in family 45 glycosyl hydrolases and MltA lytic transglycosylases. D2 an Ig-like group 2/3 allergens, structural...
Binary fission is the ultimate step of prokaryotic cell cycle. In Gram-negative bacteria like Escherichia coli, this implies invagination three biological layers (cytoplasmic membrane, peptidoglycan and outer membrane), biosynthesis new poles eventually, daughter cells separation. The latter requires coordinated action N-acetylmuramyl-L-alanine amidases AmiA/B/C their LytM activators EnvC NlpD to cleave septal peptidoglycan. We present here 2.5 Å crystal structure AmiC which includes first...
In Escherichia coli, penicillin-binding protein 3 (PBP3), also known as FtsI, is a central component of the divisome, catalyzing cross-linking cell wall peptidoglycan during division. PBP3 mainly periplasmic, with 23 residues cytoplasmic tail and single transmembrane helix. We have solved crystal structure soluble form (PBP357–577) at 2.5 Å revealing two modules high molecular weight class B PBPs, carboxy terminal module exhibiting transpeptidase activity an amino unknown function. To gain...
Abstract Streptomyces scabies is an economically important plant pathogen well-known for damaging root and tuber crops by causing scab lesions. Thaxtomin A the main causative agent responsible pathogenicity of S. cello-oligosaccharides are environmental triggers that induce production this phytotoxin. How sensed or transported in order to virulent behavior ? Here we report cellobiose cellotriose binding protein CebE MsiK, ATPase providing energy carbohydrates transport, protagonists...
Contaminating sequences in public genome databases is a pervasive issue with potentially far-reaching consequences. This problem has attracted much attention the recent literature and many different tools are now available to detect contaminants. Although these methods based on diverse algorithms that can sometimes produce widely estimates of contamination level, majority genomic studies rely single method detection, which represents risk systematic error. In this work, we used two...
ABSTRACT The peptidoglycan (PG, or murein) is a mesh-like structure, which made of glycan polymers connected by short peptides and surrounds the cell membrane nearly all bacterial species. In contrast, there no PG counterpart that would be universally found in Archaea but rather various are specific to some lineages. Methanopyrales Methanobacteriales two orders Euryarchaeota harbor pseudomurein (PM), structural analog PG. Owing differences between PM biosynthesis, have argued origin both not...
Abstract The Arabidopsis splicing factor serine/arginine-rich 45 (SR45) contributes to several biological processes. sr45-1 loss-of-function mutant exhibits delayed root development, late flowering, unusual numbers of floral organs, shorter siliques with decreased seed sets, narrower leaves and petals, altered metal distribution. SR45 bears a unique RNA recognition motif (RRM) flanked by one (RS) domain on both sides. Here, we studied the function each domains examining their involvement in:...
In this study, the crystal structure of a class C β‐lactamase from psychrophilic organism, Pseudomonas fluorescens , has been refined to 2.2 Å resolution. It is one few solved structures proteins. The was compared with those homologous mesophilic enzymes and another, modeled, protein. elucidation 3D enzyme provides additional insights into features involved in cold adaptation. Structure comparison β‐lactamases shows that electrostatics seems play major role low‐temperature adaptation, lower...
The catalytic efficiency of the class D β-lactamase OXA-10 depends critically on an unusual carboxylated lysine as general base residue for both enzyme acylation and deacylation steps catalysis. Evidence is presented that interaction between indole group Trp154 essential stability posttranslationally modified Lys70. Substitution by Gly, Ala, or Phe yielded noncarboxylated enzymes which displayed poor efficiencies reduced when compared to wild-type OXA-10. W154H mutant was partially...
The activity of class D β-lactamases is dependent on Lys70 carboxylation in the active site. Structural, kinetic and affinity studies show that this post-translational modification can be affected by presence a poor substrate such as moxalactam but also V117T substitution. Val117 strictly conserved hydrophobic residue located In addition, inhibition chloride ions due to competition between side chain carboxylate modified ions. Determination individual constants shows deacylation acyl-enzyme...
Boronic acids bearing appropriate side chains are good inhibitors of serine amidohydrolases. The boron usually adopts a tetrahedral conformation, bound to the nucleophilic active site and mimicking transition state enzymatic reaction. We have solved structures complexes penicillin-binding protein, DD-peptidase from Actinomadura sp. R39, with four amidomethylboronic (2,6-dimethoxybenzamidomethylboronic acid, phenylacetamidomethylboronic 2-chlorobenzamidomethylboronic...
Several integral membrane proteins exhibiting undecaprenyl-pyrophosphate (C55-PP) phosphatase activity were previously identified in Escherichia coli that belonged to two distinct protein families: the BacA protein, which accounts for 75% of C55-PP detected E. cell membranes, and three members PAP2 phosphatidic acid family, namely PgpB, YbjG LpxT. This dephosphorylation step is required provide C55-P carrier lipid plays a central role biosynthesis various wall polymers. We here report...
Plant colonization by Streptomyces scabiei, the main cause of common scab disease on root and tuber crops, is triggered cello-oligosaccharides, cellotriose being most efficient elicitor. The import cello-oligosaccharides via ATP-binding cassette (ABC) transporter CebEFG-MsiK induces production thaxtomin phytotoxins, central virulence determinants this species, as well many other metabolites that compose 'virulome' S. scabiei. Homology searches revealed paralogues CebEFG proteins, encoded...
As in several staphylococci, the synthesis of Bacillus licheniformis 749/I β-lactamase is an inducible phenomenon regulated by a signal-transducing membrane protein BlaR. The C-terminal domain this multimodular extracellular which specifically recognizes β-lactam antibiotics. When it binds β-lactam, signal transmitted transmembrane region to intracellular loops. In response, hydrolytic activity BlaR large cytoplasmic L3 loop induced, and cascade reactions generated, leading transcription...